Featured Research

from universities, journals, and other organizations

Reprogramming adult cells to stem cells works better with one gene turned off

Date:
March 1, 2013
Source:
Methodist Hospital, Houston
Summary:
The removal of a genetic roadblock could improve the efficiency of converting adult cells into stem cells by 10 to 30 times, report scientists.

Wang and his team are the first to identify Jmjd3's role in inhibiting the reprogramming process. They found knockdown or deletion of Jmjd3 in young mouse fibroblasts was enough to greatly enhance reprogramming efficiency.
Credit: Image courtesy of Methodist Hospital, Houston

The removal of a genetic roadblock could improve the efficiency of converting adult cells into stem cells by 10 to 30 times, report scientists from The Methodist Hospital Research Institute and two other institutions in the latest issue of Cell.

"The discovery six years ago that scientists can convert adult cells into inducible pluripotent stem cells, or iPSCs, bolstered the dream that a patient's own cells might be reprogrammed to make patient-specific iPSCs for regenerative medicine, modeling human diseases in petri dishes, and drug screening," said Rongfu Wang, Ph.D., Principal Investigator and Director of the Center for Inflammation and Epigenetics. "But reprogramming efficiency has remained very low, impeding its applications in the clinic."

Wang and his group identified a protein encoded by the gene Jmjd3 (also called KDM6B) as a roadblock in the stem cell conversion process. Jmjd3 is known to be involved in many biological processes, including the maturation of nerve cells and immune cell differentiation.

Wang and his team are the first to identify Jmjd3's role in inhibiting the reprogramming process. They found knockdown or deletion of Jmjd3 in young mouse fibroblasts was enough to greatly enhance reprogramming efficiency.

"Our findings demonstrate a previously unrecognized role of Jmjd3 in cellular reprogramming and provide molecular insight into the mechanisms by which the Jmjd3-PHF20 axis controls this process," said Helen (Yicheng) Wang, co-principal investigator.

In investigating Jmjd3 and its role in iPSC reprogramming, Wang's team found Jmjd3 has two previously unknown functions -- it helps regulate cell growth and cellular aging and Jmjd3 deactivates another nuclear protein, PHF20. The scientists learned during the study that PHF20 is required for cellular reprogramming, because cells without PHF20 failed to generate iPSCs.

"So when it comes to increasing iPSC yields, knocking down Jmjd3 is like hitting two birds with one stone," Rongfu Wang said.

Jmjd3 may not be the only genetic roadblock to stem cell conversion.

"Removal of multiple roadblocks could further enhance the reprogramming efficiency with which researchers can efficiently generate patient-specific iPSCs for clinical applications," Wang said.


Story Source:

The above story is based on materials provided by Methodist Hospital, Houston. Note: Materials may be edited for content and length.


Journal Reference:

  1. Wei Zhao, Qingtian Li, Stephen Ayers, Yifeng Gu, Zhong Shi, Qingyuan Zhu, Yidong Chen, HelenY. Wang, Rong-Fu Wang. Jmjd3 Inhibits Reprogramming by Upregulating Expression of INK4a/Arf and Targeting PHF20 for Ubiquitination. Cell, 2013; 152 (5): 1037 DOI: 10.1016/j.cell.2013.02.006

Cite This Page:

Methodist Hospital, Houston. "Reprogramming adult cells to stem cells works better with one gene turned off." ScienceDaily. ScienceDaily, 1 March 2013. <www.sciencedaily.com/releases/2013/03/130301153247.htm>.
Methodist Hospital, Houston. (2013, March 1). Reprogramming adult cells to stem cells works better with one gene turned off. ScienceDaily. Retrieved October 21, 2014 from www.sciencedaily.com/releases/2013/03/130301153247.htm
Methodist Hospital, Houston. "Reprogramming adult cells to stem cells works better with one gene turned off." ScienceDaily. www.sciencedaily.com/releases/2013/03/130301153247.htm (accessed October 21, 2014).

Share This



More Health & Medicine News

Tuesday, October 21, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

CDC Revamps Ebola Guidelines After Criticism

CDC Revamps Ebola Guidelines After Criticism

Newsy (Oct. 21, 2014) The Centers for Disease Control and Prevention have issued new protocols for healthcare workers interacting with Ebola patients. Video provided by Newsy
Powered by NewsLook.com
WHO: Ebola Vaccine Trials to Start a in January

WHO: Ebola Vaccine Trials to Start a in January

AP (Oct. 21, 2014) Tens of thousands of doses of experimental Ebola vaccines could be available for "real-world" testing in West Africa as soon as January as long as they are deemed safe in soon to start trials, the World Health Organization said Tuesday. (Oct. 21) Video provided by AP
Powered by NewsLook.com
First-Of-Its-Kind Treatment Gives Man Ability To Walk Again

First-Of-Its-Kind Treatment Gives Man Ability To Walk Again

Newsy (Oct. 21, 2014) A medical team has for the first time given a man the ability to walk again after transplanting cells from his brain onto his severed spinal cord. Video provided by Newsy
Powered by NewsLook.com
CDC Issues New Ebola Guidelines for Health Workers

CDC Issues New Ebola Guidelines for Health Workers

Reuters - US Online Video (Oct. 21, 2014) The U.S. Centers for Disease Control and Prevention has set up new guidelines for health workers taking care of patients infected with Ebola. Linda So reports. Video provided by Reuters
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:

Breaking News:

Strange & Offbeat Stories


Health & Medicine

Mind & Brain

Living & Well

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile: iPhone Android Web
Follow: Facebook Twitter Google+
Subscribe: RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins