Sanford-Burnham scientists have identified the B and T Lymphocyte Attenuator (BTLA) inhibitory receptor as a key factor in limiting inflammatory responses, particularly in the skin. The study, published online today in Immunity, provides clarity on how T cells get fired up to protect against pathogens, and then cool down to restore immune homeostasis.
"Our study shows that BTLA expression in gamma-delta T-cells deactivates their response to immune stimuli," said Carl Ware, Ph.D., professor and director of the Infectious and Inflammatory Disease Center at Sanford-Burnham. "Gamma-delta T-cells are the first line of defense against pathogens -- and unless 'turned off', can lead to unwanted inflammation and tissue destruction."
Until now, scientists knew that gamma-delta T-cells were important for initiating inflammatory responses in the skin, but not how to turn off these potent cells.
"Now, we know that BTLA acts as a critical coordinator for turning T cells off to prevent the immune system from spinning out of control, and helping to rebalance the immune system," said Ware.
The findings could help scientists develop new treatments for inflammatory disorders by targeting BTLA to reduce inflammation, promote homeostasis, and control disease.
How BTLA is regulated
Using a combination of human cells and a mouse model of psoriasis, the research team described a new pathway that regulates BTLA expression. Ware's research showed that the "retinoid-related orphan receptor gamma-t" (ROR gamma-t) nuclear transcription factor works with interleukin (IL)-7, to coordinate the expression of BTLA, which in turn regulates gamma-delta T cell responses to inflammatory stimuli.
The study found that ROR gamma-t works to inhibit BTLA transcription, thereby limiting its availability in gamma-delta T-cells. This allows the expansion of T-cell numbers and their production of inflammatory cytokines, including IL-17 and TNF.
In contrast, IL-7 increases the availability of BTLA on the cell surface, reducing the number of active T cells and allowing BTLA to rein in the immune response.
"To be effective against pathogens, yet prevent damage from the body's own defenses, the immune system has to maintain a balance. In essence, BTLA helps control inflammatory responses by reducing the activity and numbers of active gamma-delta T cells," said Ware.
Immune mediated inflammatory diseases (IMIDs)
IMIDs are chronic, often disabling diseases caused by cytokine dysregulation and inflammation. There are over 80 types of IMIDs that target virtually any part of the body, including skin, connective tissue, and respiratory and gastrointestinal systems. Common IMIDs include Crohn's disease, ulcerative colitis, psoriasis, rheumatoid arthritis and lupus. Approximately 5-7% of Western society suffers from an IMID that requires treatment.
Current treatments for IMIDs include corticosteroids, immuno-suppressants and "biologics" that target specific immune signaling molecules. While these therapies are very effective in some patients, many patients have a poor response to these drugs.
"Understanding the mechanisms that control immune responses creates important breakthroughs for researchers developing drugs to treat these chronic diseases. If a drug can selectively activate BTLA, we put the brakes on gamma-delta T-cells and gain control of inflammation, prevent damage, and if possible, achieve long-term disease remission," said Ware.
- Vasileios Bekiaris, John R. Šedý, Matthew G. Macauley, Antje Rhode-Kurnow, Carl F. Ware. The Inhibitory Receptor BTLA Controls γδ T Cell Homeostasis and Inflammatory Responses. Immunity, 2013; DOI: 10.1016/j.immuni.2013.10.017
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