Featured Research

from universities, journals, and other organizations

Using novel genetic labs for faster detection of E. coli

Date:
December 20, 2012
Source:
Frontiers
Summary:
Pina Fratamico is on the quest to find the easiest and fastest way to test for harmful Escherichia coli in ground beef. In a new article, she explores using a next-generation real-time polymerase chain reaction (PCR) system to discover specific gene targets that indicate the presence of dangerous foodborne pathogens. The results show that assays performed using this PCR system are rapid, sensitive, and reliable.

Pina Fratamico is on the quest to find the easiest and fastest way to test for harmful Escherichia coli in ground beef. In an article published Dec. 20 in Frontiers in Microbiology, she explores using a next-generation real-time polymerase chain reaction (PCR) system to discover specific gene targets that indicate the presence of dangerous foodborne pathogens. The results show that assays performed using this PCR system are rapid, sensitive, and reliable.

Related Articles


"Testing using these types of systems is faster, easier, and more reproducible than previous methods, and this should increase food safety in the long run. I feel that we could confidently move to these new systems for screening ground beef and other foods for E. coli contamination," says Fratamico, researcher at the USDA Agricultural Research Service in Wyndmoor, Pennsylvania.

Not all E. coli are dangerous, but certain strains produce a potentially dangerous toxin called Shiga toxin. These Shiga toxin-producing E. coli also known as STEC can be found in raw meat and cause serious food poisoning in humans. According the Food Safety and Inspection Service (FSIS) website, in October of this year over, 2,300 pounds of ground beef were recalled due to contamination with STEC.

"Certain groups of STEC have been declared as adulterants by the USDA FSIS, and the availability of rapid and reliable tests for these pathogens is critical so that testing results are available before meat is shipped to restaurants and consumers," explains Fratamico.

The PCR protocol has already been used for some time in the meat industry. The genetic test detects the presence of specific gene targets that indicate the existence of STEC in meat. The new generation of real-time PCR systems, like the GeneDisc from Pall Technologies in France used in this particular study, employ a self-contained unit that standardizes the procedure and tend to be relatively portable and easy to use -- offering obvious advantages for both meat processors and inspectors from the industry and government alike.


Story Source:

The above story is based on materials provided by Frontiers. Note: Materials may be edited for content and length.


Journal Reference:

  1. Pina M. Fratamico, Lori K. Bagi. Detection of Shiga toxin-producing Escherichia coli in ground beef using the GeneDisc real-time PCR system. Frontiers in Cellular and Infection Microbiology, 2012; 2 DOI: 10.3389/fcimb.2012.00152

Cite This Page:

Frontiers. "Using novel genetic labs for faster detection of E. coli." ScienceDaily. ScienceDaily, 20 December 2012. <www.sciencedaily.com/releases/2012/12/121220080449.htm>.
Frontiers. (2012, December 20). Using novel genetic labs for faster detection of E. coli. ScienceDaily. Retrieved March 2, 2015 from www.sciencedaily.com/releases/2012/12/121220080449.htm
Frontiers. "Using novel genetic labs for faster detection of E. coli." ScienceDaily. www.sciencedaily.com/releases/2012/12/121220080449.htm (accessed March 2, 2015).

Share This


More From ScienceDaily



More Plants & Animals News

Monday, March 2, 2015

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Whale-Watching Scientists Spot Baby Orca

Whale-Watching Scientists Spot Baby Orca

AP (Feb. 28, 2015) Researchers following endangered killer whales spotted a baby orca off the coast of Washington state, the third birth documented this winter but still leaving the population dangerously low. (Feb. 28) Video provided by AP
Powered by NewsLook.com
The Best Drinks for Your Health

The Best Drinks for Your Health

Buzz60 (Feb. 27, 2015) When it comes to health and fitness, there&apos;s lots of talk about what foods to eat, but there are a few liquids that can promote good nutrition. Krystin Goodwin (@krystingoodwin) has the healthiest drinks to boost your health! Video provided by Buzz60
Powered by NewsLook.com
Cherries, Snap Peas and More Tasty Spring Produce

Cherries, Snap Peas and More Tasty Spring Produce

Buzz60 (Feb. 27, 2015) From sweet cherries to sugar snap peas, spring is the peak season for some of the tastiest and healthiest produce. Krystin Goodwin (@Krystingoodwin) has the best seasonal fruits and veggies to spring in to good health! Video provided by Buzz60
Powered by NewsLook.com
The Best Foods to Battle Stress

The Best Foods to Battle Stress

Buzz60 (Feb. 26, 2015) If you&apos;re dealing with anxiety, there are a few foods that can help. Krystin Goodwin (@krystingoodwin) has the best foods to tame stress. Video provided by Buzz60
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:

Breaking News:

Strange & Offbeat Stories


Plants & Animals

Earth & Climate

Fossils & Ruins

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile: iPhone Android Web
Follow: Facebook Twitter Google+
Subscribe: RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins