Featured Research

from universities, journals, and other organizations

Improvement Of Liver Stem Cell Engraftment By Protein Delivery

Date:
September 14, 2009
Source:
Society for Experimental Biology and Medicine
Summary:
Researchers have engineered a chimeric protein that increases cell survival, migration and proliferation to improve stem cell engraftment. The results show that TAT-Tpr-Met, a cell permeable form of the hepatocyte growth factor receptor can increase the number of hepatic stem cells integrated into the liver of the mouse.

Researchers at INSERM (France) have engineered a chimeric protein that increases cell survival, migration and proliferation to improve stem cell engraftment.

The results, which appear in the September 2009 issue of Experimental Biology and Medicine, show that TAT-Tpr-Met, a cell permeable form of the hepatocyte growth factor receptor can increase the number of hepatic stem cells integrated into the liver of the mouse.

TAT-Tpr-Met is the result of the fusion of Tpr-Met, an autoactived tyrosine kinase, to the protein transduction domain from HIV-TAT that gives the potential of the whole protein to enter into cells. TAT-Tpr-Met enters into cells in just one hour, where it then remains stable and recapitulates several effects seen with the hepatocyte growth factor (HGF). As the activating signal induced by TAT-Tpr-Met comes from inside the cells, it is independent of the extracellular environment, and continues even if cells are placed in vivo. This property was used to transplant cells with an advantage that allows cells pretreated with TAT-Tpr-Met to engraft twice more than untreated cells.

The research team, Guillaume Kellermann, a graduate student in Biotechnology (University Paris VII), along with Lyes Boudechice, a veterinarian surgeon, Dr. Anne Weber and Dr. Michelle Hadchouel performed the studies in six week old mice. Dr. Kellermann noted that "previous work had already shown that cells engraft better with HGF, but contrary to other strategies, our method is virus and DNA free, so it may be safe for humans. However, before, considering clinical applications further studies need to be performed to check for the long term effects."

In summary, stem cells have an enormous potential in cell therapy, however their ability to engraft in solid tissues remains low. Here, a chimeric protein was engineered that switches them to a state more favorable to engraftment by promoting their survival, migration and proliferation. After a few days the protein is completely degraded inside the cells, therefore our method is safer than other strategies using modified virus and may be compatible with clinical applications.

Dr. Steven R. Goodman, Editor-in-Chief of Experimental Biology and Medicine, said: "The authors have reported a very clever approach, utilizing the fusion protein TAT-Tpr-Met, to increase hepatic stem cell engraftment into the liver. The fact that the approach does not require virus or DNA makes it a reasonable strategy for future clinical applications."


Story Source:

The above story is based on materials provided by Society for Experimental Biology and Medicine. Note: Materials may be edited for content and length.


Cite This Page:

Society for Experimental Biology and Medicine. "Improvement Of Liver Stem Cell Engraftment By Protein Delivery." ScienceDaily. ScienceDaily, 14 September 2009. <www.sciencedaily.com/releases/2009/08/090824182445.htm>.
Society for Experimental Biology and Medicine. (2009, September 14). Improvement Of Liver Stem Cell Engraftment By Protein Delivery. ScienceDaily. Retrieved August 23, 2014 from www.sciencedaily.com/releases/2009/08/090824182445.htm
Society for Experimental Biology and Medicine. "Improvement Of Liver Stem Cell Engraftment By Protein Delivery." ScienceDaily. www.sciencedaily.com/releases/2009/08/090824182445.htm (accessed August 23, 2014).

Share This




More Health & Medicine News

Saturday, August 23, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Drug Used To Treat 'Ebola's Cousin' Shows Promise

Drug Used To Treat 'Ebola's Cousin' Shows Promise

Newsy (Aug. 21, 2014) An experimental drug used to treat Marburg virus in rhesus monkeys could give new insight into a similar treatment for Ebola. Video provided by Newsy
Powered by NewsLook.com
Two US Ebola Patients Leave Hospital Free of the Disease

Two US Ebola Patients Leave Hospital Free of the Disease

AFP (Aug. 21, 2014) Two American missionaries who were sickened with Ebola while working in Liberia and were treated with an experimental drug are doing better and have left the hospital, doctors say on August 21, 2014. Duration: 01:05 Video provided by AFP
Powered by NewsLook.com
Cadavers, a Teen, and a Medical School Dream

Cadavers, a Teen, and a Medical School Dream

AP (Aug. 21, 2014) Contains graphic content. He's only 17. But Johntrell Bowles has wanted to be a doctor from a young age, despite the odds against him. He was recently the youngest participant in a cadaver program at the Indiana University NW medical school. (Aug. 21) Video provided by AP
Powered by NewsLook.com
American Ebola Patients Released: What Cured Them?

American Ebola Patients Released: What Cured Them?

Newsy (Aug. 21, 2014) It's unclear whether the American Ebola patients' recoveries can be attributed to an experimental drug or early detection and good medical care. Video provided by Newsy
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:

Breaking News:
from the past week

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile: iPhone Android Web
Follow: Facebook Twitter Google+
Subscribe: RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins