Featured Research

from universities, journals, and other organizations

Growing where no cell has grown before: Unique suspension technique developed for large-scale stem cell production

Date:
April 5, 2012
Source:
University of Toronto
Summary:
Researchers have developed a unique new technique for growing stem cells that may make possible cost-effective, large-scale stem cell manufacturing and research.

Not all stem cells are created equally, or so David Fluri, IBBME postdoctoral researcher, has discovered.

Related Articles


Working under IBBME Professor Peter Zandstra at the Terrence Donnelly Centre for Cellular and Biomolecular Research at the University of Toronto, Fluri has developed a unique technique for growing large volumes of stem cells. Although stem cells are widely used for the testing of new drugs, researchers have always faced difficulties manufacturing enough viable cells from a culture. Typically, stem cells are grown on surfaces that must be scraped, and which must then be differentiated from other types of cells to prevent the death of those all-important cells. This has proven to be an inefficient way to harvest stem cells, since the process doesn't produce the cells in high enough numbers that they can be cost-effectively used by researchers.

Wanting to overcome this challenge, Fluri decided to pair the stem cell creation process, known as reprogramming, with the use of a bioreactor -- an apparatus that creates stable environmental conditions. Through this process Fluri was able to "reprogram" mouse cells to become "pluripotent" stem cells -- cells that can become any kind of cell -- and then into cardiac cells.

But it was the way these cells were successfully grown that has researchers excited.

By introducing the cultures to this particular bioreactor process, the stem cells were grown in suspension, eliminating the problems inherent in growing the cells on surfaces -- something that has never before been accomplished. "This is an enabling technology," says Prof. Zandstra of the discovery. "[It] takes something we showed we could do before at low efficiency but not at such numbers that could be used in manufacturing."

Fluri hopes his discovery, which is "more compatible with large scale processes" will help ease the "bottleneck of cell production" used for research and drug development. The results of his have just been released in the high profile scientific research journal, Nature Methods.

Yet there are greater implications involved in Fluri's discovery: once stem cells are produced, they are "differentiated" to become other kinds of cells -- cardiac cells, for instance -- and Fluri's new growing process has the potential to make this phase of cell production safer and more stable.

Fluri specifically experimented with the integrated differentiation of stem cells into cardiac cells, which could then be used for drug screening and identifying new drugs for cardiac diseases. But created in large enough numbers -- and translated to human models -- the process may one day be used to treat heart disease.


Story Source:

The above story is based on materials provided by University of Toronto. Note: Materials may be edited for content and length.


Journal Reference:

  1. David A Fluri, Peter D Tonge, Hannah Song, Ricardo P Baptista, Nika Shakiba, Shreya Shukla, Geoffrey Clarke, Andras Nagy, Peter W Zandstra. Derivation, expansion and differentiation of induced pluripotent stem cells in continuous suspension cultures. Nature Methods, 2012; DOI: 10.1038/nmeth.1939

Cite This Page:

University of Toronto. "Growing where no cell has grown before: Unique suspension technique developed for large-scale stem cell production." ScienceDaily. ScienceDaily, 5 April 2012. <www.sciencedaily.com/releases/2012/04/120405224849.htm>.
University of Toronto. (2012, April 5). Growing where no cell has grown before: Unique suspension technique developed for large-scale stem cell production. ScienceDaily. Retrieved December 18, 2014 from www.sciencedaily.com/releases/2012/04/120405224849.htm
University of Toronto. "Growing where no cell has grown before: Unique suspension technique developed for large-scale stem cell production." ScienceDaily. www.sciencedaily.com/releases/2012/04/120405224849.htm (accessed December 18, 2014).

Share This


More From ScienceDaily



More Health & Medicine News

Thursday, December 18, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Kids Die While Under Protective Services

Kids Die While Under Protective Services

AP (Dec. 18, 2014) As part of a six-month investigation of child maltreatment deaths, the AP found that hundreds of deaths from horrific abuse and neglect could have been prevented. AP's Haven Daley reports. (Dec. 18) Video provided by AP
Powered by NewsLook.com
UN: Up to One Million Facing Hunger in Ebola-Hit Countries

UN: Up to One Million Facing Hunger in Ebola-Hit Countries

AFP (Dec. 17, 2014) Border closures, quarantines and crop losses in West African nations battling the Ebola virus could lead to as many as one million people going hungry, UN food agencies said on Wednesday. Duration: 00:52 Video provided by AFP
Powered by NewsLook.com
When You Lose Weight, This Is Where The Fat Goes

When You Lose Weight, This Is Where The Fat Goes

Newsy (Dec. 17, 2014) Can fat disappear into thin air? New research finds that during weight loss, over 80 percent of a person's fat molecules escape through the lungs. Video provided by Newsy
Powered by NewsLook.com
Why Your Boss Should Let You Sleep In

Why Your Boss Should Let You Sleep In

Newsy (Dec. 17, 2014) According to research out of the University of Pennsylvania, waking up for work is the biggest factor that causes Americans to lose sleep. Video provided by Newsy
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:

Breaking News:

Strange & Offbeat Stories


Health & Medicine

Mind & Brain

Living & Well

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile: iPhone Android Web
Follow: Facebook Twitter Google+
Subscribe: RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins