How To Switch Off Cancer Cell Genes: Epigenetic Silencing Involves Distinct Changes In DNA Structures

The findings will enable researchers to explore new therapies to switch the genes back on and may lead to novel treatments for human cancers, says study lead author Peter A. Jones, Ph.D., D.Sc., director of the USC/Norris Comprehensive Cancer Center and Distinguished Professor at the Keck School of Medicine of USC.

"The study shows for the first time exactly how genes get shut down in cancer cells," Jones says. "It identifies what the target looks like so that new therapies can be designed to turn them back on."

The study showed that silencing of transcription start sites in some cancer cells involves distinct changes in nucleosomal occupancy'or the density of nucleosomes'in the cell. Researchers found that three nucleosomes, almost completely absent from the start site in normal cells, are present in the methylated and silenced promoter, suggesting that epigenetic silencing may be accomplished by the stable placement of nucleosomes into previously vacant positions.

DNA cytosine methylation'the addition of a group of specific chemicals to a stretch of DNA that can lock or silence a gene'may ultimately lead to silencing by enabling the stable presence of nucleosomes at the start sites of cancer-related genes, the study suggests.

"We believe these findings will contribute to the development of cancer therapies," Jones says. "We were surprised to find how rigid the inactive structure is, and how rapidly it can be dissolved by drug treatment."

Findings are published in the Nov. 13 issue of the journal Cancer Cell. Joy C. Lin, Shinwu Jeong, Gangning Liang, Daiya Takai, Merhnaz Fatemi, Yvonne C. Tsai, Gerda Egger, Einav Nili Gal-Yam, Peter A. Jones, "Role of Nucleosomal Occupancy in the Epigenetic Silencing of the MLH1 CpG Island," Cancer Cell, Volume 12; Issue 5, November 2007. DOI: 10.1016/

The study was supported by a grant from the National Institutes of Health.