Featured Research

from universities, journals, and other organizations

Method of DNA repair linked to higher likelihood of genetic mutation

Date:
February 15, 2011
Source:
Public Library of Science
Summary:
Accurate transmission of genetic information requires the precise replication of DNA. Errors in DNA replication are common and nature has developed several cellular mechanisms for repairing these mistakes. Mutations, which can be deleterious (development of cancerous cells), or beneficial (evolutionary adaption), arise from uncorrected errors. Researchers report that a method by which cells repair breaks in their DNA, known as break-induced replication, is up to 2,800 times more likely to cause genetic mutation than normal DNA synthesis.

A chromosome's broken end invades an intact DNA molecule and initiates replication that can lead to a genomic instability.
Credit: Anna Malkova, Ph.D., School of Science at Indiana University-Purdue University Indianapolis

Accurate transmission of genetic information requires the precise replication of DNA. Errors in DNA replication are common and nature has developed several cellular mechanisms for repairing these mistakes. Mutations, which can be deleterious (development of cancerous cells), or beneficial (evolutionary adaption), arise from uncorrected errors.

Researchers from Indiana University-Purdue University Indianapolis (U.S.A) and Umea° University (Sweden) report that a method by which cells repair breaks in their DNA, known as Break-induced Replication (BIR), is up to 2,800 times more likely to cause genetic mutation than normal DNA synthesis. When one or many cells repair themselves using the efficient BIR method, accuracy is lost.

These findings will publish next week in the online, open access journal PLoS Biology.

"When BIR occurs, instead of using a "band aid" to repair a chromosomal break, the broken piece invades another chromosome and initiates replication which happens at the wrong place and at the wrong time and probably with participation of wrong proteins," said Anna Malkova, Ph.D., Associate Professor of Biology at the School of Science at IUPUI, who led the study.

The researchers used yeast to investigate the level of mutagenesis associated with BIR and found that the process's proclivity to cause mutation was not effected by where in the DNA the repair was made. But why is BIR so inaccurate as compared to normal replication?

"We didn't find a smoking gun," said Malkova, also an adjunct associate professor of medical and molecular genetics at the Indiana University School of Medicine. "We think there are at least four changes to the replication machinery that might occur to create a perfect storm or synergy that make BIR repair so mutagenic."

For example, during BIR, the researchers found a dramatic increase in the concentration of nucleotides -- the building blocks used to form DNA.

"Our findings strongly suggest that mutagenesis caused by BIR doesn't happen slowly, it occurs in surges -- sudden bursts which may lead to cancer," said Malkova, who is a geneticist. "We plan to continue investigating BIR in the hope of finding clues as to why this means of cell repair is so likely to cause mutations. The ultimate goal, of course, is to prevent those mutations that cause cancer."


Story Source:

The above story is based on materials provided by Public Library of Science. Note: Materials may be edited for content and length.


Journal Reference:

  1. Angela Deem, Andrea Keszthelyi, Tiffany Blackgrove, Alexandra Vayl, Barbara Coffey, Ruchi Mathur, Andrei Chabes, Anna Malkova. Break-Induced Replication Is Highly Inaccurate. PLoS Biology, 2011; 9 (2): e1000594 DOI: 10.1371/journal.pbio.1000594

Cite This Page:

Public Library of Science. "Method of DNA repair linked to higher likelihood of genetic mutation." ScienceDaily. ScienceDaily, 15 February 2011. <www.sciencedaily.com/releases/2011/02/110215191625.htm>.
Public Library of Science. (2011, February 15). Method of DNA repair linked to higher likelihood of genetic mutation. ScienceDaily. Retrieved August 20, 2014 from www.sciencedaily.com/releases/2011/02/110215191625.htm
Public Library of Science. "Method of DNA repair linked to higher likelihood of genetic mutation." ScienceDaily. www.sciencedaily.com/releases/2011/02/110215191625.htm (accessed August 20, 2014).

Share This




More Health & Medicine News

Wednesday, August 20, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Ebola-Hit Sierra Leone's Freetown a City on Edge

Ebola-Hit Sierra Leone's Freetown a City on Edge

AFP (Aug. 19, 2014) — Residents of Sierra Leone's capital voice their fears as the Ebola virus sweeps through west Africa. Duration: 00:56 Video provided by AFP
Powered by NewsLook.com
101-Year-Old Working Man Has All The Advice You Need

101-Year-Old Working Man Has All The Advice You Need

Newsy (Aug. 19, 2014) — Herman Goldman has worked at the same lighting store for almost 75 years. Find out his secrets to a happy, productive life. Video provided by Newsy
Powered by NewsLook.com
Researcher Testing on-Field Concussion Scanners

Researcher Testing on-Field Concussion Scanners

AP (Aug. 19, 2014) — Four Texas high school football programs are trying out an experimental system designed to diagnose concussions on the field. The technology is in response to growing concern over head trauma in America's most watched sport. (Aug. 19) Video provided by AP
Powered by NewsLook.com
American Ebola Patient Apparently Improving, Outbreak Is Not

American Ebola Patient Apparently Improving, Outbreak Is Not

Newsy (Aug. 19, 2014) — Nancy Writebol, an American missionary who contracted Ebola, is apparently getting better, according to her husband. The outbreak, however, is not. Video provided by Newsy
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
 
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:  

Breaking News:
from the past week

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:  

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile iPhone Android Web
Follow Facebook Twitter Google+
Subscribe RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins