Featured Research

from universities, journals, and other organizations

Stem Cells From Muscles Can Repair Cartilage

Date:
January 30, 2006
Source:
John Wiley & Sons, Inc.
Summary:
Researchers designed a study using muscle-derived stem cells genetically engineered with a therapeutic protein in an effort to repair articular cartilage defects in rats.

Damage to articular cartilage (cartilage covering the ends of bones where they meet in a joint) frequently occurs due to injury or illness, and can lead to degenerative disease. Treatments and experimental approaches to repair this articular cartilage have achieved limited results, but currently there is no method to fully restore this type of injured cartilage. Tissue engineering involving the delivery of therapeutic proteins to the injured site is a promising new approach to repairing articular cartilage. Previous studies have suggested that muscles contain stem cells that can develop in various ways, including into cells that lead to the formation of bone. In a study published in the February 2006 issue of Arthritis & Rheumatism (http://www.interscience.wiley.com/journal/arthritis), researchers designed a study using muscle-derived stem cells (MDSCs) genetically engineered with a therapeutic protein in an effort to repair articular cartilage defects in rats.

Led by Johnny Huard, PhD, director of the Growth and Development Laboratory at Children's Hospital of Pittsburgh and an associate professor in the departments of Orthopaedic Surgery and Molecular Genetics and Biochemistry and Bioengineering at the University of Pittsburgh School of Medicine, researchers induced damage to the knee joints in 36 12-week-old rats and divided them into three groups. Group 1 was treated with MDSCs embedded in fibrin glue. Group 2 was treated with MDSCs that had been cultured from 3-week-old rats and genetically engineered to express bone morphogenetic protein-4 (BMP-4). Group 3, the control group, was treated with fibrin glue.

The results showed well-integrated repaired tissue in the MDSC-B4 treatment group 8, 12 and 24 weeks after surgery, while the other two groups did not show as much improvement initially and by 24 weeks had deteriorated. "This finding indicates that continuous endogenous BMP-4 supplied by MDSCs genetically modified to express BMP-4 over an extended period of time can enhance articular cartilage healing," the authors state. They note that using fibrin glue enabled the cells to settle even in narrow clefts and has no adverse effects on cell viability.

The study also included culturing MDSC and MDSC-B4 in vitro in three different types of media and found that MDSC-B4 were able to differentiate into chondrogenic cells (cells that develop into cartilage) depending on the type of medium in which they grew. The addition of transforming grown factor beta 1 (TGFbeta1) to MDSC-B4 did not enhance chondrogenic differentiation.

The authors conclude that skeletal muscle is a promising source of cells that can differentiate into cartilage-producing cells. "The MDSCs used here served as good carriers of a therapeutic gene and enabled the delivery of appropriate amounts of BMP-4 protein to the injury site," they state, adding that the improved repair lasted for 24 weeks following transplantation. Further studies conducted by the authors (not yet published) have produced similar results. They conclude: "These findings suggest that BMP-4 gene therapy based on retrovirally transduced MDSCs is a potential strategy by which to improve articular cartilage healing."

In an accompanying editorial in the same issue, Mary B. Goldring of the New England Baptist Bone and Joint Institute and Harvard Medical School in Boston, MA, notes that although stem cells derived from bone marrow have been extensively investigated for the repair of cartilage defects, the current study is the first to investigate using enriched stem cells from muscle. The author states that the results are encouraging, especially the successful use of fibrin glue to fill in the defect, and that the study "provides proof-of-principle for performing MDSC implantation in cartilage of adult humans, since 12-week-old rats are considered to be young adults." Although recent studies have suggested that fatty tissue and tissue lining the space between joints (synovium) might also be sources of stem cells, obtaining these cells is more invasive than muscle biopsies. The author also notes that patients could potentially serve as donors to themselves, since the current study used MDSCs from juvenile rats and other studies have shown such transplants to be problematic in adults. She concludes: "Thus, further work is warranted to determine the chondroprogenitor potential of MDSCs in adult humans and their capacity to form cartilage in vivo."

###

Articles:
"Cartilage Repair Using Bone Morphogenetic Protein 4 and Muscle-Derived Stem Cells," Ryosuke Kuroda, Arvydas Usas, Seiji Kubo, Karin Corsi, Hairong Peng, Tim Rose, James Cummins, Freddie H. Fu, Johnny Huard, Arthritis & Rheumatism, February 2006; 54:2; pp. 433-442.

"Are Bone Morphogenetic Proteins Effective Inducers of Cartilage Repair? Ex Vivo Transduction of Muscle-Derived Stem Cells," Mary B. Goldring, Arthritis & Rheumatism, February 2006; 54:2; pp. 387-389.


Story Source:

The above story is based on materials provided by John Wiley & Sons, Inc.. Note: Materials may be edited for content and length.


Cite This Page:

John Wiley & Sons, Inc.. "Stem Cells From Muscles Can Repair Cartilage." ScienceDaily. ScienceDaily, 30 January 2006. <www.sciencedaily.com/releases/2006/01/060130030516.htm>.
John Wiley & Sons, Inc.. (2006, January 30). Stem Cells From Muscles Can Repair Cartilage. ScienceDaily. Retrieved October 1, 2014 from www.sciencedaily.com/releases/2006/01/060130030516.htm
John Wiley & Sons, Inc.. "Stem Cells From Muscles Can Repair Cartilage." ScienceDaily. www.sciencedaily.com/releases/2006/01/060130030516.htm (accessed October 1, 2014).

Share This



More Health & Medicine News

Wednesday, October 1, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Pregnancy Spacing Could Have Big Impact On Autism Risks

Pregnancy Spacing Could Have Big Impact On Autism Risks

Newsy (Oct. 1, 2014) A new study says children born less than one year and more than five years after a sibling can have an increased risk for autism. Video provided by Newsy
Powered by NewsLook.com
Robotic Hair Restoration

Robotic Hair Restoration

Ivanhoe (Oct. 1, 2014) A new robotic procedure is changing the way we transplant hair. The ARTAS robot leaves no linear scarring and provides more natural results. Video provided by Ivanhoe
Powered by NewsLook.com
Insertable Cardiac Monitor

Insertable Cardiac Monitor

Ivanhoe (Oct. 1, 2014) A heart monitor the size of a paperclip that can save your life. The “Reveal Linq” allows a doctor to monitor patients with A-Fib on a continuous basis for up to 3 years! Video provided by Ivanhoe
Powered by NewsLook.com
Attacking Superbugs

Attacking Superbugs

Ivanhoe (Oct. 1, 2014) Two weapons hospitals can use to attack superbugs. Scientists in Ireland created a new gel resistant to superbugs, and a robot that can disinfect a room in minutes. Video provided by Ivanhoe
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:

Breaking News:

Strange & Offbeat Stories


Health & Medicine

Mind & Brain

Living & Well

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile: iPhone Android Web
Follow: Facebook Twitter Google+
Subscribe: RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins