Featured Research

from universities, journals, and other organizations

Fastest measurements ever made of ion channel proteins

Date:
May 20, 2013
Source:
Columbia University
Summary:
Engineers have used miniaturized electronics to measure the activity of individual ion-channel proteins with temporal resolution as fine as one microsecond, producing the fastest recordings of single ion channels ever performed. They designed a custom integrated circuit to perform these measurements, in which an artificial cell membrane and ion channel are attached directly to the surface of the amplifier chip.

Photograph of integrated amplifiers. The well supporting the membrane and channel is noted in the figure.
Credit: Image courtesy of Columbia University

The miniaturization of electronics continues to create unprecedented capabilities in computer and communications applications, enabling handheld wireless devices with tremendous computing performance operating on battery power. This same miniaturization of electronic systems is also creating new opportunities in biotechnology and biophysics.

A team of researchers at Columbia Engineering has used miniaturized electronics to measure the activity of individual ion-channel proteins with temporal resolution as fine as one microsecond, producing the fastest recordings of single ion channels ever performed. Ion channels are biomolecules that allow charged atoms to flow in and out of cells, and they are an important work-horse in cell signaling, sensing, and energetics. They are also being explored for nanopore sequencing applications. As the "transistors" of living systems, they are the target of many drugs, and the ability to perform such fast measurements of these proteins will lead to new understanding of their functions.

The researchers have designed a custom integrated circuit to perform these measurements, in which an artificial cell membrane and ion channel are attached directly to the surface of the amplifier chip. The results are described in a paper published online May 1, 2013, in Nano Letters.

"Scientists have been measuring single ion channels using large rack-mount electronic systems for the last 30 years," says Jacob Rosenstein, the lead author on the paper. Rosenstein was a PhD student in electrical engineering at the School at the time this work was done, and is now an assistant professor at Brown University. "By designing a custom microelectronic amplifier and tightly integrating the ion channel directly onto the amplifier chip surface, we are able to reduce stray capacitances that get in the way of making fast measurements."

"This work builds on other efforts in my laboratory to study the properties of individual molecules using custom electronics designed for this purpose," says Ken Shepard, professor of electrical engineering at the School and Rosenstein's adviser. The Shepard group continues to find ways to speed up these single-molecule measurements. "In some cases," he adds, "we may be able to speed things up to be a million times faster than current techniques."

This work was funded by the National Institutes of Health and the National Science Foundation.


Story Source:

The above story is based on materials provided by Columbia University. Note: Materials may be edited for content and length.


Journal Reference:

  1. Jacob K. Rosenstein, Siddharth Ramakrishnan, Jared Roseman, Kenneth L. Shepard. Single Ion Channel Recordings with CMOS-Anchored Lipid Membranes. Nano Letters, 2013; 130507161814004 DOI: 10.1021/nl400822r

Cite This Page:

Columbia University. "Fastest measurements ever made of ion channel proteins." ScienceDaily. ScienceDaily, 20 May 2013. <www.sciencedaily.com/releases/2013/05/130520154259.htm>.
Columbia University. (2013, May 20). Fastest measurements ever made of ion channel proteins. ScienceDaily. Retrieved August 1, 2014 from www.sciencedaily.com/releases/2013/05/130520154259.htm
Columbia University. "Fastest measurements ever made of ion channel proteins." ScienceDaily. www.sciencedaily.com/releases/2013/05/130520154259.htm (accessed August 1, 2014).

Share This




More Matter & Energy News

Friday, August 1, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Britain Testing Driverless Cars on Roadways

Britain Testing Driverless Cars on Roadways

AP (July 30, 2014) British officials said on Wednesday that driverless cars will be tested on roads in as many as three cities in a trial program set to begin in January. Officials said the tests will last up to three years. (July 30) Video provided by AP
Powered by NewsLook.com
7 Ways to Use Toothpaste: Howdini Hacks

7 Ways to Use Toothpaste: Howdini Hacks

Howdini (July 30, 2014) Fresh breath and clean teeth are great, but have you ever thought, "my toothpaste could be doing more". Well, it can! Lots of things! Howdini has 7 new uses for this household staple. Video provided by Howdini
Powered by NewsLook.com
Amid Drought, UCLA Sees Only Water

Amid Drought, UCLA Sees Only Water

AP (July 30, 2014) A ruptured 93-year-old water main left the UCLA campus awash in 8 million gallons of water in the middle of California's worst drought in decades. (July 30) Video provided by AP
Powered by NewsLook.com
Smartphone Powered Paper Plane Debuts at Airshow

Smartphone Powered Paper Plane Debuts at Airshow

AP (July 30, 2014) Smartphone powered paper airplane that was popular on crowdfunding website KickStarter makes its debut at Wisconsin airshow (July 30) Video provided by AP
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:

Breaking News:
from the past week

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile: iPhone Android Web
Follow: Facebook Twitter Google+
Subscribe: RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins