Featured Research

from universities, journals, and other organizations

First stem cells from endangered species

Date:
September 5, 2011
Source:
Scripps Research Institute
Summary:
Starting with normal skin cells, scientists have produced the first stem cells from endangered species. Such cells could eventually make it possible to improve reproduction and genetic diversity for some species, possibly saving them from extinction, or to bolster the health of endangered animals in captivity.

Drill primate
Credit: San Diego Zoo

Starting with normal skin cells, scientists from The Scripps Research Institute have produced the first stem cells from endangered species. Such cells could eventually make it possible to improve reproduction and genetic diversity for some species, possibly saving them from extinction, or to bolster the health of endangered animals in captivity.

A description of the accomplishment appeared in an advance online edition of the journal Nature Methods on September 4, 2011.

Genesis

About five years ago, Oliver Ryder, PhD, the director of genetics at the San Diego Zoo Institute for Conservation Research, contacted Jeanne Loring, PhD, professor of developmental neurobiology at Scripps Research, to discuss the possibility of collecting stem cells from endangered species. Ryder's team had already established the Frozen Zoo, a bank of skin cells and other materials from more than 800 species and wondered if the thousands of samples they had amassed might be used as starting points.

Just as is hoped with humans, Ryder thought stem cells from endangered species might enable lifesaving medical therapies or offer the potential to preserve or expand genetic diversity by offering new reproduction possibilities.

At the time, although researchers were working with stem cells from embryos, scientists had not yet developed techniques for reliably inducing normal adult cells to become stem cells. But the technology arrived soon after, and scientists now accomplish this feat, called induced pluripotency, by inserting genes in normal cells that spark the transformation.

While Loring's team met with Ryder in early 2008, they realized that these newly emerging techniques might be applied to endangered species. Postdoctoral fellow Inbar Friedrich Ben-Nun, PhD, set out to systematically explore the possibilities.

Ryder suggested two species for initial work. The first was a highly endangered primate called a drill that he chose because of its close genetic connection to humans, and because in captivity the animals often suffer from diabetes, which researchers are working to treat in humans using stem cell-based therapies.

The northern white rhinoceros was the second candidate. Ryder chose this animal because it is genetically far removed from primates, and because it is one of the most endangered species on the planet. There are only seven animals still in existence, two of which reside at the San Diego Zoo Safari Park.

Initially members of the team thought they would have to isolate and use genes from animals closely related to the endangered species to successfully induce pluripotency. But that line of experimentation didn't work. Instead, to their surprise, after a year of trial and error, the researchers found that the same genes that induce pluripotency in humans also worked for the drill and the rhino. "It has been just amazing," said Ryder of the Scripps Research team's successes.

The process is inefficient, meaning only a few stem cells are produced at a time, but that's enough. "There are only two animals in it," said Ben-Nun, "but we have the start of a new zoo, the stem cell zoo."

Stem Cells to the Rescue

The scientists view their success as a first step toward greater advancements. Besides the possibility of using stem cells as the basis for diabetes or other treatments, there is great potential for new reproductive technologies as the stem cell research field advances. "The most important thing is to provide these stem cells as a resource for other people taking some of the next steps," said Loring.

One of the greatest concerns with small populations such as the northern white rhinos is that even if they did reproduce, which hasn't happened in many years, their genetic diversity is inevitably and dangerously low, and such inbreeding leads to unhealthy animals.

But researchers are moving toward inducing stem cells to differentiate into sperm or egg cells. With that accomplished, one possibility is that scientists could take skin cells in the Frozen Zoo from long dead animals, induce pluripotency, trigger differentiation into sperm cells, and then combine these with a living animal's eggs through in vitro fertilization. Otherwise-lost genetic diversity would then be reintroduced into the population, making it healthier, larger, and more robust.

Or, both eggs and sperm might be produced from the stem cells, with the resulting embryos implanted in live animals, a process that current research suggests could be much more reliable than existing cloning techniques.

Scientists are already exploring the possibility of producing sperm and eggs from stem cells as a potential solution to human infertility issues. Loring hopes that some of these groups might consider initial technique development using endangered species stem cells. "I think that work would be a lot easier ethically with endangered species than with humans," she said, "so I suspect some people working in this area would love to have our cells for experiments."

The Real Solution

"The best way to manage extinctions is to preserve species and their habitats," said Ryder, "but that's not working all the time." The rhinos are a perfect example, he said, because there are so few. "Stem cell technology provides some level of hope that they won't have to become extinct even though they've been completely eliminated from their habitats. I think that if humankind wants to save this species, we're going to have to develop new methodologies."

And even when there are reasonable wild populations of a species, they face a range of threats, including loss of habitat and poaching.

Moving forward, Loring said the group is hoping to continue producing stem cells from other species to expand their fledgling stem cell "zoo." For now, they're working to secure funding for what amounts to an unconventional line of research. "It's in between fields," said Loring. "It's not classical conservation and it's not ordinary biological research."

This research was supported by the Esther O'Keefe Foundation, the Millipore Foundation, and the California Institute for Regenerative Medicine.


Story Source:

The above story is based on materials provided by Scripps Research Institute. Note: Materials may be edited for content and length.


Journal Reference:

  1. Inbar Friedrich Ben-Nun, Susanne C Montague, Marlys L Houck, Ha T Tran, Ibon Garitaonandia, Trevor R Leonardo, Yu-Chieh Wang, Suellen J Charter, Louise C Laurent, Oliver A Ryder, Jeanne F Loring. Induced pluripotent stem cells from highly endangered species. Nature Methods, 2011; DOI: 10.1038/nmeth.1706

Cite This Page:

Scripps Research Institute. "First stem cells from endangered species." ScienceDaily. ScienceDaily, 5 September 2011. <www.sciencedaily.com/releases/2011/09/110904140411.htm>.
Scripps Research Institute. (2011, September 5). First stem cells from endangered species. ScienceDaily. Retrieved August 27, 2014 from www.sciencedaily.com/releases/2011/09/110904140411.htm
Scripps Research Institute. "First stem cells from endangered species." ScienceDaily. www.sciencedaily.com/releases/2011/09/110904140411.htm (accessed August 27, 2014).

Share This




More Plants & Animals News

Wednesday, August 27, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Raw: Firefighters Rescue Puppy Stuck in Tire

Raw: Firefighters Rescue Puppy Stuck in Tire

AP (Aug. 26, 2014) It took Houston firefighters more than an hour to free a puppy who got its head stuck in a tire. (Aug. 26) Video provided by AP
Powered by NewsLook.com
Have You Ever Been 'Sleep Drunk?' 1 in 7 Has

Have You Ever Been 'Sleep Drunk?' 1 in 7 Has

Newsy (Aug. 26, 2014) A study published in the journal "Neurology" interviewed more than 19,000 people and found 15 percent suffer from being "sleep drunk." Video provided by Newsy
Powered by NewsLook.com
Great White Shark Spotted Off Massachusetts Coast

Great White Shark Spotted Off Massachusetts Coast

Reuters - US Online Video (Aug. 26, 2014) A great white shark is spotted off the shore at Duxbury beach in Massachusetts forcing beach goers out of the water. Linda So reports. Video provided by Reuters
Powered by NewsLook.com
Raw: Elk Wanders Into German Office Building

Raw: Elk Wanders Into German Office Building

AP (Aug. 25, 2014) A young bull elk wandered inside the office building of a company in Dresden, Germany on Monday. The elk became trapped between a wall and glass windows while rescue workers tried to rescue him safely. (Aug. 25) Video provided by AP
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:

Breaking News:
from the past week

In Other News

... from NewsDaily.com

Science News

Health News

    Environment News

    Technology News



    Save/Print:
    Share:

    Free Subscriptions


    Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

    Get Social & Mobile


    Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

    Have Feedback?


    Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
    Mobile: iPhone Android Web
    Follow: Facebook Twitter Google+
    Subscribe: RSS Feeds Email Newsletters
    Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins