Featured Research

from universities, journals, and other organizations

Scientists Develop Protein Nanoarrays For Biological Detection

Date:
February 11, 2002
Source:
Northwestern University
Summary:
Scientists at Northwestern University have developed a new detection technology on the nanometer scale that could lead to the next generation of proteomic arrays and new methods for diagnosing infectious diseases.

EVANSTON, Ill. — Scientists at Northwestern University have developed a new detection technology on the nanometer scale that could lead to the next generation of proteomic arrays and new methods for diagnosing infectious diseases.

Once optimized, the new nanotechnology holds promise for biological detectors that can yield more information more accurately in a shorter period of time. Such devices ultimately could be used in the doctor’s office to rapidly screen for a wide range of pathogenic diseases or in the field to detect biological weapons such as anthrax and smallpox.

A report on the protein nanoarrays was published online Feb. 7 by the journal Science at the Science Express Web site (http://www.sciencexpress.org).

Genetic and proteomic screening with so-called gene-chips and proteomic arrays are allowing researchers to peer into the genetic code of individuals and develop leads for important therapeutic agents in the pharmaceutical industry. Current technology uses arrays of either proteins or DNA on the micrometer level as screening tools for analyzing DNA, protein-protein interactions and cell biology and for drug testing. Miniaturizing these arrays could dramatically improve their capabilities.

The researchers utilize a process invented at Northwestern called Dip-Pen Nanolithography to make arrays of proteins with features more than 1,000 times smaller than those used in conventional arrays. This leads to nanoarrays with more than 1 million times the density of current commercial microarrays.

Led by Chad A. Mirkin, director of Northwestern’s Institute for Nanotechnology, the research team combined expertise with Professor Milan Mrksich of the University of Chicago and his group and also showed that the novel arrays could be used to study important biological processes, such as cell adhesion. This involves discovering and then writing a pattern of proteins that attracts a particular molecule.

"Our technology opens up many new possibilities for detection and understanding the interactions of biomolecules with each other and synthetic agents," said Mirkin. "We have developed a simple way of recognizing complex materials. Once the pattern of protein dots that matches a particular biomolecule or structure is known, we can build a detector for that biomolecule. This means that instead of testing for anthrax DNA, which requires a lot of processing, we might be able to test for the anthrax spore itself."

"Creating patterns on a sub-micrometer level is important," added Mirkin, also George B. Rathmann Professor of Chemistry. "More detailed questions can be asked and answered when working on the nanometer scale. This is a fundamental advance in biorecognition."

Mirkin’s method of Dip-Pen Nanolithography allowed the researchers to use an atomic force microscope tip as a nano-pen to write out a tiny protein array on a gold surface. With an array of protein "dots" as small as 100 nanometers in diameter, the gold surface in between the dots was processed to prevent it from absorbing target proteins and disturbing the readings. (A nanometer is one-billionth of a meter.) When an array on a chip was exposed to protein targets in solution, the protein on the substrate (16-mercaptohexadecanoic acid or MHA) bound its complementary proteins (lysozyme and rabbit immunoglobin). The atomic force microscope then read the chip and recorded a match where a change in height was detected.

Other authors on the paper are Ki-Bum Lee (lead author) and So-Jung Park, both of Northwestern. The research was supported by the Air Force Office of Scientific Research, the Defense Advanced Research Projects Agency (DARPA), the National Science Foundation and the National Institutes of Health.


Story Source:

The above story is based on materials provided by Northwestern University. Note: Materials may be edited for content and length.


Cite This Page:

Northwestern University. "Scientists Develop Protein Nanoarrays For Biological Detection." ScienceDaily. ScienceDaily, 11 February 2002. <www.sciencedaily.com/releases/2002/02/020208075550.htm>.
Northwestern University. (2002, February 11). Scientists Develop Protein Nanoarrays For Biological Detection. ScienceDaily. Retrieved July 24, 2014 from www.sciencedaily.com/releases/2002/02/020208075550.htm
Northwestern University. "Scientists Develop Protein Nanoarrays For Biological Detection." ScienceDaily. www.sciencedaily.com/releases/2002/02/020208075550.htm (accessed July 24, 2014).

Share This




More Matter & Energy News

Thursday, July 24, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Robot Parking Valet Creates Stress-Free Travel

Robot Parking Valet Creates Stress-Free Travel

AP (July 23, 2014) 'Ray' the robotic parking valet at Dusseldorf Airport in Germany lets travelers to avoid the hassle of finding a parking spot before heading to the check-in desk. (July 23) Video provided by AP
Powered by NewsLook.com
Boeing Ups Outlook on 52% Profit Jump

Boeing Ups Outlook on 52% Profit Jump

Reuters - Business Video Online (July 23, 2014) Commercial aircraft deliveries rose seven percent at Boeing, prompting the aerospace company to boost full-year profit guidance- though quarterly revenues missed analyst estimates. Bobbi Rebell reports. Video provided by Reuters
Powered by NewsLook.com
Europe's Car Market on the Rebound?

Europe's Car Market on the Rebound?

Reuters - Business Video Online (July 23, 2014) Daimler kicks off a round of second-quarter earnings results from Europe's top carmakers with a healthy set of numbers - prompting hopes that stronger sales in Europe will counter weakness in emerging markets. Hayley Platt reports. Video provided by Reuters
Powered by NewsLook.com
9/11 Commission Members Warn of Terror "fatigue" Among American Public

9/11 Commission Members Warn of Terror "fatigue" Among American Public

Reuters - US Online Video (July 22, 2014) Ten years after releasing its initial report, members of the 9/11 Commission warn of the "waning sense of urgency" in combating terrorists attacks. Mana Rabiee reports. Video provided by Reuters
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:

Breaking News:
from the past week

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile: iPhone Android Web
Follow: Facebook Twitter Google+
Subscribe: RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins