Featured Research

from universities, journals, and other organizations

System For Expanding Stem Cells To Form Cartilage Tissue Under Development

Date:
April 20, 2007
Source:
Centre for Synthesis and Chemical Biology
Summary:
Knee osteoarthritis affects 30 million people worldwide, causing pain and joint stiffness and in severe cases restricted mobility. The limited ability of this tissue to repair itself means that surgical intervention is usually required and over 600,000 surgical procedures are performed each year in the US. Researchers have now developed an economical tissue engineering approach which could offer new possibilities for restoring damaged or lost knee cartilage tissue.

Monolayer growth of Chondroprogenitor Cells in T-flask (flat surface)
Credit: Image courtesy of Centre for Synthesis and Chemical Biology

Knee osteoarthritis affects 30 million people worldwide, causing pain and joint stiffness and in severe cases restricted mobility. The limited ability of this tissue to repair itself means that surgical intervention is usually required and over 600,000 surgical procedures are performed each year in the US.

Related Articles


Professor Mohamed Al-Rubeai, currently a UCD Professor of Biochemical Engineering and principal investigator with the Centre for Synthesis and Chemical Biology and UCD Conway Institute has developed an economical tissue engineering approach which could offer new possibilities for restoring damaged or lost knee cartilage tissue.

Transplantation therapy

One of the most successful therapies is cell transplantation which involves removing a patient's own mature cartilage cells known as chondrocytes and growing them in vitro using tissue culture techniques. Once the cells have multiplied the patient must then undergo a second surgical procedure for implanting them into the knee. The implanted chondrocytes will then help to produce healthy cartilage.

"There are a number of new transplantation products in clinical trials that all use chondrocytes," explains Professor Al-Rubeai. "However, these cells have limitations because when they divide they lose the potential to form cartilage and the overall treatment is expensive."

Tissue engineering using stem cells

While at the University of Birmingham, Professor Al-Rubeai with collaborators in the Smith & Nephew research centre decided to turn their attention to tissue culture techniques using adult stem cells, which retain their ability to form cartilage when grown in vitro and enable the generation of large cell banks.

"Routine tissue culturing methodologies cannot cope with the scale of cell production required to create world stem cell banks for engineering knee cartilage tissue," explains Professor Al-Rubeai.

His research group has optimised the tissue culture techniques so they can grow more stem cells in vitro which have the characteristics or morphology of in vivo stem cells.

"This is the first study to factor in economics. A key objective of our work is to develop a model for the biopharmaceutical industry by generating a cell bank using an affordable technique," continues Professor Al-Rubeai. "A 17-fold expansion factor was consistently achieved and large numbers of stem cells for tissue culture engineering were obtained."

Supporting stem cells

Once the stem cells are expanded the challenge is to engineer new cartilage tissue before implantation into the knee. To do this stem cells are supported on a bioactive scaffold which shapes the cells so they will provide a better match to the in vivo environment.

Engineers at the UCD School of Chemical and Bioprocess Engineering are now beginning to look at biodegradable gels to make a cartilage construct. These hydrogels can help form the new cartilage tissue and once implanted the gel will biodegrade.

"Presently we are using bovine stem cells but we would like to progress to using human stem cells," concludes Professor Al-Rubeai. "Our aim now is to collaborate with clinicians so we can move this work into the clinic."

Reference: Melero-Martin JM, Dowling MA, Smith M, Al-Rubeai M, Expansion of chondroprogenitor cells on macroporous microcarriers as an alternative to conventional monolayer systems, Biomaterials 2006, 27 (15): 2970-2979.


Story Source:

The above story is based on materials provided by Centre for Synthesis and Chemical Biology. Note: Materials may be edited for content and length.


Cite This Page:

Centre for Synthesis and Chemical Biology. "System For Expanding Stem Cells To Form Cartilage Tissue Under Development." ScienceDaily. ScienceDaily, 20 April 2007. <www.sciencedaily.com/releases/2007/04/070419101148.htm>.
Centre for Synthesis and Chemical Biology. (2007, April 20). System For Expanding Stem Cells To Form Cartilage Tissue Under Development. ScienceDaily. Retrieved December 17, 2014 from www.sciencedaily.com/releases/2007/04/070419101148.htm
Centre for Synthesis and Chemical Biology. "System For Expanding Stem Cells To Form Cartilage Tissue Under Development." ScienceDaily. www.sciencedaily.com/releases/2007/04/070419101148.htm (accessed December 17, 2014).

Share This


More From ScienceDaily



More Health & Medicine News

Wednesday, December 17, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

UN: Up to One Million Facing Hunger in Ebola-Hit Countries

UN: Up to One Million Facing Hunger in Ebola-Hit Countries

AFP (Dec. 17, 2014) Border closures, quarantines and crop losses in West African nations battling the Ebola virus could lead to as many as one million people going hungry, UN food agencies said on Wednesday. Duration: 00:52 Video provided by AFP
Powered by NewsLook.com
When You Lose Weight, This Is Where The Fat Goes

When You Lose Weight, This Is Where The Fat Goes

Newsy (Dec. 17, 2014) Can fat disappear into thin air? New research finds that during weight loss, over 80 percent of a person's fat molecules escape through the lungs. Video provided by Newsy
Powered by NewsLook.com
Why Your Boss Should Let You Sleep In

Why Your Boss Should Let You Sleep In

Newsy (Dec. 17, 2014) According to research out of the University of Pennsylvania, waking up for work is the biggest factor that causes Americans to lose sleep. Video provided by Newsy
Powered by NewsLook.com
Flu Outbreak Closing Schools in Ohio

Flu Outbreak Closing Schools in Ohio

AP (Dec. 17, 2014) A wave of flu illnesses has forced some Ohio schools to shut down over the past week. State officials confirmed one pediatric flu-related death, a 15-year-old girl in southern Ohio. (Dec. 17) Video provided by AP
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:

Breaking News:

Strange & Offbeat Stories


Health & Medicine

Mind & Brain

Living & Well

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile: iPhone Android Web
Follow: Facebook Twitter Google+
Subscribe: RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins