Featured Research

from universities, journals, and other organizations

Plant's light switch could be used to control cells

Date:
November 2, 2010
Source:
Duke University
Summary:
A scientists shines a blue light on yeast and mammalian cells in her lab and the edges of them start to glow. The effect is the result of a light-activated switch from a plant that has been inserted into the cell. Researchers could use this novel "on-off switch" to control cell growth or death, grow new tissue or deliver doses of medication directly to diseased cells.

Within milliseconds of a blue flash of light, a red fluorescent protein that typically sits in a cell (left) interacts with a plant protein attached to the cell membrane. The fluorescent protein then migrates to the cell's edge (right).
Credit: Chandra Tucker, Duke

Chandra Tucker shines a blue light on yeast and mammalian cells in her Duke University lab and the edges of them start to glow. The effect is the result of a light-activated switch from a plant that has been inserted into the cell.

Researchers could use this novel "on-off switch" to control cell growth or death, grow new tissue or deliver doses of medication directly to diseased cells, said Tucker, an assistant research professor in the biology department at Duke.

She and colleagues created the switch by genetically inserting two proteins from a mustard plant, Arabidopsis thaliana, into yeast cells, kidney cells and cultured rodent brain tissue. The two proteins interact under light to provide the control over cell functions.

The switch is similar to one described last year where researchers genetically inserted a different light-receptive plant protein and its interacting protein partner from Arabidopsis into mammalian cells. In response to red light, these proteins interacted to cause mammalian cells to change shape, moving in the direction of the light.

Tucker's switch uses Arabidopsis proteins that respond to blue light. Unlike the red-light activated proteins, which need an added cofactor, a chemical that is required for the light response, the blue-light switch doesn't need any additional chemicals to work because it uses a cofactor that naturally exists in non-plant organisms.

"It's hard to deliver a chemical to a fly or to individual cells. This new approach, with one of the molecules already in the mammalian or yeast cells, makes building a light-controlled switch a lot easier," Tucker said. Her team describes the switch in the Oct. 31 Nature Methods.

To test the switch, the team fused one of the light-sensitive Arabidopsis proteins to a red fluorescent protein and the other to a green fluorescent protein, which was in turn attached to the cell membrane. When the researchers flashed blue light on the cell, the plant proteins interacted, causing the red fluorescent protein to rapidly move to the cell membrane, which then glowed yellow due to the merging of the red and green fluorescing proteins. The team found that this interaction was reversible and could be triggered repeatedly with light exposure.

The switch is one among several that have been designed to give researchers better control of different functions of the cell. The next step in developing the switch will be to make the interacting proteins more effective, Tucker said. The approach is expected to be applicable not only for studies in cultured cells and yeast, but also worms, fruit flies, mice and other model organisms. Eventually this method could allow researchers to test how cells in a tissue affect neighboring cells in a tissue, to guide axon growth in neurons to repair brain tissue, or even to kill cancer cells.

Tucker's new approach will be a "major boon" to those who wish to apply light activation to their own experimental systems, said Klaus Hahn, a pharmacologist at the University of North Carolina at Chapel Hill, whose lab reported on another blue-light responsive protein to control movement of mammalian cells last year.

Hahn said the "elegant work will likely see broad use, in many fields and for applications that will surprise us," and it is already going to be applied to important areas of research, such as control of gene expression.


Story Source:

The above story is based on materials provided by Duke University. Note: Materials may be edited for content and length.


Journal Reference:

  1. Matthew J Kennedy, Robert M Hughes, Leslie A Peteya, Joel W Schwartz, Michael D Ehlers, Chandra L Tucker. Rapid blue-light–mediated induction of protein interactions in living cells. Nature Methods, 2010; DOI: 10.1038/nmeth.1524

Cite This Page:

Duke University. "Plant's light switch could be used to control cells." ScienceDaily. ScienceDaily, 2 November 2010. <www.sciencedaily.com/releases/2010/10/101031154011.htm>.
Duke University. (2010, November 2). Plant's light switch could be used to control cells. ScienceDaily. Retrieved July 23, 2014 from www.sciencedaily.com/releases/2010/10/101031154011.htm
Duke University. "Plant's light switch could be used to control cells." ScienceDaily. www.sciencedaily.com/releases/2010/10/101031154011.htm (accessed July 23, 2014).

Share This




More Plants & Animals News

Wednesday, July 23, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Stone Fruit Listeria Scare Causes Sweeping Recall

Stone Fruit Listeria Scare Causes Sweeping Recall

Newsy (July 22, 2014) The Wawona Packing Company has issued a voluntary recall on the stone fruit it distributes due to a possible Listeria outbreak. Video provided by Newsy
Powered by NewsLook.com
Michigan Plant's Goal: Flower and Die

Michigan Plant's Goal: Flower and Die

AP (July 22, 2014) An 80-year-old agave plant, which is blooming for the first and only time at a University of Michigan conservatory, will die when it's done (July 22) Video provided by AP
Powered by NewsLook.com
Huge Schizophrenia Study Finds Dozens Of New Genetic Causes

Huge Schizophrenia Study Finds Dozens Of New Genetic Causes

Newsy (July 22, 2014) The 83 new genetic markers could open dozens of new avenues for schizophrenia treatment research. Video provided by Newsy
Powered by NewsLook.com
CDC Head Concerned About a Post-Antibiotic Era

CDC Head Concerned About a Post-Antibiotic Era

AP (July 22, 2014) Sounding alarms about the growing threat of antibiotic resistance, CDC Director Tom Frieden warned Tuesday if the global community does not confront the problem soon, the world will be living in a devastating post-antibiotic era. (July 22) Video provided by AP
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:

Breaking News:
from the past week

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile: iPhone Android Web
Follow: Facebook Twitter Google+
Subscribe: RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins