Featured Research

from universities, journals, and other organizations

Human methylome sequenced at single base-pair resolution

Date:
November 10, 2010
Source:
Public Library of Science
Summary:
DNA methylation plays an important role in many processes such as animal development, X-chromosome inactivation, and carcinogenesis. Understanding the mechanisms and functions of DNA methylation and how it varies from tissue to tissue and between individuals will have profound implications for human health and disease. A team of Chinese researchers decoded the essentially complete methylome (an inventory of all the bases that are methylated) of the human genome using peripheral blood mononuclear cells (PBMCs).

DNA methylation plays an important role in many processes such as animal development, X-chromosome inactivation, and carcinogenesis. Understanding the mechanisms and functions of DNA methylation and how it varies from tissue to tissue and between individuals will have profound implications for human health and disease.

A team of Chinese researchers decoded the essentially complete methylome (an inventory of all the bases that are methylated) of the human genome using peripheral blood mononuclear cells (PBMCs). The results are published in the online, open access journal PLoS Biology.

The research is part of YanHuang (YH) Project, which has been launched by BGI (previous known as Beijing Genomics Institute) at Shenzhen, which aims to sequence 100 Chinese individuals in 3 years to accelerate the discovery of disease genes and mutations in an Asian population.

The methylome was generated from the same donor whose genome was deciphered in the YH project. The methylome was examined at 20 distinct features including regulatory, protein-coding, non-coding, and repeat sequences. The integration of the data with the previously determined genome sequence of the same Asian individual allowed the identification of allele-specific methylation (ASM) differences between the methylomes of the genomes inherited from either parent. This revealed that ASM was highly correlated with allele-specific gene expression (ASE) which indicated that parental gene imprinting (that is the favored expression of the genes inherited from one parent) may be more common than previously thought.

The research not only provides a comprehensive resource for future epigenomic research but also demonstrates a paradigm for epigenetic studies through new sequencing technology. The PBMC methylome data has been deposited to NCBI (http://www.ncbi.nlm.nih.gov/Traces/sra/, accession number: SRA008544). It is expected to form a lasting resource as part of the International Human Epigenome Project.


Story Source:

The above story is based on materials provided by Public Library of Science. Note: Materials may be edited for content and length.


Journal Reference:

  1. Wolf Reik, Yingrui Li, Jingde Zhu, Geng Tian, Ning Li, Qibin Li, Mingzhi Ye, Hancheng Zheng, Jian Yu, Honglong Wu, Jihua Sun, Hongyu Zhang, Quan Chen, Ruibang Luo, Minfeng Chen, Yinghua He, Xin Jin, Qinghui Zhang, Chang Yu, Guangyu Zhou, Jinfeng Sun, Yebo Huang, Huisong Zheng, Hongzhi Cao, Xiaoyu Zhou, Shicheng Guo, Xueda Hu, Xin Li, Karsten Kristiansen, Lars Bolund, Jiujin Xu, Wen Wang, Huanming Yang, Jian Wang, Ruiqiang Li, Stephan Beck, Jun Wang, Xiuqing Zhang. The DNA Methylome of Human Peripheral Blood Mononuclear Cells. PLoS Biology, 2010; 8 (11): e1000533 DOI: 10.1371/journal.pbio.1000533

Cite This Page:

Public Library of Science. "Human methylome sequenced at single base-pair resolution." ScienceDaily. ScienceDaily, 10 November 2010. <www.sciencedaily.com/releases/2010/11/101109172339.htm>.
Public Library of Science. (2010, November 10). Human methylome sequenced at single base-pair resolution. ScienceDaily. Retrieved July 29, 2014 from www.sciencedaily.com/releases/2010/11/101109172339.htm
Public Library of Science. "Human methylome sequenced at single base-pair resolution." ScienceDaily. www.sciencedaily.com/releases/2010/11/101109172339.htm (accessed July 29, 2014).

Share This




More Health & Medicine News

Tuesday, July 29, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Deadly Ebola Virus Threatens West Africa

Deadly Ebola Virus Threatens West Africa

AP (July 28, 2014) West African nations and international health organizations are working to contain the largest Ebola outbreak in history. It's one of the deadliest diseases known to man, but the CDC says it's unlikely to spread in the U.S. (July 28) Video provided by AP
Powered by NewsLook.com
$15B Deal on Vets' Health Care Reached

$15B Deal on Vets' Health Care Reached

AP (July 28, 2014) A bipartisan deal to improve veterans health care would authorize at least $15 billion in emergency spending to fix a veterans program scandalized by long patient wait times and falsified records. (July 28) Video provided by AP
Powered by NewsLook.com
Two Americans Contract Ebola in Liberia

Two Americans Contract Ebola in Liberia

Reuters - US Online Video (July 28, 2014) Two American aid workers in Liberia test positive for Ebola while working to combat the deadliest outbreak of the virus ever. Linda So reports. Video provided by Reuters
Powered by NewsLook.com
Traditional African Dishes Teach Healthy Eating

Traditional African Dishes Teach Healthy Eating

AP (July 28, 2014) Classes are being offered nationwide to encourage African Americans to learn about cooking fresh foods based on traditional African cuisine. The program is trying to combat obesity, heart disease and other ailments often linked to diet. (July 28) Video provided by AP
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:

Breaking News:
from the past week

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile: iPhone Android Web
Follow: Facebook Twitter Google+
Subscribe: RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins