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Researchers use nanoscale 'patches' to sensitize targeted cell receptors

Date:
November 25, 2013
Source:
North Carolina State University
Summary:
Researchers have developed nanoscale 'patches' that can be used to sensitize targeted cell receptors, making them more responsive to signals that control cell activity. The finding holds promise for promoting healing and facilitating tissue engineering research.
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Researchers from North Carolina State University and Duke University have developed nanoscale "patches" that can be used to sensitize targeted cell receptors, making them more responsive to signals that control cell activity. The finding holds promise for promoting healing and facilitating tissue engineering research.

The research takes advantage of the fact that cells in a living organism can communicate via physical contact. Specifically, when targeted receptors on the surface of a cell are triggered, the cell receives instructions to alter its behavior in some way. For example, the instructions may cause a stem cell to differentiate into a bone cell or a cartilage cell.

These receptors respond to specific ligands, or target molecules. And those ligands have to be present in certain concentrations in order to trigger the receptors. If there aren't enough target ligands, the receptors won't respond.

Now researchers have developed nanoscale patches that are embedded with tiny protein fragments called peptides. These peptides bond to a specific cell receptor, making it more sensitive to its target ligand -- meaning that it takes fewer ligand molecules to trigger the receptor and its resulting behavior modification.

"This study shows that our concept can work, and there are a host of potential applications," says Dr. Thom LaBean, an associate professor of materials science at NC State and senior author of a paper describing the work. "For example, if we identify the relevant peptides, we could create patches that sensitize cells to promote cartilage growth on one side of the patch and bone growth on the other side. This could be used to expedite healing or to enable tissue engineering of biomedical implants."

"What's important about this is that it allows us to be extremely precise in controlling cell behavior and gene expression," says Ronnie Pedersen, a Ph.D. student at Duke University and lead author of the paper. "By controlling which peptides are on the patch, we can influence the cell's activity. And by manipulating the placement of the patch, we can control where that activity takes place."

The patch itself is made of DNA that researchers have programmed to self-assemble into flexible, two-dimensional sheets. The sheets themselves incorporate molecules called biotin and streptavidin which serve to hold and organize the peptides that are used to sensitize cell receptors.

"These peptides can bind with cell receptors and sensitize them, without blocking the interaction between the receptors and their target ligands," Pedersen says. "That's what makes this approach work."


Story Source:

The above post is reprinted from materials provided by North Carolina State University. Note: Materials may be edited for content and length.


Journal Reference:

  1. Ronnie O. Pedersen, Elizabeth G. Loboa, Thomas H. LaBean. Sensitization of Transforming Growth Factor-β Signaling by Multiple Peptides Patterned on DNA Nanostructures. Biomacromolecules, 2013; 131108152109000 DOI: 10.1021/bm4011722

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North Carolina State University. "Researchers use nanoscale 'patches' to sensitize targeted cell receptors." ScienceDaily. ScienceDaily, 25 November 2013. <www.sciencedaily.com/releases/2013/11/131125121755.htm>.
North Carolina State University. (2013, November 25). Researchers use nanoscale 'patches' to sensitize targeted cell receptors. ScienceDaily. Retrieved July 31, 2015 from www.sciencedaily.com/releases/2013/11/131125121755.htm
North Carolina State University. "Researchers use nanoscale 'patches' to sensitize targeted cell receptors." ScienceDaily. www.sciencedaily.com/releases/2013/11/131125121755.htm (accessed July 31, 2015).

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