Featured Research

from universities, journals, and other organizations

First-ever Images Of Developing Dengue Virus Obtained At Purdue

Date:
June 19, 2003
Source:
Purdue University
Summary:
High-quality images of a virus still forming in its cellular host shed light on how viruses reproduce, knowledge that could prove important to the development of antiviral drugs.

WEST LAFAYETTE, Ind. - High-quality images of a virus still forming in its cellular host shed light on how viruses reproduce, knowledge that could prove important to the development of antiviral drugs.

Related Articles


A team including Purdue University's Michael Rossmann and Richard Kuhn has solved the structure of the immature dengue virus, which is related to West Nile virus and yellow fever. Dengue is a mosquito-borne pathogen that kills more than 24,000 people in the world annually. The pair solved the structure of the mature dengue virus particle last year (see related story), and Rossmann said the new findings were a significant step toward unraveling the behavior of viruses.

"We're beginning to dissect the individual steps in a virus' life cycle," said Rossmann, who is Henley Distinguished Professor of Biological Sciences in Purdue's School of Science. "We hope to learn a great deal more about viral development so that approaches to preventing infection become conceivable."

The study, a collaboration among Rossmann, Kuhn and Tim Baker at Purdue and James Straus at the California Institute of Technology, appears in the June 2 issue of EMBO.

The research group used an advanced imaging technique, known as cryoelectron microscopy, to take 3-D pictures of the dengue particle - the term experts use to denote a single virus. While viruses are not considered to be "alive" by the standards we apply to plants and animals, the team's images have revealed that particles go through a complex developmental process.

"We have discovered that an astonishing structural change occurs between the immature and mature dengue shells," said Kuhn, also professor of biology. "We don't yet know how it all happens - but even though we have only seen two points along the viral assembly line so far, we can tell it's quite a dynamic metamorphosis."

Compared to the mature dengue particle, for example, the immature form is 15 percent greater in diameter.

"The immature particle is covered with 60 three-pronged protein spikes, called trimers, that jut from its surface," Kuhn said. "In contrast, the mature particle is a nearly smooth sphere, like a golf ball. Somewhere in the assembly process, these trimers flatten out, making the surface appear more even."

The proteins are important because each contains a short amino acid sequence called a fusion peptide that the virus needs to attach itself to a potential host. Without this fusion peptide, the virus cannot successfully invade a cell.

"If you compare a virus to a pirate ship, these peptides are the grappling hooks by which they attach themselves to their prey," Kuhn said. "A particle can only inject its genetic material into a cell after it has bonded with its surface. Fusion peptides allow the virus to prepare for boarding, so to speak."

The peptides need to be protected until the virus is ready to bond with a cell, so in the immature particle, each peptide is covered with a special cap that protects it until the time is right.

"We would like to know more about how a virus changes," Rossmann said. "Our imaging techniques are now giving us vastly greater perspective on how a particle becomes a successful invader. Now we want to know how it marshals its offenses and defenses."

It is in examining the changes a virus undergoes- for example, in the case of dengue, how it uncaps its fusion peptides to become an infectious agent - that the team hopes to find clues to stopping the developmental process in its tracks.

"Any knowledge of the steps in a virus' assembly process provides a potential target for an antiviral agent," Rossmann said. "If you are trying to assemble something, introducing a foreign body into the process could gum up the works."

But Kuhn said much more work needs to be done before such medicines will appear in your drugstore, as the full picture of viral assembly remains unclear.

"This is only one step in the viral maturation process," Kuhn said. "We still need other scenes from its cycle of existence - snapshots of it fusing with a cell, for example, and of it entering - to have complete understanding."

The team's next step will be to confirm its findings, which Kuhn considers critical. The metamorphosis the dengue particle undergoes is so radical, he said, that there is a possibility the immature form the team has seen is not actually a step in dengue's development. For the moment, however, the results are encouraging enough to pursue the research further.

"Knowledge of how a virus assembles itself can reveal its vulnerabilities," Kuhn said. "This is what our research techniques allow us to explore - and perhaps exploit."

This research was funded in part by the Allergy and Infectious Diseases Institute at the National Institutes of Health.

Rossmann and Kuhn are associated with Purdue's Markey Center for Structural Biology, which consists of laboratories that use a combination of cryoelectron microscopy, crystallography and molecular biology to elucidate the processes of viral entry, replication and pathogenesis.


Story Source:

The above story is based on materials provided by Purdue University. Note: Materials may be edited for content and length.


Cite This Page:

Purdue University. "First-ever Images Of Developing Dengue Virus Obtained At Purdue." ScienceDaily. ScienceDaily, 19 June 2003. <www.sciencedaily.com/releases/2003/06/030619075237.htm>.
Purdue University. (2003, June 19). First-ever Images Of Developing Dengue Virus Obtained At Purdue. ScienceDaily. Retrieved November 26, 2014 from www.sciencedaily.com/releases/2003/06/030619075237.htm
Purdue University. "First-ever Images Of Developing Dengue Virus Obtained At Purdue." ScienceDaily. www.sciencedaily.com/releases/2003/06/030619075237.htm (accessed November 26, 2014).

Share This


More From ScienceDaily



More Health & Medicine News

Wednesday, November 26, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

From Popcorn To Vending Snacks: FDA Ups Calorie Count Rules

From Popcorn To Vending Snacks: FDA Ups Calorie Count Rules

Newsy (Nov. 25, 2014) — The US FDA is announcing new calorie rules on Tuesday that will require everywhere from theaters to vending machines to include calorie counts. Video provided by Newsy
Powered by NewsLook.com
Daily Serving Of Yogurt Could Reduce Risk Of Type 2 Diabetes

Daily Serving Of Yogurt Could Reduce Risk Of Type 2 Diabetes

Newsy (Nov. 25, 2014) — Need another reason to eat yogurt every day? Researchers now say it could reduce a person's risk of developing type 2 diabetes. Video provided by Newsy
Powered by NewsLook.com
Madagascar Working to Contain Plague Outbreak

Madagascar Working to Contain Plague Outbreak

AFP (Nov. 24, 2014) — Madagascar said Monday it is trying to contain an outbreak of plague -- similar to the Black Death that swept Medieval Europe -- that has killed 40 people and is spreading to the capital Antananarivo. Duration: 00:42 Video provided by AFP
Powered by NewsLook.com
Are Female Bosses More Likely To Be Depressed?

Are Female Bosses More Likely To Be Depressed?

Newsy (Nov. 24, 2014) — A new study links greater authority with increased depressive symptoms among women in the workplace. Video provided by Newsy
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
 
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:  

Breaking News:

Strange & Offbeat Stories

 

Health & Medicine

Mind & Brain

Living & Well

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:  

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile iPhone Android Web
Follow Facebook Twitter Google+
Subscribe RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins