Featured Research

from universities, journals, and other organizations

Central Nervous System Repair? Neuronal Survival And Axonal Regrowth Obtained In Vitro

Date:
July 24, 2009
Source:
INSERM (Institut national de la santé et de la recherche médicale)
Summary:
While repair of the central nervous system has long been considered impossible, researchers have just developed a strategy that could promote neuronal regeneration after injury.

While repair of the central nervous system has long been considered impossible, French researchers from Inserm, the CNRS and the UPMC have just developed a strategy that could promote neuronal regeneration after injury. The in vitro studies have just been published in the journal PLoS One.

Related Articles


Repair of the central nervous system and restoration of voluntary motor activity through axonal re-growth has long been considered impossible in mammals. Over the last decade, numerous attempts proved disappointing overall. The Inserm team led by Alain Privat has recently shown that an essential component interfering with regeneration was due to the activity of astrocytes, feeder cells that surround neurons.

Normally, the primary role of astrocytes is to supply the nutrients necessary for neuronal function. In the event of spinal injury or lesion, astrocytes synthesize two particular proteins (glial fibrillary acidic protein (GFAP) and vimentin), which isolate the damaged neuron to prevent interference with the operation of the central nervous system.

While the protection is initially useful, in the long run it induces formation of impermeable cicatricial tissue around the neuron, thus constituting impenetrable scarring hostile to axonal regeneration and hence to propagation of nervous impulses. In the event of severe injury, the scarring engenders motor paralysis.

On the basis of the initial findings, the researchers pursued a strategy aimed at developing a therapeutic instrument to block formation of cicatricial tissue. In order to do so, they used gene therapy based on use of interfering RNA. The short RNA sequences, which were made to measure, were inserted into the cytoplasm of cultured astrocytes using a viral therapeutic vector. Once in the cell, the RNA activates mechanisms which block the synthesis of the two proteins secreted by astrocytes and responsible for cicatrix formation. Using that technique, the researchers succeeded in controlling the reaction of astrocytes and when the latter were cultured with neurons, they promoted neuronal survival and triggered axonal growth.

The promising results are now to be validated by in vivo studies. The next stage of the work, currently ongoing, applies the same method to the mouse. The approach may be used in the future in patients having undergone spinal injury.


Story Source:

The above story is based on materials provided by INSERM (Institut national de la santé et de la recherche médicale). Note: Materials may be edited for content and length.


Journal Reference:

  1. Desclaux Mathieu, Teigell Marisa, Amar Lahouari, Vogel Roland, Gimenez y Ribotta Minerva, Privat Alain and Mallet Jacques. A novel and efficient gene transfer strategy reduces glial scarring and improves neuronal survival and axonal growth in vitro. PLoS ONE, (in press)

Cite This Page:

INSERM (Institut national de la santé et de la recherche médicale). "Central Nervous System Repair? Neuronal Survival And Axonal Regrowth Obtained In Vitro." ScienceDaily. ScienceDaily, 24 July 2009. <www.sciencedaily.com/releases/2009/07/090724113546.htm>.
INSERM (Institut national de la santé et de la recherche médicale). (2009, July 24). Central Nervous System Repair? Neuronal Survival And Axonal Regrowth Obtained In Vitro. ScienceDaily. Retrieved November 22, 2014 from www.sciencedaily.com/releases/2009/07/090724113546.htm
INSERM (Institut national de la santé et de la recherche médicale). "Central Nervous System Repair? Neuronal Survival And Axonal Regrowth Obtained In Vitro." ScienceDaily. www.sciencedaily.com/releases/2009/07/090724113546.htm (accessed November 22, 2014).

Share This


More From ScienceDaily



More Plants & Animals News

Saturday, November 22, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Anglerfish Rarely Seen In Its Habitat Will Haunt You

Anglerfish Rarely Seen In Its Habitat Will Haunt You

Newsy (Nov. 22, 2014) — For the first time Monterey Bay Aquarium recorded a video of the elusive, creepy and rarely seen anglerfish. Video provided by Newsy
Powered by NewsLook.com
Birds Around the World Take Flight

Birds Around the World Take Flight

Reuters - Light News Video Online (Nov. 22, 2014) — An imperial eagle equipped with a camera spreads its wings over London. It's just one of the many birds making headlines in this week's "animal roundup". Jillian Kitchener reports. Video provided by Reuters
Powered by NewsLook.com
Could Your Genes Be The Reason You're Single?

Could Your Genes Be The Reason You're Single?

Newsy (Nov. 21, 2014) — Researchers in Beijing discovered a gene called 5-HTA1, and carriers are reportedly 20 percent more likely to be single. Video provided by Newsy
Powered by NewsLook.com
Raw: Baby Okapi Born at Houston Zoo

Raw: Baby Okapi Born at Houston Zoo

AP (Nov. 20, 2014) — The Houston Zoo released video of a male baby okapi. Okapis, also known as the "forest giraffe", are native to the Democratic Republic of the Congo in Central Africa. Video is mute from source. (Nov. 20) Video provided by AP
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
 
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:  

Breaking News:

Strange & Offbeat Stories

 

Plants & Animals

Earth & Climate

Fossils & Ruins

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:  

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile iPhone Android Web
Follow Facebook Twitter Google+
Subscribe RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins