Featured Research

from universities, journals, and other organizations

Entire T-cell receptor repertoire sequenced revealing extensive and unshared diversity

Date:
February 24, 2011
Source:
Cold Spring Harbor Laboratory
Summary:
T-cell receptor diversity in blood samples from healthy individuals has been extensively cataloged for the first time, setting the stage for a better understanding of infectious disease, cancer, and immune system disorders.

T-cell receptor diversity in blood samples from healthy individuals has been extensively cataloged for the first time in a study published online February 24 in Genome Research, setting the stage for a better understanding of infectious disease, cancer, and immune system disorders.

Related Articles


Adaptive immunity is mediated by T-cells, a white blood cell that identifies and attacks cells that may be infected with viruses or contain cancer-causing mutations. To recognize a wide array of potentially infectious agents or cancer-causing mutations, gene shuffling creates a highly variable and diverse collection of T-cell receptor sequences.

While the diversity of sequences in immune cell repertoires has been investigated previously, no study had yet been able to capture the entire range present in an individual sample. Now, using next-generation sequencing technology, researchers in Canada have identified essentially all T-cell receptor variants in blood samples, identifying more than one million unique sequences.

Dr. Robert Holt of the BC Cancer Agency and Simon Fraser University, senior author of the report, explained that this study is the first to establish that while there is high T-cell diversity in a standard blood sample, it does not give the entire picture. "This is only part of the diversity that would be present within a person's entire body," Holt said, "but now we know that although the diversity is very large, it is ultimately limited, and it is measureable."

The group found that some T-cell receptor sequences are common, some are rare, and the repertoire can change over time. The individual repertoire was then compared to that of two other individuals, showing that only a minority of sequences is shared between them.

Interestingly, they noted that for sequences that were shared, different gene shuffling events had often generated the same sequence. "This shows that certain sequences are more favored than others, most likely because they are more effective in recognizing specific types of infections or mutations," said Holt.

By cataloging the baseline diversity of the immune repertoire in a healthy individual, Holt explained that future studies would be able to then recognize how the repertoire is disturbed in cases of immune challenge, such as infectious disease or organ transplantation, and furthermore, may assist in the development of new vaccines.


Story Source:

The above story is based on materials provided by Cold Spring Harbor Laboratory. Note: Materials may be edited for content and length.


Journal Reference:

  1. Warren RL, Freeman JD, Zeng T, Choe G, Munro S, Moore R, Webb JR, Holt RA. Exhaustive T-cell repertoire sequencing of human peripheral blood samples reveals signatures of antigen selection and a directly measured repertoire size of at least 1 million clonotypes. Genome Res, February 24, 2011 DOI: 10.1101/gr.115428.110

Cite This Page:

Cold Spring Harbor Laboratory. "Entire T-cell receptor repertoire sequenced revealing extensive and unshared diversity." ScienceDaily. ScienceDaily, 24 February 2011. <www.sciencedaily.com/releases/2011/02/110223171232.htm>.
Cold Spring Harbor Laboratory. (2011, February 24). Entire T-cell receptor repertoire sequenced revealing extensive and unshared diversity. ScienceDaily. Retrieved January 29, 2015 from www.sciencedaily.com/releases/2011/02/110223171232.htm
Cold Spring Harbor Laboratory. "Entire T-cell receptor repertoire sequenced revealing extensive and unshared diversity." ScienceDaily. www.sciencedaily.com/releases/2011/02/110223171232.htm (accessed January 29, 2015).

Share This


More From ScienceDaily



More Health & Medicine News

Thursday, January 29, 2015

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Malnutrition on the Rise as Violence Flares in C. Africa

Malnutrition on the Rise as Violence Flares in C. Africa

AFP (Jan. 28, 2015) Violence can flare up at any moment in Bambari with only a bridge separating Muslims and Christians. Malnutrition is on the rise and lack of water means simple cooking fires threaten to destroy makeshift camps where people are living. Duration: 00:40 Video provided by AFP
Powered by NewsLook.com
Poultry Culled in Taiwan to Thwart Bird Flu

Poultry Culled in Taiwan to Thwart Bird Flu

Reuters - News Video Online (Jan. 28, 2015) Taiwan culls over a million poultry in efforts to halt various strains of avian flu. Julie Noce reports. Video provided by Reuters
Powered by NewsLook.com
Media Criticizing Parents For Not Vaccinating Children

Media Criticizing Parents For Not Vaccinating Children

Newsy (Jan. 28, 2015) As the Disneyland measles outbreak continues to spread, the media says parents who choose not to vaccinate their children are part of the cause. Video provided by Newsy
Powered by NewsLook.com
Shark Bite Victim Making Amazing Recovery

Shark Bite Victim Making Amazing Recovery

AP (Jan. 27, 2015) A Texas woman who lost more than five pounds of flesh to a shark in the Bahamas earlier this month could be released from a Florida hospital soon. Experts believe she was bitten by a bull shark while snorkeling. (Jan. 27) Video provided by AP
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:

Breaking News:

Strange & Offbeat Stories


Health & Medicine

Mind & Brain

Living & Well

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile: iPhone Android Web
Follow: Facebook Twitter Google+
Subscribe: RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins