Featured Research

from universities, journals, and other organizations

Micro-copier for genome analysis

Date:
July 31, 2012
Source:
Albert-Ludwigs-Universität Freiburg
Summary:
A new method holds promise to advance personalized medicine. Scientists can now copy 100,000 different DNA sequences simultaneously in a so called picowell array the size of a one cent coin.

The DNA sequences are replicated in picowell arrays (bottom) and transferred on microscopic slides (top).
Credit: IMTEK, Laboratory for MEMS Applications

A new method holds promise to advance personalized medicine.

The scientists Jochen Hoffmann, Dr. Guenther Roth, and Prof. Dr. Roland Zengerle from the Department of Microsystems Engineering (IMTEK) at the University of Freiburg  can now copy 100,000 different DNA sequences simultaneously  in a so called picowell array the size of a one cent coin.

The picowell array is a chip with 100,000 wells on which the DNA sequences are dispersed in order to achieve a statistical distribution of one sequence per well. All wells are then covered with one microscopic slide and the DNA sequences are replicated by polymerase chain reaction (PCR) technique. The resulting DNA copies bind to the miscroscopic slide at exactly the position of the original DNA sequence. The DNA copies can be decoded by next-generation sequencing systems in the wells of the array. The microscopic slides can be used, similar to DNA microarrays, for the rapid and cost-effective analysis of entire genomes -- without the need for other equipment or additional transfer steps.

Such genome analyses allow insights into the regulatory systems of an organism. For example the genome analysis of a tumour cell can reveal which of its signal paths are defective. This knowledge in the context of "personalised medicine" can help to develop a tailored therapy for a specific patient group.

The project was supported by the "Program for Excellency in Science and Technology" as part of a grant from the Hans L- Merkle-Stiftung. The Robert Bosch GmbH and the Association for the Promotion of Science and Humanities in Germany also supported the project.

The topic is now further developed in the Lab-on-a-Chip research groups "Arrays" of Jochen Hoffmann and "Assays" of Dr. Guenther Roth. Lab-on-a-chip is a main research topic in cooperation with the Laboratory for MEMS Applications and the Institut fuer Mikro- und Informationstechnik der Hahn-Schickard-Gesellschaft (HSG-IMIT). The research groups are based on the campus of the Faculty for Engineering.


Story Source:

The above story is based on materials provided by Albert-Ludwigs-Universität Freiburg. Note: Materials may be edited for content and length.


Journal Reference:

  1. Jochen Hoffmann, Martin Trotter, Felix von Stetten, Roland Zengerle, Günter Roth. Solid-phase PCR in a picowell array for immobilizing and arraying 100 000 PCR products to a microscope slide. Lab on a Chip, 2012; DOI: 10.1039/C2LC40534B

Cite This Page:

Albert-Ludwigs-Universität Freiburg. "Micro-copier for genome analysis." ScienceDaily. ScienceDaily, 31 July 2012. <www.sciencedaily.com/releases/2012/07/120731094617.htm>.
Albert-Ludwigs-Universität Freiburg. (2012, July 31). Micro-copier for genome analysis. ScienceDaily. Retrieved July 31, 2014 from www.sciencedaily.com/releases/2012/07/120731094617.htm
Albert-Ludwigs-Universität Freiburg. "Micro-copier for genome analysis." ScienceDaily. www.sciencedaily.com/releases/2012/07/120731094617.htm (accessed July 31, 2014).

Share This




More Matter & Energy News

Thursday, July 31, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Britain Testing Driverless Cars on Roadways

Britain Testing Driverless Cars on Roadways

AP (July 30, 2014) — British officials said on Wednesday that driverless cars will be tested on roads in as many as three cities in a trial program set to begin in January. Officials said the tests will last up to three years. (July 30) Video provided by AP
Powered by NewsLook.com
Amid Drought, UCLA Sees Only Water

Amid Drought, UCLA Sees Only Water

AP (July 30, 2014) — A ruptured 93-year-old water main left the UCLA campus awash in 8 million gallons of water in the middle of California's worst drought in decades. (July 30) Video provided by AP
Powered by NewsLook.com
Smartphone Powered Paper Plane Debuts at Airshow

Smartphone Powered Paper Plane Debuts at Airshow

AP (July 30, 2014) — Smartphone powered paper airplane that was popular on crowdfunding website KickStarter makes its debut at Wisconsin airshow (July 30) Video provided by AP
Powered by NewsLook.com
U.K. To Allow Driverless Cars On Public Roads

U.K. To Allow Driverless Cars On Public Roads

Newsy (July 30, 2014) — Driverless cars could soon become a staple on U.K. city streets, as they're set to be introduced to a few cities in 2015. Video provided by Newsy
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
 
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:  

Breaking News:
from the past week

In Other News

... from NewsDaily.com

Science News

Health News

    Environment News

    Technology News



    Save/Print:
    Share:  

    Free Subscriptions


    Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

    Get Social & Mobile


    Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

    Have Feedback?


    Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
    Mobile iPhone Android Web
    Follow Facebook Twitter Google+
    Subscribe RSS Feeds Email Newsletters
    Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins