Max Planck researchers reveal the structure of the cellular protein degradation machinery. Defective proteins that are not disposed of by the body can cause diseases such as Alzheimer's or Parkinson's. Scientists at the Max Planck Institute (MPI) of Biochemistry recently succeeded in revealing the structure of the cellular protein degradation machinery (26S proteasome) by combining different methods of structural biology. The results of collaboration with colleagues from the University of California, San Francisco and the Swiss Federal Institute of Technology Zurich (ETH Zürich) represent an important step forward in the investigation of the 26S proteasome.
The findings have now been published in the Proceedings of the National Academy of Sciences.
At any given point in time, cells may contain only the proteins that are needed at exactly this moment. Otherwise, undesirable reactions can occur which could cause cancer or other diseases. Furthermore, the proteins have to be folded correctly to fulfill their tasks. Misfolded proteins can clump into aggregates, and neurodegenerative diseases such as Alzheimer's or Parkinson's may be the consequence. In order to prevent this, several mechanisms in the body regulate the number of proteins in the cell and degrade proteins if necessary.
"Cellular waste disposal" -- the 26S proteasome -- plays an important role in protein degradation. First, misfolded and potentially dangerous proteins are tagged with molecules called ubiquitin. The 26S proteasome detects the tagged proteins and breaks them down into small fragments, which are then recycled. Scientists in the team of Wolfgang Baumeister, head of the research department "Molecular Structural Biology" at the MPI of Biochemistry, have now been able to reveal its structure.
Many puzzle pieces lead to one structure
"The structure of the 26S proteasome changes continuously," explained Friedrich Förster, head of the research group "Modeling of Protein Complexes" at the MPI of Biochemistry. "That is why until now it could not be explained by means of traditional approaches, such as only using X-ray crystallography. We had to combine different methods to be successful." Electron microscopy and mass spectrometry helped to reveal the general structure of the 26S proteasome. X-ray crystallography provided detailed insights into specific areas of the molecule. The researchers then used computer software to integrate the different data and generate an overall picture.
Based on these results, the researchers next want to find out how the different mechanisms of protein degradation work in detail. "We have already developed a hypothesis of how exactly the 26S proteasome detects tagged proteins and processes them," said Stefan Bohn, scientist at the MPI of Biochemistry. The complete elucidation of the 26S proteasome and its underlying mechanisms could also be of medical importance: "Cellular waste disposal" is a therapeutic target for cancer und neurodegenerative diseases.
- Keren Lasker, Friedrich Förster, Stefan Bohn, Thomas Walzthoeni, Elizabeth Villa, Pia Unverdorben, Florian Beck, Ruedi Aebersold, Andrej Sali, Wolfgang Baumeister. Molecular architecture of the 26S proteasome holocomplex determined by an integrative approach. Proceedings of the National Academy of Sciences, 2012; DOI: 10.1073/pnas.1120559109
- G. R. Pathare, I. Nagy, S. Bohn, P. Unverdorben, A. Hubert, R. Korner, S. Nickell, K. Lasker, A. Sali, T. Tamura, T. Nishioka, F. Forster, W. Baumeister, A. Bracher. The proteasomal subunit Rpn6 is a molecular clamp holding the core and regulatory subcomplexes together. Proceedings of the National Academy of Sciences, 2011; 109 (1): 149 DOI: 10.1073/pnas.1117648108
- E. Sakata, S. Bohn, O. Mihalache, P. Kiss, F. Beck, I. Nagy, S. Nickell, K. Tanaka, Y. Saeki, F. Forster, W. Baumeister. Localization of the proteasomal ubiquitin receptors Rpn10 and Rpn13 by electron cryomicroscopy. Proceedings of the National Academy of Sciences, 2012; DOI: 10.1073/pnas.1119394109
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