Featured Research

from universities, journals, and other organizations

Injection-free vaccination technique could address global vaccine challenge for HIV, malaria

Date:
February 4, 2013
Source:
King's College London
Summary:
Scientists have demonstrated the ability to deliver a dried live vaccine to the skin without a traditional needle, and shown for the first time that this technique is powerful enough to enable specialized immune cells in the skin to kick-start the immunizing properties of the vaccine.

Scientists at King's have demonstrated the ability to deliver a dried live vaccine to the skin without a traditional needle, and shown for the first time that this technique is powerful enough to enable specialised immune cells in the skin to kick-start the immunising properties of the vaccine.
Credit: Image courtesy of King's College London

Scientists at King's College London have demonstrated the ability to deliver a dried live vaccine to the skin without a traditional needle, and shown for the first time that this technique is powerful enough to enable specialised immune cells in the skin to kick-start the immunising properties of the vaccine.

Funded by the Bill & Melinda Gates Foundation and published today in Proceedings of the National Academy of Sciences, researchers say although it is an early study this important technical advance offers a potential solution to the challenges of delivering live vaccines in resource-limited countries globally, without the need for refrigeration. A cheaper alternative to hypodermic needles, it would also remove safety risks from needle contamination and the pain-free administration could lead to more people taking up a vaccination. The researchers add that it could have an impact beyond infectious disease vaccination programmes, for example managing autoimmune and inflammatory conditions such as diabetes.

HIV, malaria and TB represent major global health challenges. Although promising research is underway to develop vaccines for these diseases, considerable stumbling blocks remain for countries where transporting and storing live vaccines in a continuously cold environment (around 2C to 8C or below) would not be possible. If a cold chain cannot be maintained for a live vaccine there is a high risk it could become unsafe and lose effectiveness.

The team at King's used a silicone mould developed by US company TheraJect to create a microneedle array -- a tiny disc with several micro-needles made of sugar which dissolve when inserted into the skin. The team formulated a dried version of a live modified adenovirus-based candidate HIV vaccine in sugar (sucrose) and used the mould to create the microneedle array. They found that the dried live vaccine remained stable and effective at room temperature.

To test the effectiveness of the microneedle array, they applied it to mice. Using imaging (in collaboration with Professor Frederic Geissmann, King's College London) they observed how the vaccine dissolved in the skin and were able to identify for the first time exactly which specialised immune cells in the skin 'pick up' this type of vaccine and activate the immune system. The researchers found the first evidence that a sub-set of specialised dendritic cells in the skin were responsible for triggering this immune response.

When compared with a traditional needle vaccine method, the immune response generated by the dried microneedle vaccine (kept at room temperature) was equivalent to that induced by the same dose of injected liquid vaccine that had been preserved at -80C.

Dr Linda Klavinskis from the Peter Gorer Department of Immunobiology at King's College London, said: 'We have shown that it is possible to maintain the effectiveness of a live vaccine by drying it in sugar and applying it to the skin using microneedles -- a potentially painless alternative to hypodermic needles. We have also uncovered the role of specific cells in the skin which act as a surveillance system, picking up the vaccine by this delivery system and kick-starting the body's immune processes.

'This work opens up the exciting possibility of being able to deliver live vaccines in a global context, without the need for refrigeration. It could potentially reduce the cost of manufacturing and transportation, improve safety (as there would be no loss in potency), and avoids the need of hypodermic needle injection, reducing the risk of transmitting blood-borne disease from contaminated needles and syringes.

'This new technique represents a huge leap forward in overcoming the challenges of delivering a vaccination programme for diseases such as HIV and malaria. But these findings may also have wider implications for other infectious disease vaccination programmes, for example infant vaccinations, or even other inflammatory and autoimmune conditions such as diabetes.'

The published study from King's College London is part of a larger project funded by the Bill & Melinda Gates Foundation linking other groups, including those at Imperial College London and Royal Holloway University of London, who are working on other aspects of HIV vaccination.


Story Source:

The above story is based on materials provided by King's College London. Note: Materials may be edited for content and length.


Journal Reference:

  1. Veronique Bachy, Catherine Hervouet, Pablo D. Becker, Laurent Chorro, Leo M. Carlin, Shanthi Herath, Timos Papagatsias, Jean-Baptiste Barbaroux, Sea-Jin Oh, Adel Benlahrech, Takis Athanasopoulos, George Dickson, Steven Patterson, Sung-Yun Kwon, Frederic Geissmann, and Linda S. Klavinskis. Langerin negative dendritic cells promote potent CD8 T-cell priming by skin delivery of live adenovirus vaccine microneedle arrays. Proceedings of the National Academy of Sciences, 2013; DOI: 10.1073/pnas.1214449110

Cite This Page:

King's College London. "Injection-free vaccination technique could address global vaccine challenge for HIV, malaria." ScienceDaily. ScienceDaily, 4 February 2013. <www.sciencedaily.com/releases/2013/02/130204153908.htm>.
King's College London. (2013, February 4). Injection-free vaccination technique could address global vaccine challenge for HIV, malaria. ScienceDaily. Retrieved September 17, 2014 from www.sciencedaily.com/releases/2013/02/130204153908.htm
King's College London. "Injection-free vaccination technique could address global vaccine challenge for HIV, malaria." ScienceDaily. www.sciencedaily.com/releases/2013/02/130204153908.htm (accessed September 17, 2014).

Share This



More Health & Medicine News

Wednesday, September 17, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

President To Send 3,000 Military Personnel To Fight Ebola

President To Send 3,000 Military Personnel To Fight Ebola

Newsy (Sep. 16, 2014) President Obama is expected to send 3,000 troops to West Africa as part of the effort to contain Ebola's spread. Video provided by Newsy
Powered by NewsLook.com
Obama Orders Military Response to Ebola

Obama Orders Military Response to Ebola

AP (Sep. 16, 2014) Calling the Ebola outbreak in West Africa a potential threat to global security, President Barack Obama is ordering 3,000 U.S. military personnel to the stricken region amid worries that the outbreak is spiraling out of control. (Sept. 16) Video provided by AP
Powered by NewsLook.com
UN: 20,000 Could Be Infected With Ebola by Year End

UN: 20,000 Could Be Infected With Ebola by Year End

AFP (Sep. 16, 2014) Nearly $1.0 billion dollars is needed to fight the Ebola outbreak raging in west Africa, the United Nations say, warning that 20,000 could be infected by year end. Duration: 00:40 Video provided by AFP
Powered by NewsLook.com
Obama: Ebola Outbreak Threat to Global Security

Obama: Ebola Outbreak Threat to Global Security

AP (Sep. 16, 2014) President Obama is ordering U.S. military personnel to West Africa to deal with the Ebola outbreak, which is he calls a potential threat to global security. (Sept. 16) Video provided by AP
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:

Breaking News:
from the past week

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

    Technology News



    Save/Print:
    Share:

    Free Subscriptions


    Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

    Get Social & Mobile


    Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

    Have Feedback?


    Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
    Mobile: iPhone Android Web
    Follow: Facebook Twitter Google+
    Subscribe: RSS Feeds Email Newsletters
    Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins