Featured Research

from universities, journals, and other organizations

Structure of inner-ear protein is key to both hearing and inherited deafness

Date:
April 17, 2010
Source:
Harvard Medical School
Summary:
Using a combination of crystallization and physics-based simulations, researchers defined the structure of a protein, cadherin-23, that helps mediate our perception of sound. Their findings show the protein to be a rigid structure whose strength results from calcium ions binding within it. However, mutations that interfere with calcium-ion binding undermine the protein's firm structure and make it less resilient. This provides a possible explanation for certain forms of inherited deafness.

Still image from a simulation that "stretches" the two end-most segments of the cadherin-23 protein, much as loud noise might do to its real-life counterpart in the inner ear. Such extreme stress can cause cadherin-23 to break in billionths of a second.
Credit: Marcos Sotomayor

Rising from the top surface of each of the specialized receptor cells in our inner ears is a bundle of sensory cilia that responds to the movement of sound. As sensitive as they are fragile, these cilia can move to wisps of sound no larger than a molecule -- but can shear at sounds that are larger than life.

Cilia function mechanically, fluttering back and forth in response to sound vibrations. The tug of the cilia creates tension in fine "tip links" that tether each cilium to its neighbor, causing them to pull open channels that allow ions to flow into the receptor cells. This ion movement into the receptor cells, called hair cells for their microscopic bundles of cilia, generates the electrical signals that go to the brain to be translated into the sound of, say, footsteps, or a sonata, or a helicopter landing.

The cilia-tip link apparatus in the hair cell is a clever arrangement of spring-like and string-like protein molecules, yet their atomic structures and mechanical properties are not well known.

Now new work from the laboratories of David Corey, professor of neurobiology at Harvard Medical School and Howard Hughes Medical Institute investigator, and Rachelle Gaudet, associate professor of molecular and cellular biology at Harvard University's Faculty of Arts and Sciences, has discovered how the structure of a vital region of cadherin-23, one of two proteins that join to form each tip link, relates to the hearing process. Researchers found cadherin-23 to be stronger and stiffer than previously thought, a characteristic produced by calcium ions that bind within folds of the protein and tie the folds together. Furthermore, they found that many mutations to cadherin-23 cause inherited deafness by weakening the ability of calcium to hold the protein together.

"Our findings help explain a whole class of inherited deafness," says Corey. "Many, many different mutations that cause inherited deafness are mutations specifically to the amino acids that bind the calcium. And these findings show how critical calcium ions, and the amino acid residues that bind them, are to the structure of this protein."

The research will appeared in the April 15 issue of Neuron.

To get an atomic-level view of how cadherin-23 behaves under normal -- and abnormal -- conditions, the scientists used a combination of protein crystallization and virtual-reality-like simulation that showed how the cadherin could break in billionths of a second.

Through crystallization, the researchers found that, like other members of the cadherin family, this protein contains three critical calcium ions between each of its 27 bead-like segments. The simulations showed that the ions formed a strong link between adjacent beads, sufficient to hold them together. In addition, a novel calcium-ion binding site was discovered at the very end of cadherin-23.

According to Corey, these binding sites "staple" the various subunits of cadherin-23 together and may also create the bond made with the second protein of the tip link.

To quantify exactly how strong these calcium-ion bonds were, the scientists loaded the protein's structure into a supercomputer, rendered its virtual likeness, then "stretched" the structure. They found that overall the protein acted more like a wire cable than a spring, as previously thought. This made it capable of withstanding forces ten-fold stronger than any that might be produced by normal sounds. When the scientists tested the structure against very great forces, however, it unraveled.

The researchers then elected to test how mutations similar to those associated with inherited deafness might alter cadherin-23's structure and affinity for calcium ions.

"We had the three-dimensional structure of the wild type protein," says Marcos Sotomayor, an HMS research fellow in neurobiology and co-lead author on the study, "and were able to obtain the structure of the mutated protein. With simulations, we could test both structures' elasticity and reactions to force."

The researchers found that some mutations reduced the ability of the protein to bind calcium ions. Simulations showed this change ultimately weakened the protein. In addition, simulations revealed that other deafness-causing mutations exhausted the ability of calcium to hold cadherin-23 together.

These findings, the researchers believe, offer insight that may one day better inform efforts to develop therapeutic interventions.

This research was funded by the National Institutes of Health, National Science Foundation, U.S. Department of Energy, Klingenstein Fellowship Award


Story Source:

The above story is based on materials provided by Harvard Medical School. Note: Materials may be edited for content and length.


Journal Reference:

  1. Marcos M. Sotomayor, Wilhelm Weihofen, Rachelle Gaudet, DavidP. Corey. Structural Determinants of Cadherin-23 Function in Hearing and Deafness. Biophysical Journal, 2010; 98 (3): 509a DOI: 10.1016/j.bpj.2009.12.2770

Cite This Page:

Harvard Medical School. "Structure of inner-ear protein is key to both hearing and inherited deafness." ScienceDaily. ScienceDaily, 17 April 2010. <www.sciencedaily.com/releases/2010/04/100416185146.htm>.
Harvard Medical School. (2010, April 17). Structure of inner-ear protein is key to both hearing and inherited deafness. ScienceDaily. Retrieved September 17, 2014 from www.sciencedaily.com/releases/2010/04/100416185146.htm
Harvard Medical School. "Structure of inner-ear protein is key to both hearing and inherited deafness." ScienceDaily. www.sciencedaily.com/releases/2010/04/100416185146.htm (accessed September 17, 2014).

Share This



More Health & Medicine News

Wednesday, September 17, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

President To Send 3,000 Military Personnel To Fight Ebola

President To Send 3,000 Military Personnel To Fight Ebola

Newsy (Sep. 16, 2014) President Obama is expected to send 3,000 troops to West Africa as part of the effort to contain Ebola's spread. Video provided by Newsy
Powered by NewsLook.com
Man Floats for 31 Hours in Gulf Waters

Man Floats for 31 Hours in Gulf Waters

AP (Sep. 16, 2014) A Texas man is lucky to be alive after he and three others floated for more than a day in the Gulf of Mexico when their boat sank during a fishing trip. (Sept. 16) Video provided by AP
Powered by NewsLook.com
Ivorians Abandon Monkey Pets in Fear Over Ebola Virus

Ivorians Abandon Monkey Pets in Fear Over Ebola Virus

AFP (Sep. 16, 2014) Since the arrival of Ebola in Ivory Coast, Ivorians have been abandoning their pets, particularly monkeys, in the fear that they may transmit the virus. Duration: 00:47 Video provided by AFP
Powered by NewsLook.com
Study Links Male-Pattern Baldness To Prostate Cancer

Study Links Male-Pattern Baldness To Prostate Cancer

Newsy (Sep. 16, 2014) New findings suggest men with a certain type of baldness at age 45 are 39 percent more likely to develop aggressive prostate cancer. Video provided by Newsy
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:

Breaking News:
from the past week

In Other News

... from NewsDaily.com

Science News

Health News

    Environment News

      Technology News



      Save/Print:
      Share:

      Free Subscriptions


      Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

      Get Social & Mobile


      Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

      Have Feedback?


      Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
      Mobile: iPhone Android Web
      Follow: Facebook Twitter Google+
      Subscribe: RSS Feeds Email Newsletters
      Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins