Featured Research

from universities, journals, and other organizations

The ultimate qPCR based lncRNA profiling platform

Date:
October 19, 2011
Source:
Ghent University
Summary:
lncRNAs represent an emerging important class of regulatory transcribed elements. The study of lncRNAs is timely and relevant, because of their implication in important cellular processes, through new modes of action. The lncRNA genes are generally low abundant, which is exactly why there is a genuine need for a platform with ultimate sensitivity superseding microarray based platforms.

lncRNAs represent an emerging important class of regulatory transcribed elements. The study of lncRNAs is timely and relevant, because of their implication in important cellular processes, through new modes of action. The lncRNA genes are generally low abundant, which is exactly why there is a genuine need for a platform with ultimate sensitivity superseding microarray based platforms.

Related Articles


Biogazelle has developed a revolutionary platform for accurate and sensitive expression profiling of 1718 human long non-coding RNAs (lncRNAs). The platform consists of state-of-the-art validated qPCR assays for 1666 long intergenic ncRNAs (lincRNAs) and 52 lncRNAs.

Experiments are set-up in 5184-well SmartChips (Wafergen). A low-volume liquid handler enables fast and high-precision pipetting and low volume qPCR reactions to ensure fast turn-around times at affordable costs. The 1718 SYBR Green I RT-qPCR assays are strictly validated according to the MIQE guidelines, and meet Biogazelle's strict quality control criteria in terms of optimal PCR efficiency, specificity, sensitivity and linear dynamic range of quantification.

Data analysis is performed using our qbasePLUS software, which is built upon a state-of-the-art quantification model. Thorough post-PCR quality control ensures reliable results. The calculation parameters and quality control settings are tailored toward the need of the lincRNA project. Both raw data and processed results (e.g. global mean normalized relative quantities) are delivered in an easy to understand format.


Story Source:

The above story is based on materials provided by Ghent University. Note: Materials may be edited for content and length.


Cite This Page:

Ghent University. "The ultimate qPCR based lncRNA profiling platform." ScienceDaily. ScienceDaily, 19 October 2011. <www.sciencedaily.com/releases/2011/10/111019122900.htm>.
Ghent University. (2011, October 19). The ultimate qPCR based lncRNA profiling platform. ScienceDaily. Retrieved November 27, 2014 from www.sciencedaily.com/releases/2011/10/111019122900.htm
Ghent University. "The ultimate qPCR based lncRNA profiling platform." ScienceDaily. www.sciencedaily.com/releases/2011/10/111019122900.htm (accessed November 27, 2014).

Share This


More From ScienceDaily



More Plants & Animals News

Thursday, November 27, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

New Dinosaur Species Found in Museum Collection

New Dinosaur Species Found in Museum Collection

Reuters - Innovations Video Online (Nov. 27, 2014) A British palaeontologist has discovered a new species of dinosaur while studying fossils in a Canadian museum. Pentaceratops aquilonius was related to Triceratops and lived at the end of the Cretaceous Period, around 75 million years ago. Jim Drury has more. Video provided by Reuters
Powered by NewsLook.com
Classic Hollywood Memorabilia Goes Under the Hammer

Classic Hollywood Memorabilia Goes Under the Hammer

Reuters - Entertainment Video Online (Nov. 26, 2014) The iconic piano from "Casablanca" and the Cowardly Lion suit from "The Wizard of Oz" fetch millions at auction. Sara Hemrajani reports. Video provided by Reuters
Powered by NewsLook.com
Pet Dogs to Be Used in Anti-Ageing Trial

Pet Dogs to Be Used in Anti-Ageing Trial

Reuters - Innovations Video Online (Nov. 26, 2014) Researchers in the United States are preparing to discover whether a drug commonly used in human organ transplants can extend the lifespan and health quality of pet dogs. Video provided by Reuters
Powered by NewsLook.com
From Popcorn To Vending Snacks: FDA Ups Calorie Count Rules

From Popcorn To Vending Snacks: FDA Ups Calorie Count Rules

Newsy (Nov. 25, 2014) The US FDA is announcing new calorie rules on Tuesday that will require everywhere from theaters to vending machines to include calorie counts. Video provided by Newsy
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:

Breaking News:

Strange & Offbeat Stories


Plants & Animals

Earth & Climate

Fossils & Ruins

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile: iPhone Android Web
Follow: Facebook Twitter Google+
Subscribe: RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins