Featured Research

from universities, journals, and other organizations

Cancer Stem Cells Generated By Cancer Outgrowth

Date:
April 5, 2009
Source:
Cell Press
Summary:
Scientists have discovered that growing mouse skin cells in spheres can lead to generation of cells with properties of cancer stem cells, even without genetic manipulation of stem cell genes. This unexpected finding provides a potential pathway for generation of cancer stem cells from differentiated cells and may even eventually lead to safer strategies for creation of induced pluripotent stem cells for use in regenerative therapies.

Scientists have discovered that growing mouse skin cells in spheres can lead to generation of cells with properties of cancer stem cells, even without genetic manipulation of stem cell genes. This unexpected finding, published in the April 3rd issue of the journal Cell Stem Cell, provides a potential pathway for generation of cancer stem cells from differentiated cells and may even eventually lead to safer strategies for creation of induced pluripotent stem cells for use in regenerative therapies.

"A hallmark of all solid tumors is the outgrowth of cancer cells into three-dimensional structures," explains senior study author, Dr. Douglas C. Dean, from the University of Louisville Health Sciences Center in Louisville, Kentucky. Dr. Dean and colleagues examined whether abnormal cell configurations might trigger reprogramming of differentiated cells into cells that resembled cancer stem cells.

The researchers observed that mutation of all of the retinoblastoma tumor suppressor gene (RB1) family members, known to be critical for regulating cell-contact inhibition and restricting growth of normal cells into three-dimensional tumor-like structures, led to an outgrowth of cells into spheres that triggered generation of cells similar to cancer stem cells. Surprisingly, the cancer stem cell-like cells expressed key genes expressed in embryonic stem cells and gave rise to a variety of differentiated cells.

Interestingly, cells with only one RB1 mutation remained contact inhibited, but when mechanically scraped off the dish and forced to form spheres, they also exhibited cancer stem-like characteristics. Even cells with intact RB1 genes could be forced to form spheres, suggesting that the reprogramming did not require the loss of RB1. The researchers went on to show that the cancer stem-like cells isolated from the spheres with disrupted RB1 genes formed tumors when injected into mice and differentiated into mature cells in advancing cancers.

These results using cultured cells lead the authors to hypothesize that cancer stem cells may be generated as a direct function of the outgrowth of cells in the animal. "To our knowledge, this is the first example that silenced endogenous embryonic stem cell genes can be spontaneously reactivated in differentiated cells," says Dr. Dean. "We propose that the loss of cell contact inhibition when the RB1 pathway is inhibited leads to outgrowth into sphere-like structures, and these conditions in the advancing cancer trigger reprogramming of differentiated cells to cells with properties of cancer stem cells."

The researchers include Yongqing Liu, Brian Clem, Ewa K. Zuba-Surma, Shahenda El-Naggar, Sucheta Telang, Alfred B. Jenson, Yali Wang, Hui Shao, Mariusz Z. Ratajczak, Jason Chesney, and Douglas C. Dean, of University of Louisville Health Sciences Center, Louisville, KY.


Story Source:

The above story is based on materials provided by Cell Press. Note: Materials may be edited for content and length.


Cite This Page:

Cell Press. "Cancer Stem Cells Generated By Cancer Outgrowth." ScienceDaily. ScienceDaily, 5 April 2009. <www.sciencedaily.com/releases/2009/04/090402124244.htm>.
Cell Press. (2009, April 5). Cancer Stem Cells Generated By Cancer Outgrowth. ScienceDaily. Retrieved July 24, 2014 from www.sciencedaily.com/releases/2009/04/090402124244.htm
Cell Press. "Cancer Stem Cells Generated By Cancer Outgrowth." ScienceDaily. www.sciencedaily.com/releases/2009/04/090402124244.htm (accessed July 24, 2014).

Share This




More Health & Medicine News

Thursday, July 24, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

New Painkiller Designed To Discourage Abuse: Will It Work?

New Painkiller Designed To Discourage Abuse: Will It Work?

Newsy (July 24, 2014) The FDA approved Targiniq ER on Wednesday, a painkiller designed to keep users from abusing it. Like any new medication, however, it has doubters. Video provided by Newsy
Powered by NewsLook.com
Doctor At Forefront Of Fighting Ebola Outbreak Gets Ebola

Doctor At Forefront Of Fighting Ebola Outbreak Gets Ebola

Newsy (July 24, 2014) Sheik Umar Khan has treated many of the people infected in the Ebola outbreak, and now he's become one of them. Video provided by Newsy
Powered by NewsLook.com
Condemned Man's US Execution Takes Nearly Two Hours

Condemned Man's US Execution Takes Nearly Two Hours

AFP (July 24, 2014) America's death penalty debate raged Thursday after it took nearly two hours for Arizona to execute a prisoner who lost a Supreme Court battle challenging the experimental lethal drug cocktail. Duration: 00:55 Video provided by AFP
Powered by NewsLook.com
China's Ageing Millions Look Forward to Bleak Future

China's Ageing Millions Look Forward to Bleak Future

AFP (July 24, 2014) China's elderly population is expanding so quickly that children struggle to look after them, pushing them to do something unexpected in Chinese society- move their parents into a nursing home. Duration: 02:07 Video provided by AFP
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:

Breaking News:
from the past week

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile: iPhone Android Web
Follow: Facebook Twitter Google+
Subscribe: RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins