Featured Research

from universities, journals, and other organizations

ASU Researchers Demonstrate New Technique That Improves The Power Of Atomic Force Micrscopy

Date:
August 23, 2004
Source:
Arizona State University
Summary:
A team of researchers have developed a method that could vastly improve the ability of atomic force microscopes to "see" the chemical composition of a sample, follow variations of the sample, as well as map its topographic structure.

An artist's depiction shows an atomic force microscope probe (not to scale) 'fishing' for molecular sites recognized by an antibody tethered to the probe by a fine polymer thread. The new technique promises to vastly improve the capabilities of atomic force microscopy.
Credit: Image courtesy of Arizona State University

TEMPE, Ariz. – A team of researchers have developed a method that could vastly improve the ability of atomic force microscopes to "see" the chemical composition of a sample, follow variations of the sample, as well as map its topographic structure.

The advance could have significant implications for drug development by allowing scientists to monitor the effects of potential drugs on an ever-smaller scale, according to Stuart Lindsay, director of the Center for Single Molecule Biophysics at the Biodesign Institute at Arizona State University and a lead researcher on the project.

Lindsay, an ASU professor in the department of physics and astronomy said the new technique allows an atomic force microscope to "see," on a nanometer scale, the chemical composition of molecules.

"Atomic force microscopy has a resolution down to an atomic level, but until now it has been blind to identifying specific chemical compositions," Lindsay said.

The researchers -- Lindsay, Hongda Wang, Ralph Bash, Brian Ashcroft, and Dennis Lohr of Arizona State University; Cordula Stroh, Hermann Gruber and Peter Hinterdorfer of the Institute of Biophysics at the University of Lintz, Austria; and Jeremy Nelson of Molecular Imaging Corporation, Tempe, Ariz. -- present their findings in "Single Molecule Recognition Imaging Microscopy" in the current issue of the Proceedings of the National Academy of Sciences. The article is available on line at http://www4.nationalacademies.org/nas/nashome.nsf

"If you imagine that all proteins are shaped like Lego blocks, then conventional atomic force microscopy (AFM) is feeling the Lego blocks on the floor, but it can't tell the difference between one block and another," Lindsay explained. "What we have done, is allow the person sitting on the floor and feeling those blocks to open their eyes and see that there are red Lego blocks, green Lego blocks and yellow Lego blocks."

"This allows you to identify specific components in an image," he added. "It means you can now follow a complex process and see what's happening, at the molecular level, to one of the components. We are now giving AFM chemical sensitivity in much the way colored dyes gave optical microscopes optical sensitivity for much larger objects (~1 micron)."

Atomic force microscopes provide images on the nanometer scale by using a highly sensitive and tiny probe that is essentially pulled across a surface. By doing this, researchers can obtain topographical images down to a nanometer scale.

To use the AFM in its new mode, the researchers attached antibodies keyed to individual proteins to the tip of an AFM's probe. When an antibody reacts with the protein it is specifically targeted for, it creates a variance in the microscope's reading compared to a reading with a bare tip, thus showing the presence of a protein or other specific material in the region being scanned.

To help ensure that the antibody tipped probe is truly sensitive, a strand of polymer connects the antibody to the tip, providing a tether that allows the antibody to wiggle into position to better connect with the protein receptors. A magnetically excited cantilever makes the tip oscillate up and down to make the antibody disconnect and reconnect and keep the probe moving.

A key capability of this technique, Lindsay said, is that it allows researchers to see how components of a cell react on a molecular scale when they experience biological processes, such as their response to a specific chemical or compound. In this mode, it could provide researchers with a molecular "time-lapsed movie" of such reactions, which could lead to greater understanding of the chemical dynamics involved in how cells react to such stimuli.

Lindsay said the new AFM method could be significant for drug discovery.

"This development opens up the AFM as a research tool," Lindsay added. "The ability to identify the specific proteins on a membrane surface means you can take something very complex, like the surface of a human cell with all of the types of different receptors on it and ask questions about the local chemistry, like what is binding at those sites. That will provide the fundamental knowledge you need to develop new drugs."


Story Source:

The above story is based on materials provided by Arizona State University. Note: Materials may be edited for content and length.


Cite This Page:

Arizona State University. "ASU Researchers Demonstrate New Technique That Improves The Power Of Atomic Force Micrscopy." ScienceDaily. ScienceDaily, 23 August 2004. <www.sciencedaily.com/releases/2004/08/040817075728.htm>.
Arizona State University. (2004, August 23). ASU Researchers Demonstrate New Technique That Improves The Power Of Atomic Force Micrscopy. ScienceDaily. Retrieved October 23, 2014 from www.sciencedaily.com/releases/2004/08/040817075728.htm
Arizona State University. "ASU Researchers Demonstrate New Technique That Improves The Power Of Atomic Force Micrscopy." ScienceDaily. www.sciencedaily.com/releases/2004/08/040817075728.htm (accessed October 23, 2014).

Share This



More Matter & Energy News

Thursday, October 23, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

3D Printed Instruments Make Sweet Music in Sweden

3D Printed Instruments Make Sweet Music in Sweden

Reuters - Innovations Video Online (Oct. 23, 2014) — Students from Lund University's Malmo Academy of Music are believed to be the world's first band to all use 3D printed instruments. The guitar, bass guitar, keyboard and drums were built by Olaf Diegel, professor of product development, who says 3D printing allows musicians to design an instrument to their exact specifications. Matthew Stock reports. Video provided by Reuters
Powered by NewsLook.com
Chameleon Camouflage to Give Tanks Cloaking Capabilities

Chameleon Camouflage to Give Tanks Cloaking Capabilities

Reuters - Innovations Video Online (Oct. 22, 2014) — Inspired by the way a chameleon changes its colour to disguise itself; scientists in Poland want to replace traditional camouflage paint with thousands of electrochromic plates that will continuously change colour to blend with its surroundings. The first PL-01 concept tank prototype will be tested within a few years, with scientists predicting that a similar technology could even be woven into the fabric of a soldiers' clothing making them virtually invisible to the naked eye. Matthew Stock reports. Video provided by Reuters
Powered by NewsLook.com
Jet Sales Lift Boeing Profit 18 Pct.

Jet Sales Lift Boeing Profit 18 Pct.

Reuters - Business Video Online (Oct. 22, 2014) — Strong jet demand has pushed Boeing to raise its profit forecast for the third time, but analysts were disappointed by its small cash flow. Fred Katayama reports. Video provided by Reuters
Powered by NewsLook.com
Internet of Things Aims to Smarten Your Life

Internet of Things Aims to Smarten Your Life

AP (Oct. 22, 2014) — As more and more Bluetooth-enabled devices are reaching consumers, developers are busy connecting them together as part of the Internet of Things. (Oct. 22) Video provided by AP
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
 
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:  

Breaking News:

Strange & Offbeat Stories

 

Space & Time

Matter & Energy

Computers & Math

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:  

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile iPhone Android Web
Follow Facebook Twitter Google+
Subscribe RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins