Featured Research

from universities, journals, and other organizations

Ultrathin collagen matrix biomaterial tool for 3-D microtissue engineering

Date:
April 3, 2014
Source:
World Scientific
Summary:
A novel ultrathin collagen matrix assembly allows for the unprecedented maintenance of liver cell morphology and function in a microscale 'organ-on-a-chip' device that is one example of 3D microtissue engineering. This technology allows researchers to provide cells with the precise extracellular matrix cues that they require to maintain their differentiated form and liverspecific functions, including albumin and urea production.

An ultrathin collagen matrix assembly maintained the morphology and function of primary liver hepatocytes in a microfluidic organ-on-a-chip device for two weeks. Three images of the hepatocytes after two weeks in a microfluidic device: A phase contrast image of cell morphology at two weeks showing dense cyto-plasm, distinct nuclei, and bright cell borders; bile canalicular network develop-ment (red); and cell nuclei (blue) and actin (green) organization demonstrating cell polarity. Scale bar: 50 microns.
Credit: William McCarty, the Center for Engineering in Medicine at the Massachusetts General Hospital, Harvard Medi-cal School, and the Shriners Hospitals for Children-Boston

A novel ultrathin collagen matrix assembly allows for the unprecedented maintenance of liver cell morphology and function in a microscale "organ-on-a-chip" device that is one example of 3-D microtissue engineering.

A team of researchers from the Center for Engineering in Medicine at the Massachusetts General Hospital have demonstrated a new nanoscale matrix biomaterial assembly that can maintain liver cell morphology and function in microfluidic devices for longer times than has been previously been reported in microfluidic devices. This technology allows researchers to provide cells with the precise extracellular matrix cues that they require to maintain their differentiated form and liverspecific functions, including albumin and urea production. The novel technique reported offers a new tool for basic science and pre-clinical investigations, and allows for the creation of stable liver microtissues for use in organ-on-a-chip devices to mimic healthy liver physiology, investigate liver diseases, and test the toxicity of potential therapeutic drugs before using animals or clinical studies. This report appears in the current issue of the journal Technology.

"This is a clever combination of the well-known layer-by-layer deposition technique for creating thin matrix assemblies and collagen functionalization chemistries that will really enable complex liver microtissue engineering by replicating the physiological cues that maintain the state of liver cell differentiation," says Martin Yarmush, M.D., Ph.D., of the Massachusetts General Hospital and senior author on this paper. "The ultrathin collagen matrix biomaterial and its ability to keep liver cells functional for longer periods of time in chip devices will undoubtedly be a useful tool for creating liver microtissues that mimic the true physiology of the liver, including cell and matrix spatial geometries." By creating polyanionic and polycationic solutions of collagen, a ubiquitous extracellular matrix molecule, and alternately exposing liver cells seeded in microfluidic devices to the solutions, the investigators were able to create a nanoscale assembly of collagen on top of the cells. This process can be used to form biologically relevant coatings on many types of charged surfaces to direct cell alignment, increase attachment efficiency, and maintain morphology and function.

"This technique is a nice example of how the translation of established methods for cell culture into microfluidic devices can produce new tools for understanding biological systems, such as cell-matrix interactions," says William McCarty, Ph.D., the lead author on this paper. The team from the Massachusetts General Hospital plans to use this technology in a variety of engineering applications, including constructing liver microtissues by layering together the different types of cells that make up the liver. The liver plays a central role in human-drug interactions and is a common target for drug-induced toxicity, which can result in costly, late-stage and post-approval drug failures when animal models fail to predict human toxicity reactions. To address the current lack of predictive in vitro tools, the investigators are developing scalable liver micro-tissues that could be used to better understand the toxic effects of drugs, as well as provide a high throughput system for testing drug-drug interactions.


Story Source:

The above story is based on materials provided by World Scientific. Note: Materials may be edited for content and length.


Journal Reference:

  1. C.S. Tsai, R.W. Mao, S.K. Lin, Y. Zhu, S.C. Tsai. Faraday instability-based micro droplet ejection for inhalation drug delivery. TECHNOLOGY, 2014; 02 (01): 75 DOI: 10.1142/S233954781450006X

Cite This Page:

World Scientific. "Ultrathin collagen matrix biomaterial tool for 3-D microtissue engineering." ScienceDaily. ScienceDaily, 3 April 2014. <www.sciencedaily.com/releases/2014/04/140403095407.htm>.
World Scientific. (2014, April 3). Ultrathin collagen matrix biomaterial tool for 3-D microtissue engineering. ScienceDaily. Retrieved July 28, 2014 from www.sciencedaily.com/releases/2014/04/140403095407.htm
World Scientific. "Ultrathin collagen matrix biomaterial tool for 3-D microtissue engineering." ScienceDaily. www.sciencedaily.com/releases/2014/04/140403095407.htm (accessed July 28, 2014).

Share This




More Health & Medicine News

Monday, July 28, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Traditional African Dishes Teach Healthy Eating

Traditional African Dishes Teach Healthy Eating

AP (July 28, 2014) Classes are being offered nationwide to encourage African Americans to learn about cooking fresh foods based on traditional African cuisine. The program is trying to combat obesity, heart disease and other ailments often linked to diet. (July 28) Video provided by AP
Powered by NewsLook.com
West Africa Gripped by Deadly Ebola Outbreak

West Africa Gripped by Deadly Ebola Outbreak

AFP (July 28, 2014) The worst-ever outbreak of the deadly Ebola epidemic grips west Africa, killing hundreds. Duration: 00:48 Video provided by AFP
Powered by NewsLook.com
Trees Could Save More Than 850 Lives Each Year

Trees Could Save More Than 850 Lives Each Year

Newsy (July 27, 2014) A national study conducted by the USDA Forest Service found that trees collectively save more than 850 lives on an annual basis. Video provided by Newsy
Powered by NewsLook.com
Google's Next Frontier: The Human Body

Google's Next Frontier: The Human Body

Newsy (July 27, 2014) Google is collecting genetic and molecular information to paint a picture of the perfectly healthy human. Video provided by Newsy
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:

Breaking News:
from the past week

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile: iPhone Android Web
Follow: Facebook Twitter Google+
Subscribe: RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins