Featured Research

from universities, journals, and other organizations

Researchers use pyrosequencing to study canine intestinal bacteria

Date:
August 12, 2010
Source:
University of Illinois College of Agricultural, Consumer and Environmental Sciences
Summary:
A dog's indiscriminate taste is not always a positive trait. In fact, it often leads to gastrointestinal infections and consequent ailments such as diarrhea and vomiting that come from eating spoiled food. Others develop gastrointestinal diseases such as inflammatory bowel diseases that are not directly attributed to the diet, but are influenced by intestinal bacteria. Researchers at the University of Illinois are making strides in devising dietary interventions to combat these infections through advanced DNA pyrosequencing technology. Researchers at the University of Illinois are making strides in devising dietary interventions to combat these infections through advanced DNA pyrosequencing technology.

Researchers at the University of Illinois are making strides in devising dietary interventions to combat canine gastrointestinal infections through advanced DNA pyrosequencing technology.
Credit: Photo by Amy Fischer, University of Illinois

URBANA -- A dog's indiscriminate taste is not always a positive trait. In fact, it often leads to gastrointestinal infections and consequent ailments such as diarrhea and vomiting that come from eating spoiled food. Others develop gastrointestinal diseases such as inflammatory bowel diseases that are not directly attributed to the diet, but are influenced by intestinal bacteria.

Related Articles


Researchers at the University of Illinois are making strides in devising dietary interventions to combat these infections through advanced DNA pyrosequencing technology.

This new method of DNA sequencing has helped researchers uncover the phylogeny or "who's there" in a healthy dog's gut. Their goal was to obtain a standard that could be used as a comparison to diseased states in the future.

"It's a first step toward making progress in our understanding of how diet affects gastrointestinal infections," said Kelly Swanson, U of I associate professor of animal science. "Dogs do not rely heavily on microbial fermentation as it pertains to energy requirements, but a balanced and stable microbiota is critical for maintaining gastrointestinal health."

Up until now scientists could only culture a small percentage of the bacteria in a dog's gut and due to technological limitations, they couldn't determine the complete phylogeny.

More than 80,000 DNA sequences were evaluated in the first study to reveal that the highest number of bacteria present in the gut were Firmicutes and Bacteriodetes. Fusobacteria, while not as common, were also among the highest number of bacteria found.

"Often we don't know if an altered bacterial community is the cause of disease or the effect of disease," he said. "We also don't know if it's one species or multiple species working together to create problems for the dog's intestinal health."

In a follow-up study, using approximately one million DNA sequences, their team identified the metabolic pathways that exist in intestinal bacteria of healthy animals including nutrient metabolism, virulence, and stress response, Swanson said. They also compared the phylogeny of a dog's intestine to that of humans, mice and chickens to determine similarities and differences between species.

Swanson and his teammates are interested in continuing to learn how dietary intervention, pharmaceuticals and age affect microbial populations. In addition, most pet dogs in developed countries are now treated as family, with many not only living in the home, but also eating, sleeping and playing with their owners. This close proximity has relevance in regards to zoonotic disease and the direct link between human and dog illness.

"Phylogenetic characterization of fecal microbial communities of dogs fed diets with or without supplemental dietary fiber using 454 pyrosequencing" was published in PLoS ONE. Researchers include Ingmar Middelbos, Brittany Vester Boler, Ani Qu, Bryan White, George Fahey and Swanson, all of the U of I. Funding was provided by the Illinois Agricultural Experiment Station.

"Phylogenetic and gene-centric metagenomics of the canine intestinal microbiome reveals similarities with humans and mice" was published in The ISME Journal: Multidisciplinary Journal of Microbial Ecology. Researchers include Scot Dowd of Research and Testing Laboratory; Jan Suchodolski of Texas A& M University; Ingmar Middelbos, Brittany Vester Boler, Kathleen Barry, Isaac Cann, Bryan White, George Fahey and Swanson of the U of I; Karen Nelson and Manolito Torralba of the J. Craig Venter Institute; and Bernard Henrissat and Pedro Coutinho of the Universites Aix-Marseille I and II in France. Funding was provided by the Illinois Agricultural Experiment Station.


Story Source:

The above story is based on materials provided by University of Illinois College of Agricultural, Consumer and Environmental Sciences. The original article was written by Jennifer Shike. Note: Materials may be edited for content and length.


Journal Reference:

  1. Ingmar S. Middelbos, Brittany M. Vester Boler, Ani Qu, Bryan A. White, Kelly S. Swanson, George C. Fahey, Ching-Hong Yang. Phylogenetic Characterization of Fecal Microbial Communities of Dogs Fed Diets with or without Supplemental Dietary Fiber Using 454 Pyrosequencing. PLoS ONE, 2010; 5 (3): e9768 DOI: 10.1371/journal.pone.0009768

Cite This Page:

University of Illinois College of Agricultural, Consumer and Environmental Sciences. "Researchers use pyrosequencing to study canine intestinal bacteria." ScienceDaily. ScienceDaily, 12 August 2010. <www.sciencedaily.com/releases/2010/08/100812161938.htm>.
University of Illinois College of Agricultural, Consumer and Environmental Sciences. (2010, August 12). Researchers use pyrosequencing to study canine intestinal bacteria. ScienceDaily. Retrieved October 25, 2014 from www.sciencedaily.com/releases/2010/08/100812161938.htm
University of Illinois College of Agricultural, Consumer and Environmental Sciences. "Researchers use pyrosequencing to study canine intestinal bacteria." ScienceDaily. www.sciencedaily.com/releases/2010/08/100812161938.htm (accessed October 25, 2014).

Share This



More Plants & Animals News

Saturday, October 25, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Deep Sea 'mushroom' Could Be Early Branch on Tree of Life

Deep Sea 'mushroom' Could Be Early Branch on Tree of Life

Reuters - Innovations Video Online (Oct. 24, 2014) Miniature deep sea animals discovered off the Australian coast almost three decades ago are puzzling scientists, who say the organisms have proved impossible to categorise. Academics at the Natural History of Denmark have appealed to the world scientific community for help, saying that further information on Dendrogramma enigmatica and Dendrogramma discoides could answer key evolutionary questions. Jim Drury has more. Video provided by Reuters
Powered by NewsLook.com
Black Bear Cub Goes Sunday Shopping

Black Bear Cub Goes Sunday Shopping

Reuters - Light News Video Online (Oct. 23, 2014) Price check on honey? Bear cub startles Oregon drugstore shoppers. Rough Cut (no reporter narration). Video provided by Reuters
Powered by NewsLook.com
Dances With Wolves in China's Wild West

Dances With Wolves in China's Wild West

AFP (Oct. 23, 2014) One man is on a mission to boost the population of wolves in China's violence-wracked far west. The animal - symbol of the Uighur minority there - is under threat with a massive human resettlement program in the region. Duration: 00:41 Video provided by AFP
Powered by NewsLook.com
Breakfast Debate: To Eat Or Not To Eat?

Breakfast Debate: To Eat Or Not To Eat?

Newsy (Oct. 23, 2014) Conflicting studies published in the same week re-ignited the debate over whether we should be eating breakfast. Video provided by Newsy
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:

Breaking News:

Strange & Offbeat Stories


Plants & Animals

Earth & Climate

Fossils & Ruins

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile: iPhone Android Web
Follow: Facebook Twitter Google+
Subscribe: RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins