Featured Research

from universities, journals, and other organizations

Livermore & NIH Scientists Create Technique To Examine Behavior Of Proteins At Single Molecule Level

Date:
August 29, 2003
Source:
Lawrence Livermore National Laboratory
Summary:
A Lawrence Livermore National Laboratory physicist, in collaboration with an international team of researchers, has developed an experimental method that allows scientists to investigate the behavior of proteins under non-equilibrium conditions one molecule at a time, to better understand a fundamental biological process of protein folding that is important for many diseases.

LIVERMORE, Calif. -- A Lawrence Livermore National Laboratory physicist, in collaboration with an international team of researchers, has developed an experimental method that allows scientists to investigate the behavior of proteins under non-equilibrium conditions one molecule at a time, to better understand a fundamental biological process of protein folding that is important for many diseases.

Related Articles


The work, presented in the Aug. 29 edition of Science, marks the first time protein-folding kinetics has been monitored on the single-molecule level. Proteins are long chains of amino acids. Like shoelaces, they loop about each other or fold in a variety of ways, and only one way allows the protein to function properly. Just as a knotted shoelace can be a problem, a misfolded protein can do serious damage. Many diseases, such as Alzheimer's, cystic fibrosis, mad cow disease and many cancers result from misfolded protein.

Livermore's Lawrence postdoctoral fellow Olgica Bakajin worked with scientists from the NIDDK Laboratory of Chemical Physics at the National Institute of Health and the Physikalische Biochemie Universitδt Postadam in Germany to develop a microfluidic mixer for studies of protein folding. With this mixer, researchers were able to access information about the protein folding reaction that was never available from ensemble measurements or even from the newer single molecule equilibrium measurements.

"For the first time, in this experiment we were able to look at a protein on a single molecule level at defined times after the folding reaction was initiated," Bakajin said. "With this method we are able to see and isolate intermediate states that under equilibrium conditions only exist for a brief period of time.

"This is a fundamental science project. We would like to understand the sequence of events through which a protein goes from a random coil to its functional 'folded' form, and we've designed an instrument that can help us do this. Now the instrument can be used to study many different proteins so we can come up with some general rules as to how proteins fold."

Understanding of protein folding will contribute to better understanding of the diseases, which in turn will lead to better treatments. Founded in 1952, Lawrence Livermore National Laboratory is a national security laboratory, with a mission to ensure national security and apply science and technology to the important issues of our time. Lawrence Livermore National Laboratory is managed by the University of California for the U.S. Department of Energy's National Nuclear Security Administration.


Story Source:

The above story is based on materials provided by Lawrence Livermore National Laboratory. Note: Materials may be edited for content and length.


Cite This Page:

Share This


More From ScienceDaily



More Matter & Energy News

Monday, March 2, 2015

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Solar Plane Passes New Test Ahead of World Tour

Solar Plane Passes New Test Ahead of World Tour

AFP (Mar. 2, 2015) — A solar-powered plane made a third successful test flight in the United Arab Emirates on Monday ahead of a planned round-the-world tour to promote alternative energy. Duration: 01:05 Video provided by AFP
Powered by NewsLook.com
Electric Hydrofoiling Watercraft Delivers Eco-Friendly Thrills

Electric Hydrofoiling Watercraft Delivers Eco-Friendly Thrills

Reuters - Innovations Video Online (Mar. 2, 2015) — The Quadrofoil is a high-tech electric personal watercraft that its makers call a 'sports car for the water'. When it hits 10 km/h, the Slovenian-engineered Quadrofoil is lifted above the water onto four wing-like hydrofoils where it 'flies' above the surface with minimal water resistance. Matthew Stock reports. Video provided by Reuters
Powered by NewsLook.com
Smartphone Giants Unveil Latest Models at Technology Show

Smartphone Giants Unveil Latest Models at Technology Show

AFP (Mar. 2, 2015) — Mobile providers have been unveiling their upcoming models at the Mobile World Congress in Barcelona, showing off the latest in smartphone technology. Duration: 00:57 Video provided by AFP
Powered by NewsLook.com
The Tech Challenges Facing Automakers

The Tech Challenges Facing Automakers

Reuters - Business Video Online (Mar. 2, 2015) — This year's The International Motor Show is getting underway in Geneva. As Sonia Legg reports its taking place as Europe's beleaguered car industry finally starts showing signs of picking up. Video provided by Reuters
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
 
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:  

Breaking News:

Strange & Offbeat Stories

 

Space & Time

Matter & Energy

Computers & Math

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:  

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile iPhone Android Web
Follow Facebook Twitter Google+
Subscribe RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins