Featured Research

from universities, journals, and other organizations

Reversing Heart Failure: Cold Virus Could Make Gene Therapy Possible

Date:
September 29, 1997
Source:
University Of Maryland, Baltimore
Summary:
University of Maryland researchers have confirmed the link between a calcium-handling enzyme and heart-beat strength. They also have enhanced the heart's beating strength using a genetically altered adenovirus to give heart muscle cells extra copies of the gene for the crucial enzyme.

University of Maryland researchers have confirmed the link between a calcium-handling enzyme and the strength of the heart’s beat. They also have been able to enhance the heart’s beating strength, using a genetically altered adenovirus (the virus that causes colds) to give heart muscle cells additional copies of the gene that produces the crucial enzyme.

Their research could pave the way for gene therapy in certain kinds of heart failure, repealing a death sentence for many. Cardiovascular diseases are the leading cause of death in the population as a whole.

Dr. Guiseppe Inesi and Dr. Terry Rogers will present their findings to a New York Academy of Sciences meeting on September 29 at the Hyatt Regency on Capitol Hill in Washington, DC. Inesi is professor and chairman of the Department of Biochemistry and Molecular Biology at the University of Maryland School of Medicine, where Rogers is a professor of biochemistry and molecular biology.

During the four-day meeting, scientists from a variety of disciplines will review progress in characterizing the underlying mechanisms of heart failure, examine unresolved questions, and discuss potential therapies.

The Maryland researchers made their discovery from two complementary experiments. Using thapsigargin—a substance extracted from a Mediterranean plant—to block the enzyme ATPase (adenosine triphosphatase) in heart muscle cells, Inesi and colleagues caused a marked weakening of contractions. Since ATPase is an enzyme that regulates the flow of positively charged calcium ions through heart muscle cells, the experiment showed that the calcium movements sustained by this enzyme are vital to the development of a strong heart beat.

"We observed a marked reduction of intracellular calcium ion transients and a negative effect on muscle contraction, demonstrating the prominent role of the calcium-handling ATPase in the contraction-relaxation cycle," Inesi said.

In a related experiment, the Maryland researchers altered an adenovirus so that it can’t reproduce. Then, using recombinant DNA techniques, they turned it into an efficient vehicle for transporting additional copies of the ATPase gene into heart muscle cells. This gene-copy enrichment resulted in a marked increase in the ability of the cells to control intracellular movements of calcium ions and to develop contractile strength—a strong heart beat.

"The complementary experiments with thapsigargin and recombinant adenovirus demonstrate that the calcium-handling ATPase is a crucial enzyme in regulation of contractile strength in heart muscle," said Inesi. "The conceptual understanding and the techniques established by this work are an important step in the possible development of gene therapy for certain types of heart failure."

The research was funded by the National Institutes of Health.


Story Source:

The above story is based on materials provided by University Of Maryland, Baltimore. Note: Materials may be edited for content and length.


Cite This Page:

University Of Maryland, Baltimore. "Reversing Heart Failure: Cold Virus Could Make Gene Therapy Possible." ScienceDaily. ScienceDaily, 29 September 1997. <www.sciencedaily.com/releases/1997/09/970925133909.htm>.
University Of Maryland, Baltimore. (1997, September 29). Reversing Heart Failure: Cold Virus Could Make Gene Therapy Possible. ScienceDaily. Retrieved September 2, 2014 from www.sciencedaily.com/releases/1997/09/970925133909.htm
University Of Maryland, Baltimore. "Reversing Heart Failure: Cold Virus Could Make Gene Therapy Possible." ScienceDaily. www.sciencedaily.com/releases/1997/09/970925133909.htm (accessed September 2, 2014).

Share This




More Health & Medicine News

Tuesday, September 2, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

U.N. Says Ebola Travel Restrictions Will Cause Food Shortage

U.N. Says Ebola Travel Restrictions Will Cause Food Shortage

Newsy (Sep. 2, 2014) The U.N. says the problem is two-fold — quarantine zones and travel restrictions are limiting the movement of both people and food. Video provided by Newsy
Powered by NewsLook.com
Get on Your Bike! London Cycling Popularity Soars Despite Danger

Get on Your Bike! London Cycling Popularity Soars Despite Danger

AFP (Sep. 1, 2014) Wedged between buses, lorries and cars, cycling in London isn't for the faint hearted. Nevertheless the number of people choosing to bike in the British capital has doubled over the past 15 years. Duration: 02:27 Video provided by AFP
Powered by NewsLook.com
Can You Train Your Brain To Eat Healthy?

Can You Train Your Brain To Eat Healthy?

Newsy (Sep. 1, 2014) New research says if you condition yourself to eat healthy foods, eventually you'll crave them instead of junk food. Video provided by Newsy
Powered by NewsLook.com
We've Got Mites Living In Our Faces And So Do You

We've Got Mites Living In Our Faces And So Do You

Newsy (Aug. 30, 2014) A new study suggests 100 percent of adult humans (those over 18 years of age) have Demodex mites living in their faces. Video provided by Newsy
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:

Breaking News:
from the past week

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile: iPhone Android Web
Follow: Facebook Twitter Google+
Subscribe: RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins