Featured Research

from universities, journals, and other organizations

New method of tissue banking makes gene analysis more practical for lung cancer

Date:
July 16, 2010
Source:
International Association for the Study of Lung Cancer
Summary:
Analyzing the genes expressed by cancer cells allows for a better understanding of that patient's specific disease and in turn, a more personalized approach to treatment. But obtaining the RNA from a tumor in the lungs in order to conduct the genetic analysis is a challenging prospect. Currently, lung cancer researchers are limited to using RNA extracted from early-stage tumors removed during surgery.

Analyzing the genes expressed by cancer cells allows for a better understanding of that patient's specific disease and in turn, a more personalized approach to treatment. But obtaining the RNA from a tumor in the lungs in order to conduct the genetic analysis is a challenging prospect. Currently, lung cancer researchers are limited to using RNA extracted from early-stage tumors removed during surgery. The small quantities of tissue extracted during routine diagnostic biopsies have not been useful to researchers, due to their small size and the variety of ways they have been processed.

Related Articles


Since oftentimes surgery is not an option in advanced lung cancer, genetic analysis of the tumor is critical, there is a need to obtain good quality RNA samples from tumor tissue taken via biopsy, no matter how the biopsy procedure is conducted.

In a study in the July edition of the Journal of Thoracic Oncology, Malcolm H. Lawson describes a process to successfully obtain and to store high quality RNA from lung tumor biopsies. Lawson and fellow researchers at the Cancer Research UK Cambridge Research Institute and Papworth Hospital, both in the United Kingdom, received consent from patients to take extra biopsies for research purposes during the diagnostic procedure. Biopsies were obtained using the three most frequently used techniques -- endobronchial biopsy forceps, transbronchial needle aspirate -- or CT-guided needle biopsy. Acceptable RNA for gene expression analysis was extracted from 72 percent of lung cancer biopsies.

Lawson and team immediately froze some of the biopsies in liquid nitrogen, while the others were first treated with an RNA preservative (RNAlater) before freezing. Use of the RNA preservative resulted in higher quality, more intact RNA from biopsies taken via all methods.

Storage in the RNA preservative doubled the number of biopsies that met the minimum yield and quality criteria for analysis compared with immediate freezing (10 of 16 biopsies vs. 5 of 16). Using the RNA preservative, 70 percent of biopsies taken by needle aspiration were suitable for analysis compared with 40 percent frozen, and 50 percent of the RNA preservative endobronchial biopsies were acceptable compared with 17 percent of the frozen ones. A full 100 percent of the CT-guided needle biopsies met the criteria when treated with RNA preservative.

"This is a novel model for tissue banking lung cancer diagnostic specimens that greatly increases the number and type of tumors available for gene expression studies," Dr. Lawson said. "If used widely, it makes personalized medicine for lung cancer patients a more practical proposition."


Story Source:

The above story is based on materials provided by International Association for the Study of Lung Cancer. Note: Materials may be edited for content and length.


Journal Reference:

  1. Malcolm H. Lawson, Doris M. Rassl, Natalie M. Cummings, Roslin Russell, Jaymin B. Morjaria, James D. Brenton, Gillian Murphy, Robert C. Rintoul. Tissue Banking of Diagnostic Lung Cancer Biopsies for Extraction of High Quality RNA. Journal of Thoracic Oncology, 2010; 1 DOI: 10.1097/JTO.0b013e3181ddbbe9

Cite This Page:

International Association for the Study of Lung Cancer. "New method of tissue banking makes gene analysis more practical for lung cancer." ScienceDaily. ScienceDaily, 16 July 2010. <www.sciencedaily.com/releases/2010/07/100715123416.htm>.
International Association for the Study of Lung Cancer. (2010, July 16). New method of tissue banking makes gene analysis more practical for lung cancer. ScienceDaily. Retrieved December 19, 2014 from www.sciencedaily.com/releases/2010/07/100715123416.htm
International Association for the Study of Lung Cancer. "New method of tissue banking makes gene analysis more practical for lung cancer." ScienceDaily. www.sciencedaily.com/releases/2010/07/100715123416.htm (accessed December 19, 2014).

Share This


More From ScienceDaily



More Health & Medicine News

Friday, December 19, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

The Best Tips to Curb Holiday Carbs

The Best Tips to Curb Holiday Carbs

Buzz60 (Dec. 19, 2014) It's hard to resist those delicious but fattening carbs we all crave during the winter months, but there are some ways to stay satisfied without consuming the extra calories. Vanessa Freeman (@VanessaFreeTV) has the details. Video provided by Buzz60
Powered by NewsLook.com
Kids Die While Under Protective Services

Kids Die While Under Protective Services

AP (Dec. 18, 2014) As part of a six-month investigation of child maltreatment deaths, the AP found that hundreds of deaths from horrific abuse and neglect could have been prevented. AP's Haven Daley reports. (Dec. 18) Video provided by AP
Powered by NewsLook.com
Dads-To-Be Also Experience Hormone Changes During Pregnancy

Dads-To-Be Also Experience Hormone Changes During Pregnancy

Newsy (Dec. 18, 2014) A study from University of Michigan researchers found that expectant fathers see a decrease in testosterone as the baby's birth draws near. Video provided by Newsy
Powered by NewsLook.com
Prenatal Exposure To Pollution Might Increase Autism Risk

Prenatal Exposure To Pollution Might Increase Autism Risk

Newsy (Dec. 18, 2014) Harvard researchers found children whose mothers were exposed to high pollution levels in the third trimester were twice as likely to develop autism. Video provided by Newsy
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:

Breaking News:

Strange & Offbeat Stories


Health & Medicine

Mind & Brain

Living & Well

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile: iPhone Android Web
Follow: Facebook Twitter Google+
Subscribe: RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins