Featured Research

from universities, journals, and other organizations

DNA vaccine and duck eggs protect against hantavirus disease

Date:
May 23, 2012
Source:
US Army Medical Research Institute of Infectious Diseases
Summary:
Scientists have successfully protected laboratory animals from lethal hantavirus disease using a novel approach that combines DNA vaccines and duck eggs.

Army scientists and industry collaborators have successfully protected laboratory animals from lethal hantavirus disease using a novel approach that combines DNA vaccines and duck eggs. The work appears in a recent edition of the online scientific journal PLoS ONE, published by the Public Library of Science.

According to first author Jay W. Hooper of the U.S. Army Medical Research Institute of Infectious Diseases (USAMRIID), this is the first time that the DNA vaccine/duck egg system has been shown to produce an antiviral product capable of protecting against hantavirus disease.

Hantavirus causes a condition known as hantavirus pulmonary syndrome (HPS), which has a case fatality rate of 35-40 percent. Currently there are no vaccines, prophylactics, or therapeutics to prevent or treat this highly pathogenic disease.

In this study, the research team used a hamster model of Andes virus, which is the predominant cause of HPS in South America and the only hantavirus known to be transmitted person-to-person. Infection of Syrian hamsters with Andes virus, as demonstrated in earlier studies at USAMRIID, results in a disease that closely mimics human HPS in incubation time, symptoms of respiratory distress, and disease pathology. This makes it an ideal system for evaluating the feasibility of postexposure protection strategies.

Collaborating with Aldevron of Fargo, N.D. and the Universidad del Desarrollo in Santiago, Chile, Hooper and his team first evaluated a natural product, human polyclonal antibody, which was obtained as fresh frozen plasma (FFP) from a patient who survived HPS. Their results indicate that FFP shows promise as a post-exposure preventive treatment for HPS.

The team then vaccinated ducks with a DNA vaccine against Andes virus. This vaccine, initially developed and tested at USAMRIID, uses genetic material, or DNA, that encodes a specific hantavirus gene to elicit an immune response in the recipient.

Next, they purified an antibody called IgY from the yolks of the duck eggs. This purified IgY, as well as a similar version produced in duck eggs, was capable of neutralizing Andes virus when tested in cell culture. More importantly, it also protected Syrian hamsters from lethal HPS -- even when administered as a single injection several days after the hamsters had been exposed to a lethal dose of virus.

The work demonstrates the feasibility of using DNA vaccine technology, coupled with the duck/egg system, to manufacture a product that could supplement or replace FFP. Furthermore, the new approach can be scaled as needed and eliminates the necessity of using blood products from HPS survivors, which may be in limited supply.

According to Hooper, another advantage of this technique is that duck IgY naturally loses a part of the antibody that has been associated with "serum sickness" when animal antibodies have been used in humans, making the product potentially less reactogenic.

"This antiviral product, if fully developed and manufactured, has the potential to be used in future outbreak situations," Hooper said. "It also could be used to treat health care workers and others who have close contact with HPS patients."

In addition, the authors suggest, the flexibility of the DNA vaccine/duck egg system could be applied to the production of antibodies against other infectious agents and toxins.


Story Source:

The above story is based on materials provided by US Army Medical Research Institute of Infectious Diseases. Note: Materials may be edited for content and length.


Journal Reference:

  1. Rebecca Brocato, Matthew Josleyn, John Ballantyne, Pablo Vial, Jay W. Hooper. DNA Vaccine-Generated Duck Polyclonal Antibodies as a Postexposure Prophylactic to Prevent Hantavirus Pulmonary Syndrome (HPS). PLoS ONE, 2012; 7 (4): e35996 DOI: 10.1371/journal.pone.0035996

Cite This Page:

US Army Medical Research Institute of Infectious Diseases. "DNA vaccine and duck eggs protect against hantavirus disease." ScienceDaily. ScienceDaily, 23 May 2012. <www.sciencedaily.com/releases/2012/05/120523115047.htm>.
US Army Medical Research Institute of Infectious Diseases. (2012, May 23). DNA vaccine and duck eggs protect against hantavirus disease. ScienceDaily. Retrieved October 21, 2014 from www.sciencedaily.com/releases/2012/05/120523115047.htm
US Army Medical Research Institute of Infectious Diseases. "DNA vaccine and duck eggs protect against hantavirus disease." ScienceDaily. www.sciencedaily.com/releases/2012/05/120523115047.htm (accessed October 21, 2014).

Share This



More Plants & Animals News

Tuesday, October 21, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

White Lion Cubs Born in Belgrade Zoo

White Lion Cubs Born in Belgrade Zoo

AFP (Oct. 20, 2014) Two white lion cubs, an extremely rare subspecies of the African lion, were recently born at Belgrade Zoo. They are being bottle fed by zoo keepers after they were rejected by their mother after birth. Duration: 00:42 Video provided by AFP
Powered by NewsLook.com
Traditional Farming Methods Gaining Ground in Mali

Traditional Farming Methods Gaining Ground in Mali

AFP (Oct. 20, 2014) He is leading a one man agricultural revolution in Mali - Oumar Diatabe uses traditional farming methods to get the most out of his land and is teaching others across the country how to do the same. Duration: 01:44 Video provided by AFP
Powered by NewsLook.com
Goliath Spider Will Give You Nightmares

Goliath Spider Will Give You Nightmares

Buzz60 (Oct. 20, 2014) An entomologist stumbled upon a South American Goliath Birdeater. With a name like that, you know it's a terrifying creepy crawler. Sean Dowling (@SeanDowlingTV) has the details. Video provided by Buzz60
Powered by NewsLook.com
Adorable Video of Baby Rhino and Lamb Friend Playing

Adorable Video of Baby Rhino and Lamb Friend Playing

Buzz60 (Oct. 20, 2014) Gertjie the Rhino and Lammie the Lamb are teaching the world about animal conservation and friendship. TC Newman (@PurpleTCNewman) has the adorable video! Video provided by Buzz60
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:

Breaking News:

Strange & Offbeat Stories


Plants & Animals

Earth & Climate

Fossils & Ruins

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile: iPhone Android Web
Follow: Facebook Twitter Google+
Subscribe: RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins