Unlike heart or brain injuries, which show obvious outward signs, physical symptoms are not typically present with AKI. Researchers have been looking for markers of AKI, with the hope that early detection will lead to early therapy to prevent kidney failure. Richard Zager, MD (Clinical Research Division, Fred Hutchinson Cancer Research Center) and his colleagues investigated whether certain molecules that are produced during injury and infection might be excreted in the urine and serve as diagnostic markers. Specifically, they measured the diagnostic potential of monocyte chemoattractant protein-1, a protein that plays a role in recruiting immune cells to injured or infected sites in the body. This protein has been found in the joints of people with rheumatoid arthritis and in the urine of people with lupus.

The investigators found elevated levels of monocyte chemoattractant protein-1 as well as its mRNA (the template for protein synthesis) in urine samples from both mice and human patients with AKI. This suggests that the gene that encodes this mRNA and protein is activated in patients with AKI. Using a new technique, known as chromatin immunoprecipitation assay, the investigators were also able to show changes in proteins (known as histones) that can activate the gene that produces MCP-1. This is the first time that the ability to detect these protein modifiers have been identified in human urine samples.

"This is a new diagnostic test that provides information about what processes are actually inducing acute kidney injury; however, a much larger prospective study is required to ultimately determine clinical utility," said Dr. Zager.

Study co-authors include Raj Munshi, MD (Seattle Children's Hospital and Regional Medical Center); Ali Johnson, PhD (Fred Hutchinson Cancer Research Center); Edward Siew, MD, T. Alp Ikizler, MD, Lorraine Ware, MD (Vanderbilt University Medical Center); and Mark Wurfel, MD, and Jonathan Himmelfarb, MD (University of Washington).

Note: If no author is given, the source is cited instead.

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