Featured Research

from universities, journals, and other organizations

Viral Vector Delivers Dystrophin Gene To Mouse Muscle

Date:
November 4, 1998
Source:
University Of Michigan
Summary:
University of Michigan scientists have developed a new generation of "gutted" viral vectors that deliver the gene for dystrophin to the muscles of adult mice with muscular dystrophy without triggering their immune systems to attack the foreign virus.

DENVER---University of Michigan scientists have developed a new generation of "gutted" viral vectors that deliver the gene for dystrophin to the muscles of adult mice with muscular dystrophy without triggering their immune systems to attack the foreign virus. The vector is called a "gutted" virus, because it has been stripped of most of its original genes to make room for the large dystrophin gene.

At last year's American Society for Human Genetics meeting, Jeffrey S. Chamberlain, Ph.D., associate professor of human genetics in the U-M Medical School, reported the vector's ability to induce long-term expression of the full-sized dystrophin protein in an immuno-deficient strain of adult mice with Duchenne muscular dystrophy. At this year's meeting, Giovanni Salvatori, Ph.D., post-doctoral research fellow in Chamberlain's lab, reported the same results in mice with a normal immune system.

"Using this new version of our viral vector, we have induced stable production of dystrophin for at least four months in muscle fibers of adult, dystrophic mice with normal immune systems," Salvatori said. "Removing a reporter gene called LacZ from the vector was the key to reducing the vector's strong immunogenic effect. Although we still see a small immune response, it peaks after 30 days and does not appear to adversely affect the ability of mouse muscle to take up the vector and produce dystrophin."

A new cell packaging line, also under development at the U-M, will make it possible to produce large amounts of the vector without contamination by other proteins or viruses that could trigger an immune response in humans, according to Salvatori.

The result is significant, because stability and immune response were two major problems that remained to be solved in mice before the U-M's dystrophin vector could be tested for safety and effectiveness in humans. Chamberlain and Jerry Mendell, M.D., of Ohio State University Medical Center, plan to begin testing the vector for safety in humans in the spring of 1999.

Dystrophin is a protein critical for normal maintenance of muscle tissue. Muscular dystrophy is caused by mutations in a large, complex gene which contains instructions telling muscle cells how to produce dystrophin. Because they lack the genetic code to produce dystrophin, children with muscular dystrophy gradually lose muscle tissue and die of heart or respiratory failure.

For eight years, Chamberlain and his U-M research team have been overcoming technical obstacles to an effective gene therapy treatment for muscular dystrophy. They have focused on using modified adenoviruses---the same type of virus that causes colds---as delivery vehicles, because they have a natural ability to enter muscle cells and deliver the dystrophin gene. Chamberlain is a member of the U-M Department of Human Genetics and also is affiliated with the Center for Gene Therapy in the U-M Health System.

Current collaborators in the U-M program to develop new gene therapy viral vectors for dystrophin include Ph.D. research fellows Catherine Barjot, Catherine Begy, Christiana DelloRusso, Dennis Hartigan-O'Connor, Ann Saulino and Michael Hauser, who is now at Duke University.

The work is funded by the National Institutes of Health, the Muscular Dystrophy Association and a private foundation established by a Birmingham, Mich., couple, Chip and Betsy Erwin, to support the U-M research program.


Story Source:

The above story is based on materials provided by University Of Michigan. Note: Materials may be edited for content and length.


Cite This Page:

University Of Michigan. "Viral Vector Delivers Dystrophin Gene To Mouse Muscle." ScienceDaily. ScienceDaily, 4 November 1998. <www.sciencedaily.com/releases/1998/10/981031181529.htm>.
University Of Michigan. (1998, November 4). Viral Vector Delivers Dystrophin Gene To Mouse Muscle. ScienceDaily. Retrieved September 23, 2014 from www.sciencedaily.com/releases/1998/10/981031181529.htm
University Of Michigan. "Viral Vector Delivers Dystrophin Gene To Mouse Muscle." ScienceDaily. www.sciencedaily.com/releases/1998/10/981031181529.htm (accessed September 23, 2014).

Share This



More Health & Medicine News

Tuesday, September 23, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Ebola Costs Keep Mounting

Ebola Costs Keep Mounting

Reuters - Business Video Online (Sep. 23, 2014) The WHO has warned up to 20,000 people could be infected with Ebola over the next few weeks. As Sonia Legg reports, the implications for the West African countries suffering from the disease are huge. Video provided by Reuters
Powered by NewsLook.com
Liberia Pleads for Help to Fight Ebola

Liberia Pleads for Help to Fight Ebola

AP (Sep. 22, 2014) Liberia's finance minister is urging the international community to quickly follow through on pledges of cash to battle Ebola. Bodies are piling up in the capital Monrovia as the nation awaits more help. (Sept. 22) Video provided by AP
Powered by NewsLook.com
Ebola Doctor Says Border Controls Critical

Ebola Doctor Says Border Controls Critical

AP (Sep. 22, 2014) A Florida doctor who helped fight the expanding Ebola outbreak in West Africa says the disease can be stopped, but only if nations quickly step up their response and make border control a priority. (Sept. 22) Video provided by AP
Powered by NewsLook.com
Global Ebola Aid Increasing But Critics Say It's Late

Global Ebola Aid Increasing But Critics Say It's Late

Newsy (Sep. 21, 2014) More than 100 tons of medical supplies were sent to West Africa on Saturday, but aid workers say the global response is still sluggish. Video provided by Newsy
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:

Breaking News:

Strange & Offbeat Stories


Health & Medicine

Mind & Brain

Living & Well

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile: iPhone Android Web
Follow: Facebook Twitter Google+
Subscribe: RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins