Featured Research

from universities, journals, and other organizations

Quantitative imaging application to gut and ear cells

Date:
January 15, 2012
Source:
Brigham and Women's Hospital
Summary:
From tracking activities within bacteria to creating images of molecules that make up human hair, several experiments have already demonstrated the unique abilities of the revolutionary imaging technique called multi-isotope imaging mass spectometry, or MIMS. MIMS can produce high-resolution, quantitative three-dimensional images of stable isotope tags within subcellular compartments in tissue sections or cells.

From tracking activities within bacteria to creating images of molecules that make up human hair, several experiments have already demonstrated the unique abilities of the revolutionary imaging technique called multi-isotope imaging mass spectometry, or MIMS, developed by researchers at Brigham and Women's Hospital (BWH). MIMS can produce high-resolution, quantitative three-dimensional images of stable isotope tags within subcellular compartments in tissue sections or cells.

Related Articles


With its use of stable isotopes as tracers, MIMS has opened the door for biomedical researchers to answer various biological questions, as two new studies have demonstrated. These studies looked at the use of MIMS in tracking cell division in intestinal stem cells, lipid turnover in Drosophila flies, protein turnover in ear cells, and opened the way to human application by detecting the formation of new white blood cells.

Both studies were published in Nature online on January 15, 2012 and will be in print on January 26, 2012.

In the first study, researchers used MIMS to test the much debated "immortal strand hypothesis" which claims that as stem cells divide, the older template DNA remains together in a stem cell, as the newer DNA is passed to cells that differentiate forming the digestive lining of the small intestine.

By tagging DNA with stable isotope tracers, researchers tracked DNA replication as cells divided. They found that in any situation DNA segregation was random, thereby disproving the immortal strand hypothesis.

The research opened another door by studying lipid metabolism within single lipid droplets of the fat body and of the central nervous system of Drosophila larvae. The researchers were also able to translate their work to humans. In a pilot study, they used MIMS to successfully track the formation of new white blood cells after administering isotope tracers in a healthy human volunteer.

The second study demonstrated that protein turnover in stereocilia in the inner ear is extremely slow contrary to the prevalent belief in the field. Stereocilia are hair-like projections found in cells of the inner ear that are responsible for hearing and maintaining balance. Using MIMS, researchers saw that protein turnover was very slow throughout the stereocilia, except the tip at the location of the mechanoelectrical transduction apparatus.

MIMS was created by developing several tools -- an ion microscope/secondary-ion mass spectrometer, labeling with stable isotopes, and quantitative image-analysis software. Unlike other imaging technologies, MIMS does not require staining or the use of radioactive labeling. MIMS enables researchers to conduct experiments with safe, non-toxic stable isotopes, which are naturally occurring components of all living matter.

Claude Lechene, MD, professor in the Division of Genetics, Department of Medicine and director of the National Resource for Imaging Mass Spectrometry (National Institutes of Health), was the senior study author for both studies.


Story Source:

The above story is based on materials provided by Brigham and Women's Hospital. Note: Materials may be edited for content and length.


Journal References:

  1. Duan-Sun Zhang, Valeria Piazza, Benjamin J. Perrin, Agnieszka K. Rzadzinska, J. Collin Poczatek, Mei Wang, Haydn M. Prosser, James M. Ervasti, David P. Corey, Claude P. Lechene. Multi-isotope imaging mass spectrometry reveals slow protein turnover in hair-cell stereocilia. Nature, 2012; DOI: 10.1038/nature10745
  2. Matthew L. Steinhauser, Andrew P. Bailey, Samuel E. Senyo, Christelle Guillermier, Todd S. Perlstein, Alex P. Gould, Richard T. Lee, Claude P. Lechene. Multi-isotope imaging mass spectrometry quantifies stem cell division and metabolism. Nature, 2012; DOI: 10.1038/nature10734

Cite This Page:

Brigham and Women's Hospital. "Quantitative imaging application to gut and ear cells." ScienceDaily. ScienceDaily, 15 January 2012. <www.sciencedaily.com/releases/2012/01/120115140055.htm>.
Brigham and Women's Hospital. (2012, January 15). Quantitative imaging application to gut and ear cells. ScienceDaily. Retrieved October 25, 2014 from www.sciencedaily.com/releases/2012/01/120115140055.htm
Brigham and Women's Hospital. "Quantitative imaging application to gut and ear cells." ScienceDaily. www.sciencedaily.com/releases/2012/01/120115140055.htm (accessed October 25, 2014).

Share This



More Health & Medicine News

Saturday, October 25, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Texas Nurse Nina Pham Cured of Ebola

Texas Nurse Nina Pham Cured of Ebola

AFP (Oct. 25, 2014) — An American nurse who contracted Ebola while caring for a Liberian patient in Texas has been declared free of the virus and will leave the hospital. Duration: 01:01 Video provided by AFP
Powered by NewsLook.com
IKEA Desk Converts From Standing to Sitting With One Button

IKEA Desk Converts From Standing to Sitting With One Button

Buzz60 (Oct. 24, 2014) — IKEA is out with a new convertible desk that can convert from a sitting desk to a standing one with just the push of a button. Jen Markham explains. Video provided by Buzz60
Powered by NewsLook.com
Ebola Protective Suits Being Made in China

Ebola Protective Suits Being Made in China

AFP (Oct. 24, 2014) — A factory in China is busy making Ebola protective suits for healthcare workers and others fighting the spread of the virus. Duration: 00:38 Video provided by AFP
Powered by NewsLook.com
WHO: Millions of Ebola Vaccine Doses by 2015

WHO: Millions of Ebola Vaccine Doses by 2015

AP (Oct. 24, 2014) — The World Health Organization said on Friday that millions of doses of two experimental Ebola vaccines could be ready for use in 2015 and five more experimental vaccines would start being tested in March. (Oct. 24) Video provided by AP
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
 
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:  

Breaking News:

Strange & Offbeat Stories

 

Health & Medicine

Mind & Brain

Living & Well

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:  

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile iPhone Android Web
Follow Facebook Twitter Google+
Subscribe RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins