Featured Research

from universities, journals, and other organizations

Pour, shake and stir: How gold particles, DNA and water have the potential to shape the future of medicine

Date:
February 28, 2013
Source:
University of Toronto Faculty of Applied Science & Engineering
Summary:
A diagnostic "cocktail" containing a single drop of blood, a dribble of water, and a dose of DNA powder with gold particles could mean rapid diagnosis and treatment of the world's leading diseases in the near future.

IBBME PhD student Kyryl Zagorovsky and Professor Warren Chan.
Credit: Image courtesy of University of Toronto Faculty of Applied Science & Engineering

A diagnostic 'cocktail,' containing a single drop of blood, a dribble of water and a dose of DNA powder with gold particles, could one day lead to the treatment of the world's leading diseases.

Related Articles


This homegrown brew is being developed by IBBME PhD student Kyryl Zagorovsky and Professor Warren Chan (IBBME), a Canada Research Chair in Nanobiotechnology, and a recent winner of the NSERC E.W.R. Steacie Memorial Fellowship.

"There's been a lot of emphasis in developing simple diagnostics," said Professor Chan. "The question is, how do you make it simple enough, portable enough?"

Professor Chan's lab studies nanoparticles -- in particular, the use of gold particles in sizes so small that they are measured in the nanoscale. He and his group are working on custom-designing nanoparticles to target and illuminate cancer cells and tumours, with the potential of one day being able to deliver drugs to cancer cells.

Zagorovsky's rapid-diagnostic biosensor will allow technicians to test for multiple diseases at one time with one small sample, and with high accuracy and sensitivity. The biosensor relies upon gold particles in much the same vein as your average pregnancy test. With a pregnancy test, gold particles turn the test window red because the particles are linked with an antigen that detects a certain hormone in the urine of a pregnant woman.

"Gold is the best medium," explained Professor Chan, "because it's easy to see. It emits a very intense colour."

Currently, scientists can target a particular disease by linking gold particles with DNA strands. When a sample containing the disease gene (e.g., Malaria) is present, it clumps the gold particles, turning the sample blue.

Rather than clumping the particles together, Zagorovsky immerses the gold particles in a DNA-based enzyme solution (DNA-zyme) that, when the disease gene is introduced, 'snip' the DNA from the gold particles, turning the sample red.

"It's like a pair of scissors," said Zagorovsky. "The target gene activates the scissors that cut the DNA links holding gold particles together."

The advantage is that far less of the gene needs to be present for the solution to show noticeable colour changes, amplifying detection. A single DNA-zyme can clip up to 600 'links' between the target genes.

Just a single drop from a biological sample such as saliva or blood can potentially be tested in parallel, so that multiple diseases can be tested in one sitting.

But the team has also demonstrated that can transform the testing solution into a powder, making it light and far easier to ship than solutions, which degrade over time. Powder can be stored for years at a time, and offers hope that the technology can be developed into efficient, cheap, over-the-counter tests for diseases such as HIV and malaria for developing countries, where access to portable diagnostics is a necessity.

"We've now put all the pieces together," said Professor Chan.


Story Source:

The above story is based on materials provided by University of Toronto Faculty of Applied Science & Engineering. Note: Materials may be edited for content and length.


Journal Reference:

  1. Kyryl Zagorovsky, Warren C. W. Chan. A Plasmonic DNAzyme Strategy for Point-of-Care Genetic Detection of Infectious Pathogens. Angewandte Chemie International Edition, 2013; DOI: 10.1002/anie.201208715

Cite This Page:

University of Toronto Faculty of Applied Science & Engineering. "Pour, shake and stir: How gold particles, DNA and water have the potential to shape the future of medicine." ScienceDaily. ScienceDaily, 28 February 2013. <www.sciencedaily.com/releases/2013/02/130228171529.htm>.
University of Toronto Faculty of Applied Science & Engineering. (2013, February 28). Pour, shake and stir: How gold particles, DNA and water have the potential to shape the future of medicine. ScienceDaily. Retrieved December 22, 2014 from www.sciencedaily.com/releases/2013/02/130228171529.htm
University of Toronto Faculty of Applied Science & Engineering. "Pour, shake and stir: How gold particles, DNA and water have the potential to shape the future of medicine." ScienceDaily. www.sciencedaily.com/releases/2013/02/130228171529.htm (accessed December 22, 2014).

Share This


More From ScienceDaily



More Matter & Energy News

Monday, December 22, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Will New A350 Help Airbus Fly?

Will New A350 Help Airbus Fly?

Reuters - Business Video Online (Dec. 22, 2014) Qatar Airways takes first delivery of Airbus' new A350 passenger jet. As Joel Flynn reports it's the planemaker's response to the Boeing 787 Dreamliner and the culmination of eight years of development. Video provided by Reuters
Powered by NewsLook.com
Man Parachutes Off Lawn Chair Airlifted By Helium Balloons

Man Parachutes Off Lawn Chair Airlifted By Helium Balloons

Buzz60 (Dec. 22, 2014) A BASE jumper rides a lawn chair, a shotgun, and a giant bunch of helium balloons into the sky in what seems like a country version of the movie 'Up." Jen Markham has the story. Video provided by Buzz60
Powered by NewsLook.com
Touch-Free Smart Phone Empowers Mobility-Impaired

Touch-Free Smart Phone Empowers Mobility-Impaired

Reuters - Innovations Video Online (Dec. 21, 2014) A touch-free phone developed in Israel enables the mobility-impaired to operate smart phones with just a movement of the head. Suzannah Butcher reports. Video provided by Reuters
Powered by NewsLook.com
Existing Chemical Compounds Could Revive Failing Antibiotics, Says Danish Scientist

Existing Chemical Compounds Could Revive Failing Antibiotics, Says Danish Scientist

Reuters - Innovations Video Online (Dec. 21, 2014) A team of scientists led by Danish chemist Jorn Christensen says they have isolated two chemical compounds within an existing antipsychotic medication that could be used to help a range of failing antibiotics work against killer bacterial infections, such as Tuberculosis. Jim Drury went to meet him. Video provided by Reuters
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:

Breaking News:

Strange & Offbeat Stories


Space & Time

Matter & Energy

Computers & Math

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile: iPhone Android Web
Follow: Facebook Twitter Google+
Subscribe: RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins