Featured Research

from universities, journals, and other organizations

Virus That Can Infect Both Mice And Humans Is Driven To Extinction With Cancer Drug

Date:
September 4, 2002
Source:
Cedars-Sinai Medical Center
Summary:
For decades, scientists have tried to figure out how to stop viruses from spreading. Their efforts have led to the development of drugs that can help to slow the spread of some infections and diseases such as AIDS by preventing viruses from reproducing. But because viruses' genetic machinery has the ability to replicate and mutate so quickly, viruses rapidly become resistant to individual anti-viral drugs.

LOS ANGELES, CA (Embargoed until 5 p.m. EST on September 2, 2002) -- For decades, scientists have tried to figure out how to stop viruses from spreading. Their efforts have led to the development of drugs that can help to slow the spread of some infections and diseases such as AIDS by preventing viruses from reproducing. But because viruses' genetic machinery has the ability to replicate and mutate so quickly, viruses rapidly become resistant to individual anti-viral drugs. Thus, the effectiveness of these drugs is very limited.

Now, researchers currently at Cedars-Sinai Medical Center, and the Universidad Autonoma de Madrid, in Spain, report that treating a common virus with a mutation-causing cancer drug caused the virus to mutate so much that it was no longer able to reproduce and was driven to extinction. The findings, reported in the current issue of the Proceedings of the National Sciences (PNAS), and preceded by a Commentary by Nobel-Prize winning Professor Manfred Eigen from the Max Planck Institut fur biophysikalische Chemie, Goettingen, Germany, may ultimately lead to new ways to treat and eliminate viral infections.

"We found that a specific chemical mutagen caused the virus to mutate so much that the replication process was ultimately aborted," said Pedro Lowenstein, M.D., Ph.D., Director of the Board of Governor's Gene Therapeutics Research Institute at Cedars-Sinai Medical Center. "These findings, may lead, in the future, to ways to stop viruses from reproducing in humans in the same way."

"Although the possibility to exploit high mutation rates in viruses was already known, this and other work is providing the basis for more rapid progress in this field," said collaborator and co-author Esteban Domingo, Ph.D, Director of the Laboratory of Genetic Variability of RNA Viruses at the Center for Molecular Biology 'Severo Ochoa', Universidad Autonoma de Madrid, Spain.

Viruses are genetic entities that can cause a number of infections and diseases in humans such as the common cold, chickenpox, shingles, herpes, polio, influenza and AIDS. Infections occur when a virus comes into contact with a host cell and injects its genetic material, literally taking over the cell's function. Subsequently, the infected cell produces more viral protein and genetic material instead of its usual products.

But for viruses to reproduce and spread, they must make many copies of their genetic material. In RNA viruses, such as those discussed here, this process occurs when a viral enzyme reads the sequence of the viruses' genome within the host cell and transcribes it into a complementary RNA or DNA sequence. Yet, the process is error-prone, as mistakes occur during replication when the genome sequence is read and transcribed, resulting in mutations in the viral genome.

Capitalizing on the viruses' tendency to make errors during the replicating process, the investigators tested two types of mutation-inducing cancer drugs in cells infected with lymphocytic choriomeningitis virus (LCMV), a virus that can infect both mice and humans. To do this, the investigators infected hamster cells, grown in culture, with the virus and treated them with either 5-fluorouracil (FU) or 5-azacytidine (AZC). The viruses' ability to replicate and the amount of mutations that occurred in the viral genomes was then measured and compared at varying doses of either FU or AZC. The researchers found that both FU and AZC increased the number of mutations in all areas of the viruses' genetic machinery even at lower doses. However, only higher doses of FU were able to drive the virus to extinction, while treatment with AZC at any dose level was not effective to eliminate the virus.

"Given that FU eliminated this particular virus, while AZC did not suggests that different mutagens will be more effective than others in the treatment of viruses," commented Dr. Lowenstein. "This work also lays the groundwork for future studies to determine how mutagens actually slow down the replication process and, at the same time, may lead to new treatments that succeed where others have limited effectiveness."

Although viral extinction was likely due to an increase in the mutation rate caused by FU, the investigators were unable to track the viruses' mutations as they progressed closer to extinction.

"This was likely due to the fact that highly mutated genomes are either undetectable with current methods, or that the mutations obtained and detected were already sufficient to block further viral replication," said Dr. Lowenstein.

"That the introduction of more mutations could help combat viral disease seemed totally unrealistic a decade ago. Now, despite obvious difficulties and a lot of work to be done, it is a sound scientific proposition," commented Dr. Domingo.

Cedars-Sinai Medical Center is one of the largest nonprofit academic medical centers in the Western United States. For the fifth straight two-year period, Cedars-Sinai has been named Southern California's gold standard in health care in an independent survey. Cedars-Sinai is internationally renowned for its diagnostic and treatment capabilities and its broad spectrum of programs and services, as well as breakthrough in biomedical research and superlative medical education. Named one of the 100 "Most Wired" hospitals in health care in 2001, the Medical Center ranks among the top 10 non-university hospitals in the nation for its research activities.


Story Source:

The above story is based on materials provided by Cedars-Sinai Medical Center. Note: Materials may be edited for content and length.


Cite This Page:

Cedars-Sinai Medical Center. "Virus That Can Infect Both Mice And Humans Is Driven To Extinction With Cancer Drug." ScienceDaily. ScienceDaily, 4 September 2002. <www.sciencedaily.com/releases/2002/09/020904074147.htm>.
Cedars-Sinai Medical Center. (2002, September 4). Virus That Can Infect Both Mice And Humans Is Driven To Extinction With Cancer Drug. ScienceDaily. Retrieved September 23, 2014 from www.sciencedaily.com/releases/2002/09/020904074147.htm
Cedars-Sinai Medical Center. "Virus That Can Infect Both Mice And Humans Is Driven To Extinction With Cancer Drug." ScienceDaily. www.sciencedaily.com/releases/2002/09/020904074147.htm (accessed September 23, 2014).

Share This



More Health & Medicine News

Tuesday, September 23, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Liberia Pleads for Help to Fight Ebola

Liberia Pleads for Help to Fight Ebola

AP (Sep. 22, 2014) Liberia's finance minister is urging the international community to quickly follow through on pledges of cash to battle Ebola. Bodies are piling up in the capital Monrovia as the nation awaits more help. (Sept. 22) Video provided by AP
Powered by NewsLook.com
Ebola Doctor Says Border Controls Critical

Ebola Doctor Says Border Controls Critical

AP (Sep. 22, 2014) A Florida doctor who helped fight the expanding Ebola outbreak in West Africa says the disease can be stopped, but only if nations quickly step up their response and make border control a priority. (Sept. 22) Video provided by AP
Powered by NewsLook.com
Global Ebola Aid Increasing But Critics Say It's Late

Global Ebola Aid Increasing But Critics Say It's Late

Newsy (Sep. 21, 2014) More than 100 tons of medical supplies were sent to West Africa on Saturday, but aid workers say the global response is still sluggish. Video provided by Newsy
Powered by NewsLook.com
Sierra Leone in Lockdown to Control Ebola

Sierra Leone in Lockdown to Control Ebola

AP (Sep. 21, 2014) Sierra Leone residents remained in lockdown on Saturday as part of a massive effort to confine millions of people to their homes in a bid to stem the biggest Ebola outbreak in history. (Sept. 20) Video provided by AP
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:

Breaking News:

Strange & Offbeat Stories


Health & Medicine

Mind & Brain

Living & Well

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile: iPhone Android Web
Follow: Facebook Twitter Google+
Subscribe: RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins