Featured Research

from universities, journals, and other organizations

Genomic tumor testing conducted

Date:
January 22, 2014
Source:
Norris Cotton Cancer CenterDartmouth-Hitchcock Medical Center
Summary:
Clinical laboratory experts and physicians achieved 100 percent accuracy using new gene sequencing equipment and panels to test for abnormal DNA in cancerous tumor cells, paving the way for routine genetic testing in personalizing cancer care

The NCCC Department of Pathology clinical laboratory is now able to perform genomic sequencing tests on patient tumor tissue.
Credit: Norris Cotton Cancer CenterDartmouth-Hitchcock Medical Center

Clinical laboratory experts and physicians at Dartmouth-Hitchcock Norris Cotton Cancer Center (NCCC) achieved 100 percent accuracy in testing for abnormal DNA in cancerous tumor cells with its new gene sequencing equipment and panels. The results, published in the journal Clinical Chemistry and Laboratory Medicine, confirm the precision of the test for routine patient care so physicians can tailor treatment to an individual person's DNA, improving the chances of a successful outcome.

"We evaluated next generation tools for gene sequencing in our clinical laboratory," said Gregory Tsongalis, PhD, director, molecular pathology. "The equipment and approach we are using is faster, more sensitive, and more reliable than previous approaches. It paves the way for routine genetic testing in personalizing cancer care here at the Norris Cotton Cancer Center."

The genomic sequencing test, called the Ion Torrent AmpliSeq Cancer Hotspot Panel, is often used in research settings. Now the clinically certified and accredited pathology laboratory at NCCC has put in place the ability to routinely perform this test on patient tumor tissue. The NCCC Department of Pathology clinical laboratory, where this testing is performed, is in compliance with the Clinical Laboratory Improvement Amendment (CLIA), which certifies laboratories to federal standards of quality, proficiency, and safety. It is also accredited by the College of American Pathologists, the gold standard in laboratory accreditation.

"This takes genetic sequencing information or molecular profiling of tumors and puts it into the hands of physicians treating patients today," said Tsongalis.

The laboratory has run over 400 patient samples, about 80 percent of which have identified genetic mutations. About 50 to 60 percent of the tests identify genetic mutations that change the course of treatment. Turnaround time for the test is one week to 10 days. Physicians and scientists test tumor samples from metastatic colon cancer, melanoma, gliomas, and non-small cell lung cancers. In the months to come, they will add testing for breast cancer, leukemia, and lymphoma. Patients' insurance companies reimburse for testing on known mutations as single-gene tests. The Norris Cotton Cancer laboratory, however, runs each sample against a wide panel of genetic mutations. The Cancer Hotspot Panel tests for mutations in 50 common cancer genes as part of a molecular profiling strategy to personalize therapy for an individual patient.

DNA is supposed to be well ordered, and it is considered damaged or mutated when there is an extra or missing section. Genetic mutations can be inherited or caused by environmental factors such as exposure to sunlight, cigarette smoke, or other carcinogens. Damaged DNA can send out the wrong messages to cells: telling them to multiply and grow in ways that can lead to tumors. By identifying the specific mutations in a tumor, physicians can chose medications that precisely target that location for treatment.


Story Source:

The above story is based on materials provided by Norris Cotton Cancer CenterDartmouth-Hitchcock Medical Center. Note: Materials may be edited for content and length.


Journal Reference:

  1. Gregory J. Tsongalis, Jason D. Peterson, Francine B. de Abreu, Christopher D. Tunkey, Torrey L. Gallagher, Linda D. Strausbaugh, Wendy A. Wells, Christopher I. Amos. Routine use of the Ion Torrent AmpliSeq™ Cancer Hotspot Panel for identification of clinically actionable somatic mutations. Clinical Chemistry and Laboratory Medicine, 2013; 0 (0): 1 DOI: 10.1515/cclm-2013-0883

Cite This Page:

Norris Cotton Cancer CenterDartmouth-Hitchcock Medical Center. "Genomic tumor testing conducted." ScienceDaily. ScienceDaily, 22 January 2014. <www.sciencedaily.com/releases/2014/01/140122153613.htm>.
Norris Cotton Cancer CenterDartmouth-Hitchcock Medical Center. (2014, January 22). Genomic tumor testing conducted. ScienceDaily. Retrieved July 29, 2014 from www.sciencedaily.com/releases/2014/01/140122153613.htm
Norris Cotton Cancer CenterDartmouth-Hitchcock Medical Center. "Genomic tumor testing conducted." ScienceDaily. www.sciencedaily.com/releases/2014/01/140122153613.htm (accessed July 29, 2014).

Share This




More Health & Medicine News

Tuesday, July 29, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Deadly Ebola Virus Threatens West Africa

Deadly Ebola Virus Threatens West Africa

AP (July 28, 2014) West African nations and international health organizations are working to contain the largest Ebola outbreak in history. It's one of the deadliest diseases known to man, but the CDC says it's unlikely to spread in the U.S. (July 28) Video provided by AP
Powered by NewsLook.com
$15B Deal on Vets' Health Care Reached

$15B Deal on Vets' Health Care Reached

AP (July 28, 2014) A bipartisan deal to improve veterans health care would authorize at least $15 billion in emergency spending to fix a veterans program scandalized by long patient wait times and falsified records. (July 28) Video provided by AP
Powered by NewsLook.com
Two Americans Contract Ebola in Liberia

Two Americans Contract Ebola in Liberia

Reuters - US Online Video (July 28, 2014) Two American aid workers in Liberia test positive for Ebola while working to combat the deadliest outbreak of the virus ever. Linda So reports. Video provided by Reuters
Powered by NewsLook.com
Traditional African Dishes Teach Healthy Eating

Traditional African Dishes Teach Healthy Eating

AP (July 28, 2014) Classes are being offered nationwide to encourage African Americans to learn about cooking fresh foods based on traditional African cuisine. The program is trying to combat obesity, heart disease and other ailments often linked to diet. (July 28) Video provided by AP
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:

Breaking News:
from the past week

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile: iPhone Android Web
Follow: Facebook Twitter Google+
Subscribe: RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins