Featured Research

from universities, journals, and other organizations

Dartmouth Engineers Closer To Mass-producing Therapeutic Proteins

Date:
April 18, 2003
Source:
Dartmouth College
Summary:
Dartmouth engineers are one step closer to mass-producing therapeutic proteins desperately needed by today's pharmaceutical industry. Reported in the Proceedings of the National Academy Of Sciences, the researchers have achieved a major milestone in their efforts to effectively produce human therapeutics using a yeast-based protein expression system.

Hanover, N.H. (April 17, 2003) -- Dartmouth engineers are one step closer to mass-producing therapeutic proteins desperately needed by today's pharmaceutical industry.

Reported in today's early online edition of the Proceedings of the National Academy Of Sciences, the researchers have achieved a major milestone in their efforts to effectively produce human therapeutics using a yeast-based protein expression system.

The research is the result of a collaborative effort between Dartmouth researchers and a bioengineering startup called GlycoFi, Inc. Founded by two Dartmouth engineering professors, GlycoFi is advancing technological solutions for the safe, fast, and cost-effective mass-production of fully-humanized proteins. Protein-based biological drugs must be manufactured by living cells, which are genetically engineered to produce (or express) proteins that mimic the structures synthesized by humans. Current production of these therapeutic proteins is being pushed beyond capacity by exponential growth in the biopharmaceutical industry. GlycoFi's business is to engineer fungal expression systems that produce therapeutic proteins with human-like structures at an industrial scale.

"Production capacity has led to a bottleneck within the biopharmaceutical pipeline," said Charles Hutchinson, co-founder and CEO of GlycoFi, as well as dean emeritus of Dartmouth's Thayer School of Engineering. "The result is that some approved therapeutic protein drugs cannot be produced in adequate amounts, and still others are not making it into commercialization due to the cost and inefficiencies of producing them. It is our hope that this push to producing homogeneous, human-like glycoproteins in yeast will eliminate the production capacity bottleneck, and allow for the production of better and safer drugs."

Fungal-based protein expression systems are safer than conventional mammalian cell culture systems, but have not been effective in replicating complex human glycoprotein structures--until now.

"Demonstrating for the first time the production of 'hybrid' glycosylation structures in yeast brings GlycoFi an important step closer to dramatically improving the capacity and cost of producing therapeutic proteins," said Tillman Gerngross, Dartmouth engineering professor, co-founder and chief scientific officer of GlycoFi, and one of the authors on the paper. "In fact, we have already gone beyond this work and expect to manufacture fully complex human glycoproteins in one of our fungal production systems before the year's end."

Dartmouth/GlycoFi scientists genetically engineered the yeast P. pastoris to perform a series of sequential reactions that mimic the early processing of proteins in humans. After eliminating non-human glycosylation from the yeast, several genes were inserted into the yeast in such a way that the yeast synthesized new human-like glycosylation structures.

"The glycosylation structures we are seeing in our yeast are of a purity and uniformity unprecedented in biopharmaceutical manufacturing," said Stefan Wildt, also a Dartmouth engineering professor, director of strain development at GlycoFi, and another author of the paper. "This will allow GlycoFi to harness the inherent advantages of fungal protein expression systems and thereby address the biopharmaceutical manufacturing industry's capacity issues."


Story Source:

The above story is based on materials provided by Dartmouth College. Note: Materials may be edited for content and length.


Cite This Page:

Dartmouth College. "Dartmouth Engineers Closer To Mass-producing Therapeutic Proteins." ScienceDaily. ScienceDaily, 18 April 2003. <www.sciencedaily.com/releases/2003/04/030418081153.htm>.
Dartmouth College. (2003, April 18). Dartmouth Engineers Closer To Mass-producing Therapeutic Proteins. ScienceDaily. Retrieved April 18, 2014 from www.sciencedaily.com/releases/2003/04/030418081153.htm
Dartmouth College. "Dartmouth Engineers Closer To Mass-producing Therapeutic Proteins." ScienceDaily. www.sciencedaily.com/releases/2003/04/030418081153.htm (accessed April 18, 2014).

Share This



More Health & Medicine News

Friday, April 18, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Scientists Create Stem Cells From Adult Skin Cells

Scientists Create Stem Cells From Adult Skin Cells

Newsy (Apr. 17, 2014) The breakthrough could mean a cure for some serious diseases and even the possibility of human cloning, but it's all still a way off. Video provided by Newsy
Powered by NewsLook.com
Obama: 8 Million Healthcare Signups

Obama: 8 Million Healthcare Signups

AP (Apr. 17, 2014) President Barack Obama gave a briefing Thursday announcing 8 million people have signed up under the Affordable Care Act. He blasted continued Republican efforts to repeal the law. (April 17) Video provided by AP
Powered by NewsLook.com
Is Apathy A Sign Of A Shrinking Brain?

Is Apathy A Sign Of A Shrinking Brain?

Newsy (Apr. 17, 2014) A recent study links apathetic feelings to a smaller brain. Researchers say the results indicate a need for apathy screening for at-risk seniors. Video provided by Newsy
Powered by NewsLook.com
Could Even Casual Marijuana Use Alter Your Brain?

Could Even Casual Marijuana Use Alter Your Brain?

Newsy (Apr. 16, 2014) A new study conducted by researchers at Northwestern and Harvard suggests even casual marijuana use can alter your brain. Video provided by Newsy
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:

Breaking News:
from the past week

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile: iPhone Android Web
Follow: Facebook Twitter Google+
Subscribe: RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins