A protein known to be a key player in the development of Parkinson's disease is able to enter and harm cells in the same way that viruses do, according to a Loyola University Chicago Stritch School of Medicine study.
The protein is called alpha-synuclein. The study shows how, once inside a neuron, alpha synuclein breaks out of lysosomes, the digestive compartments of the cell. This is similar to how a cold virus enters a cell during infection. The finding eventually could lead to the development of new therapies to delay the onset of Parkinson's disease or halt or slow its progression, researchers said.
The study by virologist Edward Campbell, PhD, and colleagues, was published April 25, 2013 in the journal PLOS ONE.
Alpha-synuclein plays a role in the normal functioning of healthy neurons. But in Parkinson's disease patients, the protein turns bad, aggregating into clumps that lead to the death of neurons in the area of the brain responsible for motor control. Previous studies have shown that these protein aggregates can enter and harm cells. Campbell and colleagues showed how alpha synuclein can bust out of lysosomes, small structures that collectively serve as the cell's digestive system. The rupture of these bubble-like structures, known as vesicles, releases enzymes that are toxic to the rest of the cell.
"The release of lysosomal enzymes is sensed as a 'danger signal' by cells, since similar ruptures are often induced by invading bacteria or viruses," said Chris Wiethoff, a collaborator on the study. "Lysosomes are often described as 'suicide bags' because when they are ruptured by viruses or bacteria, they induce oxidative stress that often leads to the death of the affected cell."
In a viral or bacterial infection, the deaths of such infected cells may overall be a good thing for the infected individual. But in Parkinson's disease, this same protective mechanism may lead to the death of neurons and enhance the spread of alpha-synuclein between cells in the brain, Campbell said. "This might explain the progressive nature of Parkinson's disease. More affected cells leads to the spread of more toxic alpha-synuclein aggregates in the brain," Campbell said. "This is very similar to what happens in a spreading viral infection."
Campbell stressed that these studies need to be followed up and confirmed in other models of Parkinson's disease. "Using cultured cells, we have made some exciting observations. However, we need to understand how lysosomal rupture is affecting disease progression in animal models of Parkinson's disease and, ultimately, the brains of people affected by Parkinson's disease. Can we interfere with the ability of alpha-synuclein to rupture lysosomes in these settings? And will that have a positive effect on disease progression? These are the questions we are excited to be asking next."
Jeffrey H. Kordower, PhD, professor of neurological sciences, professor of neurosurgery and director of the Research Center for Brain Repair at Rush University Medical Center, said the study "is an important finding by a group of investigators who are beginning to make their impact in the field of Parkinson's disease. This paper adds to the growing concept that alpha-synuclein, a main culprit in the cause of Parkinson's disease, can transfer from cell to cell. This paper elegantly puts a mechanism behind such a transfer. The findings will help shape the direction of Parkinson's disease research for years to come."
Campbell and Wiethoff are assistant professors in the Department of Microbiology and Immunology at Loyola University Chicago Stritch School of Medicine. Other co-authors are David Freeman (first author), Rudy Cedillos, Samantha Choyke, Zana Lukic, Kathleen McGuire, Shauna Marvin, Andrew M. Burrage and Ajay Rana of Loyola's Stritch School of Medicine; Stacey Sudholt of Missouri School of Medicine; and Christopher O'Connor of North Central College in Naperville, Il.
- David Freeman, Rudy Cedillos, Samantha Choyke, Zana Lukic, Kathleen McGuire, Shauna Marvin, Andrew M. Burrage, Stacey Sudholt, Ajay Rana, Christopher O'Connor, Christopher M. Wiethoff, Edward M. Campbell. Alpha-Synuclein Induces Lysosomal Rupture and Cathepsin Dependent Reactive Oxygen Species Following Endocytosis. PLoS ONE, 2013; 8 (4): e62143 DOI: 10.1371/journal.pone.0062143
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