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Small-RNA pathway defends genome against enemy within

Date:
March 16, 2014
Source:
Cold Spring Harbor Laboratory
Summary:
For a plant to create reproductive cells, it must first erase a series of tags attached to DNA across the genome that distinguish active and inactive genes. But the marks also keep a host of damaging 'jumping genes' inactive. As the cell wipes away the marks, it activates transposons, which can cause genetic damage. Researchers have discovered a fail-safe mechanism that helps to keep transposons inactive even when these marks are erased.

"Jumping genes" or transposable elements are long stretches of repetitive DNA that can insert themselves throughout the genome. Normally, they are held inactive by a series of marks along the genome. But when these marks are erased, activated transposons can disrupt critical genes, causing dramatic defects in development. Here are two flowers: on the left is a normal plant where transposons are silent; on the right, transposons have been activated, severely mutating the flower. Researchers at CSHL have discovered a new fail-safe mechanism that prevents transposon activation even when the inactivating marks have been erased.
Credit: Rob Martienssen, Cold Spring Harbor Laboratory

Reproductive cells, such as an egg and sperm, join to form stem cells that can mature into any tissue type. But how do reproductive cells arise? We humans are born with all of the reproductive cells that we will ever produce. But in plants things are very different. They first generate mature, adult cells and only later "reprogram" some of them to produce eggs and sperm.

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For a plant to create reproductive cells, it must first erase a key code, a series of tags attached to DNA across the genome known as epigenetic marks. These marks distinguish active and inactive genes. But the marks serve another critical role. They keep a host of damaging transposons, or "jumping genes," inactive. As the cell wipes away the epigenetic code, it activates transposons, placing the newly formed reproductive cell in great danger of sustaining genetic damage.

Today, researchers at Cold Spring Harbor Laboratory (CSHL) led by Professor and HHMI Investigator Robert Martienssen announce the discovery of a pathway that helps to keep transposons inactive even when the epigenetic code is erased.

"Jumping genes" were first identified more than 50 years ago at CSHL by Nobel-prize winning researcher Barbara McClintock. Subsequent study revealed that jumping genes (or transposable elements) are long, repetitive stretches of DNA. They resemble remnants of ancient viruses that have inserted themselves into their host DNA. When active, transposons copy themselves and jump around in the genome. They can insert themselves right in the middle of genes, thus interrupting them. Scientists have found that more than 50% of the human genome is made up of transposons. Remarkably, in plants, up to 90% of the genome is composed of these repetitive sequences.

When a transposon is activated, it can insert itself within critical genes, disrupting gene function and causing infertility and many diseases. To combat this ever present threat from within, the cell has devised stringent mechanisms to maintain tight control over transposon activity. The primary mechanism is the epigenetic code, a kind of secondary layer of genetic information that determines how our DNA is used. Epigenetic marks decorate human DNA, delineating active and inactive genes. Regions of the genome that are rich in transposons are heavily marked with inactivating signals, which silence transposons.

The problem for plants, in particular, is that some cells eliminate nearly all epigenetic marks during reproduction. "The loss of these marks puts the cell in tremendous danger, especially at critical times like reproduction," says CSHL postdoctoral fellow Kate Creasey, Ph.D., lead author on the paper appearing in Nature. "There must be another mechanism to prevent this kind of widespread genomic disruption."

The CSHL team discovered a pathway that does precisely this. The pathway they describe acts as a fail-safe to prevent transposon damage when epigenetic silencing is lost. The cell uses small RNAs, known as microRNAs, to accomplish the task. MicroRNAs were already known to regulate gene expression during development. "Now we show that microRNAs actually target transposons when they are activated, for example in the germline (or reproductive cells)," says Martienssen. "This hints that they may have evolved as a transposon defense mechanism."

Working in collaboration with Professor Blake Meyers at the University of Delaware, Martienssen and his colleagues found that microRNAs silence transposons through a new class of small RNAs known as easiRNAs. Animals have a similar transposon defense mechanism, says Martienssen. "The pathway we have discovered parallels with small RNA systems in animal germlines (called piwi-interacting RNAs, or piRNAs) which also protect against transposons when the genome is being reprogrammed."


Story Source:

The above story is based on materials provided by Cold Spring Harbor Laboratory. Note: Materials may be edited for content and length.


Journal Reference:

  1. Kate M. Creasey, Jixian Zhai, Filipe Borges, Frederic Van Ex, Michael Regulski, Blake C. Meyers & Robert A. Martienssen. miRNAs trigger widespread epigenetically activated siRNAs from transposons in Arabidopsis. Nature, March 2014 DOI: 10.1038/nature13069

Cite This Page:

Cold Spring Harbor Laboratory. "Small-RNA pathway defends genome against enemy within." ScienceDaily. ScienceDaily, 16 March 2014. <www.sciencedaily.com/releases/2014/03/140316153320.htm>.
Cold Spring Harbor Laboratory. (2014, March 16). Small-RNA pathway defends genome against enemy within. ScienceDaily. Retrieved December 19, 2014 from www.sciencedaily.com/releases/2014/03/140316153320.htm
Cold Spring Harbor Laboratory. "Small-RNA pathway defends genome against enemy within." ScienceDaily. www.sciencedaily.com/releases/2014/03/140316153320.htm (accessed December 19, 2014).

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