Featured Research

from universities, journals, and other organizations

New method to quantify protein changes could advance study, treatment of various diseases including cancer

Date:
January 7, 2011
Source:
Scripps Research Institute
Summary:
New research has yielded a novel method of analyzing and quantifying changes in proteins that result from a common chemical process. The findings could provide new insights into the effects of a highly destructive form of stress on proteins in various disease models, particularly cancer.

A scientist from the Florida campus of The Scripps Research Institute has devised a new method of analyzing and quantifying changes in proteins that result from a common chemical process. The new findings could provide new insights into the effects of a highly destructive form of stress on proteins in various disease models, particularly cancer.

Related Articles


The study, published January 5, 2011, in the online Early View of the journal Angewandte Chemie, was designated by the journal as a "very important paper," a distinction bestowed on less than five percent of its publications.

"This new technique allows us to home in on which proteins are modified to a significant extent during periods of stress and how that changes during disease progression," said Kate Carroll, an associate professor in the Scripps Research Department of Chemistry who conducted the study with Young Ho Seo, a research fellow at The University of Michigan. "It gives us the chance to look more closely at targets for possible therapeutic intervention. From a practical standpoint, the technique is simple and will be accessible to biologists and chemists alike."

The new technique focuses on the process of cysteine S-hydroxylation, which plays a significant role in a number of events related to physiology in both health and disease, including the regulation of signaling proteins in various disease states.

The ability of the new technique to focus on signaling pathways, particularly in diseases such as cancer, is critical.

"Chronic disease states such as cancer can involve the modification of signaling proteins through S-hydroxylation, but other housekeeping proteins may also be targets," she said. "Key to distinguishing which of these proteins may be involved in pathogenesis is the ability to measure the amount of S-hydroxylation at specific sites within a protein. Now you'll be able to tell. This should help accelerate target identification in these disease-related signaling pathways and allow us to focus on proteins that are important to the process."

During periods of cellular stress, caused by factors such as exposure to UV radiation or many disease states, the level of highly reactive oxygen-containing molecules can increase, resulting in inappropriate modification of proteins and cell damage.

One oxidant, hydrogen peroxide, functions as a messenger that can activate cell proliferation through oxidation of cysteine residues in signaling proteins, producing sulfenic acid (i.e., S-hydroxylation); cysteine is an amino acid is synthesized in the body.

Extending the Gains of an Earlier Study

In a 2009 study, Carroll found that sulfenic acid served as an early warning biomarker of the reaction between hydrogen peroxide and cysteine. Carroll tagged the miniscule reaction target with a fluorescent dye antibody. With it, Carroll was able to read the levels of sulfenic acid levels in various cell lines, including breast cancer cells.

The new technique takes those findings several steps further by allowing scientists not only to quantify the modifications to various proteins, but also to monitor these changes at the level of individual cysteines within a single protein.

Carroll used a class of reagents called isotope-coded dimedone and iododimedone, which traps and tags sulfenic acids, allowing the cysteine sites and modified proteins to be easily identified. These probes, which are highly selective for sulfenic acid, allow the S-hydroxylation process to be monitored at the exact site of the modification.

The tagged proteins can be then be analyzed by mass spectrometry, a standard technology used to determine the precise make-up of proteins and other molecules.

"This technique should be widely accessible to the scientific community because it's so simple," Carroll said. "It should allow researchers to identify proteins with altered S-hydroxylation profiles whose function may lend insight into events in disease progression and have utility as potential markers for disease detection."


Story Source:

The above story is based on materials provided by Scripps Research Institute. Note: Materials may be edited for content and length.


Journal Reference:

  1. Young Ho Seo, Kate S. Carroll. Quantification of Protein Sulfenic Acid Modifications Using Isotope-Coded Dimedone and Iododimedone. Angewandte Chemie International Edition, 2011; DOI: 10.1002/anie.201007175

Cite This Page:

Scripps Research Institute. "New method to quantify protein changes could advance study, treatment of various diseases including cancer." ScienceDaily. ScienceDaily, 7 January 2011. <www.sciencedaily.com/releases/2011/01/110106091941.htm>.
Scripps Research Institute. (2011, January 7). New method to quantify protein changes could advance study, treatment of various diseases including cancer. ScienceDaily. Retrieved November 23, 2014 from www.sciencedaily.com/releases/2011/01/110106091941.htm
Scripps Research Institute. "New method to quantify protein changes could advance study, treatment of various diseases including cancer." ScienceDaily. www.sciencedaily.com/releases/2011/01/110106091941.htm (accessed November 23, 2014).

Share This


More From ScienceDaily



More Plants & Animals News

Sunday, November 23, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Anglerfish Rarely Seen In Its Habitat Will Haunt You

Anglerfish Rarely Seen In Its Habitat Will Haunt You

Newsy (Nov. 22, 2014) For the first time Monterey Bay Aquarium recorded a video of the elusive, creepy and rarely seen anglerfish. Video provided by Newsy
Powered by NewsLook.com
Birds Around the World Take Flight

Birds Around the World Take Flight

Reuters - Light News Video Online (Nov. 22, 2014) An imperial eagle equipped with a camera spreads its wings over London. It's just one of the many birds making headlines in this week's "animal roundup". Jillian Kitchener reports. Video provided by Reuters
Powered by NewsLook.com
Could Your Genes Be The Reason You're Single?

Could Your Genes Be The Reason You're Single?

Newsy (Nov. 21, 2014) Researchers in Beijing discovered a gene called 5-HTA1, and carriers are reportedly 20 percent more likely to be single. Video provided by Newsy
Powered by NewsLook.com
Raw: Baby Okapi Born at Houston Zoo

Raw: Baby Okapi Born at Houston Zoo

AP (Nov. 20, 2014) The Houston Zoo released video of a male baby okapi. Okapis, also known as the "forest giraffe", are native to the Democratic Republic of the Congo in Central Africa. Video is mute from source. (Nov. 20) Video provided by AP
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:

Breaking News:

Strange & Offbeat Stories


Plants & Animals

Earth & Climate

Fossils & Ruins

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile: iPhone Android Web
Follow: Facebook Twitter Google+
Subscribe: RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins