Featured Research

from universities, journals, and other organizations

Loss of protective heart failure protein linked to critical limb ischemia

Date:
October 10, 2012
Source:
Thomas Jefferson University
Summary:
Restoring diminished levels of a protein shown to prevent and reverse heart failure damage could also have therapeutic applications for patients with critical limb ischemia (CLI), suggests a new preclinical study.

Restoring diminished levels of a protein shown to prevent and reverse heart failure damage could also have therapeutic applications for patients with critical limb ischemia (CLI), suggests a new preclinical study published online Oct. 9 in Circulation Research from researchers at the Center for Translational Medicine at Thomas Jefferson University.

Low levels of the protein S100A1 have been linked to congestive heart failure and high blood pressure, and now appear to be associated with critical lower limb ischemia, a condition found in type 2 diabetic patients where blood flow to legs is cut off, often resulting in amputation.

Past studies have shown that returning the protein to normal levels or above prevented and/or reversed congestive heart failure in large animal models with gene therapy. Given these new findings, the researchers posit that S100A1 could also be a potential therapeutic target in CLI to promote revascularization.

The team was led by Patrick Most, M.D., adjunct Assistant Professor of Medicine at Thomas Jefferson University, and lead author of the study who now heads the Center for Molecular and Translational Cardiology at the University of Heidelberg, Germany, and Karsten Peppel, Ph.D., an Associate Professor in the Center for Translational Medicine at Jefferson and senior author of the study.

"In patients with CLI, this protein appears to be reduced to extremely low levels, almost non-existent," said Dr. Peppel. "This dovetails with a finding we had when we were looking at patients with heart failure. We already know this protein has an important role in heart muscle cells. In patients who experience heart failure, there was reduced amount of the protein, down to about 40 percent of normal. Here, we showed in mice with CLI a similar defect in vascular cells and the inability to re-vascularize following arterial occlusion."

Without successful revascularization, up to 40 percent of patients with CLI require major limb amputations within a year of diagnosis. CLI has many of the same predisposing risk factors as arteriosclerosis, such as smoking, high blood pressure and Type 2 diabetes. According to the American Diabetes Association, there are over 65,000 lower limb amputations performed in the United States a year as a direct result of diabetes.

"Understanding the fundamentals of disease mechanisms is key to finding better therapies. This actually exceeds the capacity of one laboratory," said Dr. Most. "Our integrative model of sharing expertise and resources between our laboratories in the U.S. and Germany has proven to be quite successful over the last years."

Dr. Most's laboratory has been working on S100A1's role in heart disease for more than a decade, and together with a group from the Center for Translational Medicine at Jefferson, they have proven that loss of the protein causes diseased hearts to fail and that the protein is a potential target for gene therapy for heart failure.

S100A1, part of a larger family of proteins called S100, is primarily found at high levels in muscle, particularly the heart. Falling levels of S100A1 are critical in the loss of heart-pumping strength after a heart attack and play an important role in the progression to heart failure.

Dr. Most and his team have also found that loss of the protective heart failure protein causes high blood pressure.

"The mechanism is based more or less on the availability of nitric oxide," said Dr. Most. "S100A1 also regulates calcium cycling in the endothelial cell, and calcium is needed in the endothelial cell to stimulate nitric oxide production. The loss of S100A1 impairs the calcium mobilization of the endothelial cell -- that's the link between less calcium, less nitric oxide, hypertension and endothelial dysfunction."

"Now, we have shown a down regulation of S100A1 in patients with CLI and identified the protein as critical for endothelial cell function in ischemic angiogenesis," he said. "This is a new therapeutic angle. Perhaps the most intriguing finding is that S100A1 stimulates directly the source enzyme of nitric oxide in endothelial cell. Without S100A1, this mechanism is simply blocked and the body's ability to generate new small vessels severely compromised."

The next step is to determine what causes that down regulation and look for therapeutic development, according to the researchers, adding that they will take advantage of the unique expertise and experience in developing molecular-targeted therapies at the Center.

"Our goal is to determine why these levels drop in patients with peripheral arterial occlusive disease, and how we can get them back up, and if restoration of this protein makes it better," said Dr. Peppel. "There is potential with therapeutics, mainly gene therapy, to restore levels and possibly improve revascularization, not only in patients with CLI but also in heart disease due to insufficient blood supply."


Story Source:

The above story is based on materials provided by Thomas Jefferson University. Note: Materials may be edited for content and length.


Journal Reference:

  1. P. Most, C. Lerchenmuller, G. Rengo, A. Mahlmann, J. Ritterhoff, D. Rohde, C. Goodman, C. J. Busch, F. Laube, J. Heissenberg, S. T. Pleger, N. Weiss, H. A. Katus, W. J. Koch, K. Peppel. S100A1 Deficiency Impairs Post-Ischemic Angiogenesis via Compromised Proangiogenic Endothelial Cell Function and Nitric Oxide Synthase Regulation. Circulation Research, 2012; DOI: 10.1161/CIRCRESAHA.112.275156

Cite This Page:

Thomas Jefferson University. "Loss of protective heart failure protein linked to critical limb ischemia." ScienceDaily. ScienceDaily, 10 October 2012. <www.sciencedaily.com/releases/2012/10/121010101908.htm>.
Thomas Jefferson University. (2012, October 10). Loss of protective heart failure protein linked to critical limb ischemia. ScienceDaily. Retrieved July 31, 2014 from www.sciencedaily.com/releases/2012/10/121010101908.htm
Thomas Jefferson University. "Loss of protective heart failure protein linked to critical limb ischemia." ScienceDaily. www.sciencedaily.com/releases/2012/10/121010101908.htm (accessed July 31, 2014).

Share This




More Health & Medicine News

Thursday, July 31, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Health Insurers' Profits Slide

Health Insurers' Profits Slide

Reuters - Business Video Online (July 30, 2014) Obamacare-related costs were said to be behind the profit plunge at Wellpoint and Humana, but Wellpoint sees the new exchanges boosting its earnings for the full year. Fred Katayama reports. Video provided by Reuters
Powered by NewsLook.com
Concern Grows Over Worsening Ebola Crisis

Concern Grows Over Worsening Ebola Crisis

AFP (July 30, 2014) Pan-African airline ASKY has suspended all flights to and from the capitals of Liberia and Sierra Leone amid the worsening Ebola health crisis, which has so far caused 672 deaths in Guinea, Liberia and Sierra Leone. Duration: 00:43 Video provided by AFP
Powered by NewsLook.com
At Least 20 Chikungunya Cases in New Jersey

At Least 20 Chikungunya Cases in New Jersey

AP (July 30, 2014) At least 20 New Jersey residents have tested positive for chikungunya, a mosquito-borne virus that has spread through the Caribbean. (July 30) Video provided by AP
Powered by NewsLook.com
Xtreme Eating: Your Daily Caloric Intake All On One Plate

Xtreme Eating: Your Daily Caloric Intake All On One Plate

Newsy (July 30, 2014) The Center for Science in the Public Interest released its 2014 list of single meals with whopping calorie counts. Video provided by Newsy
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:

Breaking News:
from the past week

In Other News

... from NewsDaily.com

Science News

Health News

    Environment News

    Technology News



    Save/Print:
    Share:

    Free Subscriptions


    Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

    Get Social & Mobile


    Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

    Have Feedback?


    Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
    Mobile: iPhone Android Web
    Follow: Facebook Twitter Google+
    Subscribe: RSS Feeds Email Newsletters
    Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins