Featured Research

from universities, journals, and other organizations

How to rapidly assess children's tooth decay risk

Date:
July 2, 2010
Source:
Oregon Health & Science University
Summary:
Researchers have determined that ATP-driven (adenosine triphosphate-driven) bioluminescence -- a way of measuring visible light generated from ATP contained in bacteria -- is an innovative tool for rapidly assessing in children at the chair-side the number of oral bacteria and amount of plaque that can ultimately lead to tooth decay.

Researchers at the Oregon Health & Science University School of Dentistry have determined that ATP-driven (adenosine triphosphate-driven) bioluminescence -- a way of measuring visible light generated from ATP contained in bacteria -- is an innovative tool for rapidly assessing in children at the chair-side the number of oral bacteria and amount of plaque that can ultimately lead to tooth decay.

The study is published online in the May-June 2010 issue of Pediatric Dentistry.

Caries (microbial disease) prevention is one of the most important aspects of modern dental practice. Untreated, large numbers of cariogenic bacteria adhere to teeth and break down the protective enamel covering, resulting in lesions and cavities. There is a critical need in dentistry to develop better quantitative assessment methods for oral hygiene and to determine patient risk for dental caries, because disease as well as restorative treatment results in the irreversible loss of tooth structure. Previous caries risk assessments have focused on social, behavioral, microbiologic, environmental and clinical variables.

The goal of the OHSU study was to examine the use of microbiological testing, specifically ATP-driven bioluminescence, for quantifying oral bacteria, including plaque streptococci, and assessment of oral hygiene and caries risk. Thirty-three randomly selected OHSU pediatric patients, ages 7 to 12, were examined, and plaque specimens, in addition to saliva, were collected from one tooth in each of the four quadrants of the mouth. The oral specimens were then assessed to count total bacteria and streptococci and subjected to ATP-driven bioluminescence.

The OHSU team found statistical correlations, linking ATP to the numbers of total bacteria and oral streptococci. Their data indicated that ATP measurements have a strong statistical association with bacterial numbers in plaque and saliva specimens, including numbers for oral streptococci, and may be used as a potential assessment tool for oral hygiene and caries risk in children.

"The use of ATP-driven bioluminescence has broad implications in dentistry and medicine and can be used translationally in the clinic to determine the efficacy of interventional therapies, including the use of mouth rinses and perhaps in the detection of bacterial infections in periodontal and other infectious diseases," noted Curt Machida, Ph.D., principal investigator and OHSU professor of integrative biosciences and pediatric dentistry.

The OHSU team included Machida; Shahram Fazilat, D.D.S., a 2008 pediatric dentistry graduate; Rebecca Sauerwein, research assistant in integrative biosciences; Jennifer McLeod, a fourth-year dental student who recently graduated; Tyler Finlayson, second-year dental student; Emilia Adam, D.D.S., M.P.H., a second-year pediatric dentistry resident who recently graduated; John Engle, D.D.S., interim chair of the Department of Pediatric Dentistry; Prashant Gagneja, D.D.S., associate professor of pediatric dentistry; Tom Maier, Ph.D., assistant professor of integrative biosciences and oral pathology and radiology.

The research was funded by Oral BioTech, the OHSU School of Dentistry, and the Oregon Clinical and Translational Research Institute, which is funded by a grant from the National Center for Research Resources (NCRR), a component of the National Institutes of Health (NIH). Established in September 2006 through the NIH's Clinical and Translational Science Award (CTSA) program, OCTRI is a unique partnership between OHSU and Kaiser Permanente Center for Health Research -- bringing together an academic medical center and an innovative managed care organization. OCTRI's mission is to improve human health by enhancing clinical and translational research. OCTRI works with institutional partners, community organizations, and industry to engage communities in clinical research efforts and to reduce the time it takes for laboratory discoveries to become treatments for patients.


Story Source:

The above story is based on materials provided by Oregon Health & Science University. Note: Materials may be edited for content and length.


Journal Reference:

  1. Fazilat, Shahram; Sauerwein, Rebecca; Mcleod, Jennifer; Finlayson, Tyler; Adam, Emilia; Engle, John; Gagneja, Prashant; Maier, Tom; Machida, Curtis A. Application of Adenosine Triphosphate-Driven Bioluminescence for Quantification of Plaque Bacteria and Assessment of Oral Hygiene in Children. Pediatric Dentistry, Volume 32, Number 3, May / June 2010 , pp. 195-204(10) [link]

Cite This Page:

Oregon Health & Science University. "How to rapidly assess children's tooth decay risk." ScienceDaily. ScienceDaily, 2 July 2010. <www.sciencedaily.com/releases/2010/06/100622124708.htm>.
Oregon Health & Science University. (2010, July 2). How to rapidly assess children's tooth decay risk. ScienceDaily. Retrieved July 28, 2014 from www.sciencedaily.com/releases/2010/06/100622124708.htm
Oregon Health & Science University. "How to rapidly assess children's tooth decay risk." ScienceDaily. www.sciencedaily.com/releases/2010/06/100622124708.htm (accessed July 28, 2014).

Share This




More Health & Medicine News

Monday, July 28, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Traditional African Dishes Teach Healthy Eating

Traditional African Dishes Teach Healthy Eating

AP (July 28, 2014) Classes are being offered nationwide to encourage African Americans to learn about cooking fresh foods based on traditional African cuisine. The program is trying to combat obesity, heart disease and other ailments often linked to diet. (July 28) Video provided by AP
Powered by NewsLook.com
West Africa Gripped by Deadly Ebola Outbreak

West Africa Gripped by Deadly Ebola Outbreak

AFP (July 28, 2014) The worst-ever outbreak of the deadly Ebola epidemic grips west Africa, killing hundreds. Duration: 00:48 Video provided by AFP
Powered by NewsLook.com
Trees Could Save More Than 850 Lives Each Year

Trees Could Save More Than 850 Lives Each Year

Newsy (July 27, 2014) A national study conducted by the USDA Forest Service found that trees collectively save more than 850 lives on an annual basis. Video provided by Newsy
Powered by NewsLook.com
Google's Next Frontier: The Human Body

Google's Next Frontier: The Human Body

Newsy (July 27, 2014) Google is collecting genetic and molecular information to paint a picture of the perfectly healthy human. Video provided by Newsy
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:

Breaking News:
from the past week

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile: iPhone Android Web
Follow: Facebook Twitter Google+
Subscribe: RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins