Featured Research

from universities, journals, and other organizations

Role For Proteomics In Identifying Hematologic Malignancies

Date:
January 15, 2007
Source:
Beth Israel Deaconess Medical Center
Summary:
Scientists have identified a set of biomarkers that could help clinicians identify a group of hematologic malignancies known as myelodysplastic syndromes (MDS), which affect approximately 300,000 individuals worldwide and often progress to acute myeloid leukemia.

Scientists have identified a set of biomarkers that could help clinicians identify a group of hematologic malignancies known as myelodysplastic syndromes (MDS), which affect approximately 300,000 individuals worldwide and often progress to acute myeloid leukemia.

Reported in the advance issue of the Proceedings of the National Academy of Sciences (which appears on-line the week of January 8) the findings point to a possible new diagnostic method for these malignancies, which occur when blood cells remain in an immature stage within the bone marrow and never sufficiently develop into the mature cells necessary for proper hematologic functioning. The study was led by researchers at Beth Israel Deaconess Medical Center (BIDMC) and Heinrich Heine University in Duesseldorf, Germany.

"Currently, a bone marrow biopsy is the only definitive means available to diagnose MDS," explains senior author Towia Libermann, PhD, Director of the Genomics Center at BIDMC and director of the Dana-Farber/Harvard Cancer Center Cancer Proteomics Core. "And since this group of malignancies primarily affects elderly patients, such a procedure is particularly arduous and sometimes impossible."

Therefore, first author Manuel Aivado, MD, PhD, a member of the Libermann laboratory and Lecturer in Medicine at Harvard Medical School (HMS), devised a clinical study to test whether serum proteomic profiling might be used to identify biomarkers for MDS.

The large-scale study of proteins -- including their expression, modification, composition, structure and function -- the field of proteomics is proving instrumental in the identification of molecular biomarkers, such as those that indicate a particular disease, according to Libermann, who is also Associate Professor of Medicine at HMS.

Aivado and Libermann used a combination of two technologies -- protein fractionation and mass spectrometry -- to create proteome profiles from the serum of 218 patients (representing clinical trial participants from both the MDS Study Group in Duesseldorf and from BIDMC). Through these profiles, the investigators were able to successfully distinguish between cases of MDS, healthy control subjects and cases of non-MDS-related cytopenias (blood cell disorders).

"Rather than uncovering a single biomarker, we were able to identify a protein signature [or spectrum], which reproducibly identified MDS patients among three separate and distinct patient cohorts," explains Libermann. "Since many patients with autoimmune disorders are treated with cytotoxic drugs such as azathioprine or methotrexate, they become cytopenic and may be suspected of having MDS. By using this new profile, the need for bone marrow biopsies might also be reduced among this patient population."

In the second part of the study, the authors identified two separate chemokines -- CXCL4 and CXCL7 -- the first such molecular biomarkers for advanced MDS.

"Proteomic profiling, using in-depth mass spectrometry, follows in the footsteps of genomics and represents a critical next step in understanding the pathophysiology of diseases," says Libermann. "This study demonstrated for the first time that proteomic profiling can be used for biomarker discovery and diagnostic evaluation of hematologic malignancies, an important step in refining the diagnosis and, eventually, the treatment of this devastating malignancy."

This study was funded, in part, by grants from the National Institutes of Health, as well as by Leukamic-Liga e.V., Duesseldorf, the Dr. Mildred Scheel Cancer Foundation and the Whitaker Foundation.

In addition to Libermann and Aivado, coauthors include BIDMC investigators Dimitrios Spentzos, MD, Franck Grall, PhD, Ulrich Steidl, MD, PhD, Hasan Otu, PhD, Akos Czibere, MD, Michelle Shayne, MD, and Wolf Prall, MD; Heinrich Heine University investigators Ulrich Germing, MD, Aristoteles Giagounidis, MD, Christof Iking-Konert, MD, Norbert Gattermann, MD, PhD, and Rainer Haas, MD; Harvard Partners Center for Genetics and Genomics investigators Gil Alterowitz, PhD and Marco Ramoni, PhD; Ciphergen Biosystems, Inc., investigators Xiao-Ying Meng and Eric Fung, MD, PhD; Giannoula Klement, MD, of Children's Hospital Boston; and Constantine Mitsiades, MD, of Dana-Farber/Harvard Cancer Center.


Story Source:

The above story is based on materials provided by Beth Israel Deaconess Medical Center. Note: Materials may be edited for content and length.


Cite This Page:

Beth Israel Deaconess Medical Center. "Role For Proteomics In Identifying Hematologic Malignancies." ScienceDaily. ScienceDaily, 15 January 2007. <www.sciencedaily.com/releases/2007/01/070111092753.htm>.
Beth Israel Deaconess Medical Center. (2007, January 15). Role For Proteomics In Identifying Hematologic Malignancies. ScienceDaily. Retrieved September 22, 2014 from www.sciencedaily.com/releases/2007/01/070111092753.htm
Beth Israel Deaconess Medical Center. "Role For Proteomics In Identifying Hematologic Malignancies." ScienceDaily. www.sciencedaily.com/releases/2007/01/070111092753.htm (accessed September 22, 2014).

Share This



More Health & Medicine News

Monday, September 22, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Sierra Leone in Lockdown to Control Ebola

Sierra Leone in Lockdown to Control Ebola

AP (Sep. 21, 2014) Sierra Leone residents remained in lockdown on Saturday as part of a massive effort to confine millions of people to their homes in a bid to stem the biggest Ebola outbreak in history. (Sept. 20) Video provided by AP
Powered by NewsLook.com
Sierra Leone's Nationwide Ebola Curfew Underway

Sierra Leone's Nationwide Ebola Curfew Underway

Newsy (Sep. 20, 2014) Sierra Leone is locked down as aid workers and volunteers look for new cases of Ebola. Video provided by Newsy
Powered by NewsLook.com
Changes Found In Brain After One Dose Of Antidepressants

Changes Found In Brain After One Dose Of Antidepressants

Newsy (Sep. 19, 2014) A study suggest antidepressants can kick in much sooner than previously thought. Video provided by Newsy
Powered by NewsLook.com
Could Grief Affect The Immune Systems Of Senior Citizens?

Could Grief Affect The Immune Systems Of Senior Citizens?

Newsy (Sep. 19, 2014) The study found elderly people are much more likely to become susceptible to infection than younger adults going though a similar situation. Video provided by Newsy
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:

Breaking News:
from the past week

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile: iPhone Android Web
Follow: Facebook Twitter Google+
Subscribe: RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins