Novel technique demonstrates interactions between malaria parasite and HIV

The study is led by Dr. David Richard of the Centre Hospitalier Universitaire de Quebec (CHUQ). Dr. Richard explains, "We don't know much about what is happening at the cellular level when HIV-1-infected immune cells encounter the malaria parasite. Results obtained from the few studies exploring the interaction of these two diseases are sometimes conflicting. We hope that our model will allow us to thoroughly dissect these interactions in a simplified system."

Each disease attacks a different component of human blood, thus disturbing normal immune function. P. falciparum infect red blood cells and cause fever, shivering, vomiting, or convulsions in patients. HIV-1 causes acquired immune deficiency syndrome (AIDS) by infecting components of the immune system, including macrophages and helper T cells, and then replicates and destroys the host cells. By studying co-infection at different phases of each disease in vitro, scientists can better understand how different stages of malaria infection and HIV reproduction affect the onset and severity of the other disease. As such, Dr. Richard and his laboratory present a technique that investigates how P. falciparum-infected red blood cells affect the replication of HIV-1 in monocyte-derived macrophages.

Dr. Richard points out that, "by publishing in JoVE, you really see what is happening in the experiment. The visual representation helps succinctly explain a long procedure, and gives you a fuller picture of the schematic complexity." He hopes that this publication will give the scientific community the tools to look at the interactions on a cellular level, which would be an initial step in improving the quality of life for individuals infected by these deadly diseases. "This protocol provides a tool to examine the interactions between P. falciparium and HIV," states JoVE editor Dr. Charlotte Frank Sage, "Publication of the protocol in JoVE will allow researchers around the world to see a detailed demonstration of this system which will help in bring the technology to their laboratories."