Featured Research

from universities, journals, and other organizations

Lab In A Drop: Pocket-sized PCR Rapid Test Including Sample Preparation

Date:
May 7, 2008
Source:
Wiley-Blackwell
Summary:
Jürgen Pipper and his team at the Institute of Bioengineering and Nanotechnology in Singapore have developed a rapid test for genetic diagnosis that combines the preparation of biological samples with a polymerase chain reaction on one chip.

Analysis and diagnosis in a chip format are coming of age, but their practical application has been limited because until now, the sample usually had to be prepared separately and on a nonminiaturized scale.
Credit: Copyright Wiley-VCH

Analysis and diagnosis in a chip format are coming of age, but their practical application has been limited because until now, the sample usually had to be prepared separately and on a nonminiaturized scale. Jürgen Pipper and his team at the Institute of Bioengineering and Nanotechnology in Singapore want to change this. They have now developed a rapid test for genetic diagnosis that combines the preparation of biological samples with a polymerase chain reaction (PCR) on one chip. As they report in the journal Angewandte Chemie, the “laboratory device” for all steps in this system is a single drop containing magnetic nanoparticles, which is moved across the chip by a magnetic field.

Related Articles


PCR allows gene sequences to be duplicated and identified—to identify a disease trigger, for example. In this process, the sample must cycle through a specific sequence of temperatures. Because of the slow heating and cooling processes, laboratory PCR usually takes several hours. The new chip PCR requires only minutes, including for the sample preparation.

In contrast to other chip-based methods, the actual sample, such as a drop of blood, can be placed directly on the PCR chip, where it is mixed with a drop that contains magnetic particles. These particles are equipped with antibodies on their surface, antibodies that bind specifically to the interesting cells in the blood. By moving a magnet underneath the chip, a droplet containing the bound magnetic particles is physically pulled out of the blood droplet and moved on to the next station—a droplet of washing liquid. The magnetic droplet is combined with the washing droplet and then pulled out again through movement of the magnet. Another droplet then delivers the enzymes and reagents necessary for cell disruption.

The last station is the PCR station. After combination with a reagent droplet, the magnetic droplet is moved around like a clockwork, passing again and again through four different zones set to the temperatures necessary for PCR. Each cycle lasts 8 seconds. A fluorescence detector over one of the zones monitors the progress of the PCR (real-time PCR) and indicates whether the desired gene sequence is present and in what amount.

With their new PCR chip, the researchers were able to isolate 30 cells implanted with the genetic information for a green-fluorescing protein from 25 µL of blood, concentrate them 100-fold, wash them, rupture them, and detect the gene for the green protein by real-time PCR—all within just 17 minutes!


Story Source:

The above story is based on materials provided by Wiley-Blackwell. Note: Materials may be edited for content and length.


Journal Reference:

  1. Juergen Pipper, Yi Zhang, Pavel Neuzil, Tseng-Ming Hsieh. Clockwork PCR Including Sample Preparation. Angewandte Chemie International Edition, 2008; 47 (21): 3900 DOI: 10.1002/anie.200705016

Cite This Page:

Wiley-Blackwell. "Lab In A Drop: Pocket-sized PCR Rapid Test Including Sample Preparation." ScienceDaily. ScienceDaily, 7 May 2008. <www.sciencedaily.com/releases/2008/05/080506120940.htm>.
Wiley-Blackwell. (2008, May 7). Lab In A Drop: Pocket-sized PCR Rapid Test Including Sample Preparation. ScienceDaily. Retrieved March 1, 2015 from www.sciencedaily.com/releases/2008/05/080506120940.htm
Wiley-Blackwell. "Lab In A Drop: Pocket-sized PCR Rapid Test Including Sample Preparation." ScienceDaily. www.sciencedaily.com/releases/2008/05/080506120940.htm (accessed March 1, 2015).

Share This


More From ScienceDaily



More Health & Medicine News

Sunday, March 1, 2015

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Could a $34 Smartphone Device Improve HIV Diagnosis in Africa?

Could a $34 Smartphone Device Improve HIV Diagnosis in Africa?

Reuters - Innovations Video Online (Feb. 27, 2015) — A dongle that plugs into a Smartphone mimics a lab-based blood test for HIV and syphilis and can detect the diseases in 15 minutes, say researchers. Tara Cleary reports. Video provided by Reuters
Powered by NewsLook.com
Doctor Says Head Transplants Possible Within Two Years

Doctor Says Head Transplants Possible Within Two Years

Buzz60 (Feb. 27, 2015) — An Italian doctor is saying he could stick someone&apos;s head onto someone else&apos;s body. Patrick Jones (@Patrick_E_Jones) reports. Video provided by Buzz60
Powered by NewsLook.com
How Your Dentist Could Help Screen You For Diabetes

How Your Dentist Could Help Screen You For Diabetes

Newsy (Feb. 27, 2015) — A new study from researchers at New York University suggests dentists could soon use blood samples taken from patients&apos; mouths to test for diabetes. Video provided by Newsy
Powered by NewsLook.com
The Best Tips to Makeover Your Health

The Best Tips to Makeover Your Health

Buzz60 (Feb. 27, 2015) — If you&apos;re looking to boost your health this season, there are a few quick and easy steps to prompt you for success. Krystin Goodwin (@Krystingoodwin) has the best tips to give your health a makeover this spring! Video provided by Buzz60
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
 
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:  

Breaking News:

Strange & Offbeat Stories

 

Health & Medicine

Mind & Brain

Living & Well

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:  

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile iPhone Android Web
Follow Facebook Twitter Google+
Subscribe RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins