Featured Research

from universities, journals, and other organizations

Complete chemokine profile of a cell

Date:
February 9, 2010
Source:
Journal of Clinical Investigation
Summary:
Chemokines are a large group of proteins whose predominant function is to direct cell migration. They regulate many physiological and pathophysiological processes, in particular in the immune system. Researchers have now developed a simple method to efficiently identify all the chemokines produced by a single cell type, something that has not been possible before.

Chemokines are a large group of proteins whose predominant function is to direct cell migration. They regulate many physiological and pathophysiological processes, in particular in the immune system.

Dirk Homann and colleagues, at University of Colorado Denver, Aurora, have now developed a simple method to efficiently identify all the chemokines produced by a single cell type, something that has not been possible before.

The method developed by Homann and colleagues is a flow cytometry-based assay that uses commercially available chemokine-specific antibodies to efficiently detect within cells 37 of 39 mouse chemokines. Using this technique, the author were able to delineate the complete chemokine profiles of two types of immune cell (NK cells and B cells) in response to major polyclonal stimuli and to assess the chemokine response of immune cells known as DCs to bacterial infection.

Importantly, the authors were able to adapt the method to analyze chemokine expression in human cells, leading them to suggest that this method will help researchers understand the roles of chemokines in health and disease.

The research appears in the Journal of Clinical Investigation.


Story Source:

The above story is based on materials provided by Journal of Clinical Investigation. Note: Materials may be edited for content and length.


Journal Reference:

  1. Jens Eberlein, Tom T. Nguyen, Francisco Victorino, Lucy Golden-Mason, Hugo R. Rosen, and Dirk Homann. Comprehensive assessment of chemokine expression profiles by flow cytometry. Journal of Clinical Investigation, 2010; DOI: 10.1172/JCI40645

Cite This Page:

Journal of Clinical Investigation. "Complete chemokine profile of a cell." ScienceDaily. ScienceDaily, 9 February 2010. <www.sciencedaily.com/releases/2010/02/100208215508.htm>.
Journal of Clinical Investigation. (2010, February 9). Complete chemokine profile of a cell. ScienceDaily. Retrieved October 20, 2014 from www.sciencedaily.com/releases/2010/02/100208215508.htm
Journal of Clinical Investigation. "Complete chemokine profile of a cell." ScienceDaily. www.sciencedaily.com/releases/2010/02/100208215508.htm (accessed October 20, 2014).

Share This



More Health & Medicine News

Monday, October 20, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Microneedle Patch Promises Painless Pricks

Microneedle Patch Promises Painless Pricks

Reuters - Innovations Video Online (Oct. 18, 2014) Researchers at The National University of Singapore have invented a new microneedle patch that could offer a faster and less painful delivery of drugs such as insulin and painkillers. Video provided by Reuters
Powered by NewsLook.com
Raw: Nurse Nina Pham Arrives in Maryland

Raw: Nurse Nina Pham Arrives in Maryland

AP (Oct. 17, 2014) The first nurse to be diagnosed with Ebola at a Dallas hospital walked down the stairs of an executive jet into an ambulance at an airport in Frederick, Maryland, on Thursday. Pham will be treated at the National Institutes of Health. (Oct. 16) Video provided by AP
Powered by NewsLook.com
Raw: Cruise Ship Returns to US Over Ebola Fears

Raw: Cruise Ship Returns to US Over Ebola Fears

AP (Oct. 17, 2014) A Caribbean cruise ship carrying a Dallas health care worker who is being monitored for signs of the Ebola virus is heading back to Texas, US, after being refused permission to dock in Cozumel, Mexico. (Oct. 17) Video provided by AP
Powered by NewsLook.com
Spanish Govt: Four Suspected Ebola Cases in Spain Test Negative

Spanish Govt: Four Suspected Ebola Cases in Spain Test Negative

AFP (Oct. 17, 2014) All four suspected Ebola cases admitted to hospitals in Spain on Thursday have tested negative for the deadly virus in a first round of tests, the government said Friday. Duration: 00:55 Video provided by AFP
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:

Breaking News:

Strange & Offbeat Stories


Health & Medicine

Mind & Brain

Living & Well

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile: iPhone Android Web
Follow: Facebook Twitter Google+
Subscribe: RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins