Featured Research

from universities, journals, and other organizations

Method keeps surgically-removed prostate tissue alive and 'working' for week

Date:
November 4, 2010
Source:
Johns Hopkins Medical Institutions
Summary:
Scientists have developed a technique to keep normal and cancerous prostate tissue removed during surgery alive and functioning normally in the laboratory for up to a week.

Scientists at the Johns Hopkins Kimmel Cancer Center, University of Helsinki and Stanford University have developed a technique to keep normal and cancerous prostate tissue removed during surgery alive and functioning normally in the laboratory for up to a week.

Related Articles


The new technique could not only enhance research of prostate biology and cancer, but also hasten the creation of individualized medicines for prostate cancer patients, the investigators say. Previous attempts to culture live prostate tissues resulted in poor viability and lost "tissue architecture," the researchers note, making them less than useful for research or therapy development.

"Our technique could help scientists more accurately predict how living prostate tissues respond to therapy," says Marikki Laiho, M.D., Ph.D., director of the division of Molecular Radiation Sciences at Johns Hopkins. "It holds promise for testing anticancer drugs that work best."

For the study, published in the Nov. 1 issue of Cancer Research, the scientists refined their multistep tissue culture technique and performed experiments to test the tissues' viability and utility in research. Laiho worked with Stanford University researcher Donna Peehl, Ph.D., to pilot the technique in a research project completed in 2007.

Customarily, pathologists store tissue samples in paraffin wax, which kills the tissue, resulting in samples that are essentially frozen in time. In many research laboratories, scientists experiment with prostate cancer cells that have been grown in flasks filled with nutrients and kept under strict temperature conditions. But these cells are not connected together in the tightly knit architecture of tissue that exists in the actual prostate gland.

"Tissue architecture may hold clues to why certain therapies work and others fail, and may be a better model of the intact, in vivo prostate gland," says Laiho, who is the Willard and Lillian Hackerman Professor of Radiation Oncology at Johns Hopkins.

Laiho says that one key to success for the international team was to work with surgeons and pathologists to speed up delivery of tissue samples to the pathology lab from the operating room.

At the pathology lab, scientists cut thin slices of prostate specimens taken from 18 patients who had undergone surgery on the prostate gland at the Helsinki University Central Hospital or The Johns Hopkins Hospital during 2007-2009.

Specimen slices had to be a precise thickness to allow cells throughout the tissue to maintain a healthy exchange of gases and growth factors.

Then, Laiho and her team placed the tissues in a liquid solution comprised of a complex mix of 64 separate ingredients to maintain the proper chemical and nutritional support for the biological functions in the tissue.

The scientists validated the presence of biomarkers specific for each type of cell within the prostate tissues to ensure that they were viable. The scientists caution that although their method gives them a more "real-life" model of the prostate with live tissue samples, it comes at a cost: -- even with support, the tissues are short-lived, and experiments on fresh specimens must be completed within one week, which may be too short for some types of research.

The Hopkins-Helsinki team has already used their tissue-culture technique to measure levels of proteins known to repair DNA damage caused by carcinogens and other environmental agents. They found that one of these proteins -- p53 -- is not activated consistently enough to repair DNA damage. They also found that one of the first proteins to arrive on the DNA repair scene -- H2AX -- is activated at expected levels in all but one of the architectural compartments in prostate tissue. Low levels of H2AX were found in the so-called "luminal" compartment of prostate tissue, in the part of the prostate gland that produces secretions to protect sperm cells.

Laiho says the tissue-culture technique was a key component of understanding which DNA repair proteins may or may not be activated in different parts of prostate tissue and could help scientists develop therapies that target these DNA repair proteins.

The Hopkins and Helsinki investigators plan to use their new tissue-culture technique to test the response of experimental drugs on prostate cancer tissues.

Funding for the study was provided by the Academy of Finland, Patrick C. Walsh Prostate Cancer Research Fund, K. Albin Johansson Foundation, Biomedicum Helsinki Foundation, Finnish-Norwegian Medical Foundation, Finnish Medical Foundation and Helsinki Biomedical Graduate School.

Scientists contributing to the research included Sari Jäämaa, Taija M. af Hällström, Anna Sankila, Ville Rantanen, Hannu Koistinen, Ulf-Håkan Stenman from the University of Helsinki, Finland; Zhewei Zhang, Zhiming Yang, and Angelo De Marzo from Johns Hopkins; Kimmo Taari and Mirja Ruutu from the Helsinki University Central Hospital; and Leif C. Andersson from the University of Helsinki and the Karolinska Institutet in Sweden.


Story Source:

The above story is based on materials provided by Johns Hopkins Medical Institutions. Note: Materials may be edited for content and length.


Journal Reference:

  1. S. Jaamaa, T. M. af Hallstrom, A. Sankila, V. Rantanen, H. Koistinen, U.-H. Stenman, Z. Zhang, Z. Yang, A. M. De Marzo, K. Taari, M. Ruutu, L. C. Andersson, M. Laiho. DNA Damage Recognition via Activated ATM and p53 Pathway in Nonproliferating Human Prostate Tissue. Cancer Research, 2010; 70 (21): 8630 DOI: 10.1158/0008-5472.CAN-10-0937

Cite This Page:

Johns Hopkins Medical Institutions. "Method keeps surgically-removed prostate tissue alive and 'working' for week." ScienceDaily. ScienceDaily, 4 November 2010. <www.sciencedaily.com/releases/2010/11/101103135438.htm>.
Johns Hopkins Medical Institutions. (2010, November 4). Method keeps surgically-removed prostate tissue alive and 'working' for week. ScienceDaily. Retrieved October 24, 2014 from www.sciencedaily.com/releases/2010/11/101103135438.htm
Johns Hopkins Medical Institutions. "Method keeps surgically-removed prostate tissue alive and 'working' for week." ScienceDaily. www.sciencedaily.com/releases/2010/11/101103135438.htm (accessed October 24, 2014).

Share This



More Health & Medicine News

Friday, October 24, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Breakfast Debate: To Eat Or Not To Eat?

Breakfast Debate: To Eat Or Not To Eat?

Newsy (Oct. 23, 2014) — Conflicting studies published in the same week re-ignited the debate over whether we should be eating breakfast. Video provided by Newsy
Powered by NewsLook.com
Ebola Fears Keep Guinea Hospitals Empty

Ebola Fears Keep Guinea Hospitals Empty

AP (Oct. 23, 2014) — Fears of Ebola are keeping doctors and patients alike away from hospitals in the West African nation of Guinea. (Oct. 23) Video provided by AP
Powered by NewsLook.com
Despite Rising Death Toll, Many Survive Ebola

Despite Rising Death Toll, Many Survive Ebola

AP (Oct. 23, 2014) — The family of a Dallas nurse infected with Ebola in the US says doctors can no longer detect the virus in her. Despite the mounting death toll in West Africa, there are survivors there too. (Oct. 23) Video provided by AP
Powered by NewsLook.com
Orthodontist Mom Jennifer Salzer on the Best Time for Braces

Orthodontist Mom Jennifer Salzer on the Best Time for Braces

Working Mother (Oct. 22, 2014) — Is your child ready? Video provided by Working Mother
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
 
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:  

Breaking News:

Strange & Offbeat Stories

 

Health & Medicine

Mind & Brain

Living & Well

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:  

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile iPhone Android Web
Follow Facebook Twitter Google+
Subscribe RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins