Featured Research

from universities, journals, and other organizations

New screening technique paves the way for protein drugs from bacteria

Date:
June 5, 2013
Source:
University of Sheffield
Summary:
A cheaper, more efficient technique for developing complex protein drugs from bacteria has been developed.

A cheaper, more efficient technique for developing complex protein drugs from bacteria has been developed at the University of Sheffield.

Related Articles


Using the bacterium E. coli, researchers from the University's Faculty of Engineering showed it was possible to vastly increase the efficiency of the cells producing specifically modified proteins, as well as improve its performance and stability. The modification is present in over two-thirds of human therapeutic drugs on the market and involves the addition of specific sugar groups to the protein backbone, a process termed glycosylation.

Drugs based on proteins are increasingly important in modern medicine to tackle health problems including diabetes, cancer and arthritis.

Although simple proteins are traditionally made in microbial cells, these types of complex drugs are made using animal cells because they can make human-type glycosylations that will control its efficacy and stability in the body, and avoid immunogenic reactions in patients.

Using bacteria to make proteins for use as medicines could be a more cost effective alternative, since using animal cells is expensive. However, the efficiency of glycoprotein production in bacterial cells is still very poor, with yields often several thousand times lower than in animal cells.

Now, researchers in the Department of Chemical and Biological Engineering at the University of Sheffield, with collaboration from the University of Colorado, are using a technique called inverse metabolic engineering, that allows them to screen cells to identify strains that are likely to be the most efficient glycoprotein producers. Using this method, the team were able to produce seven times as much of the protein in laboratory tests.

The team then used mass spectrometry to characterise and accurately quantify the proteins being produced by the bacteria. This allowed them to pinpoint modifications that will enable them, ultimately, to improve the performance of the drug.

Professor Phil Wright, who led the research, said: "We believe that this technique will pave the way for pharmacologists to get the same protein yield from bacteria cells as they could from animal cells and also enable them to produce drugs from bacteria that have vastly improved focus and accuracy."

The team also tested the technique on antibody fragments with positive results, showing that their approach could work in different proteins.


Story Source:

The above story is based on materials provided by University of Sheffield. Note: Materials may be edited for content and length.


Journal Reference:

  1. Jagroop Pandhal, Lauren B. A. Woodruff, Stephen Jaffe, Pratik Desai, Saw Y. Ow, Josselin Noirel, Ryan T. Gill, Phillip C. Wright. Inverse metabolic engineering to improveEscherichia colias anN-glycosylation host. Biotechnology and Bioengineering, 2013; DOI: 10.1002/bit.24920

Cite This Page:

University of Sheffield. "New screening technique paves the way for protein drugs from bacteria." ScienceDaily. ScienceDaily, 5 June 2013. <www.sciencedaily.com/releases/2013/06/130605104428.htm>.
University of Sheffield. (2013, June 5). New screening technique paves the way for protein drugs from bacteria. ScienceDaily. Retrieved December 18, 2014 from www.sciencedaily.com/releases/2013/06/130605104428.htm
University of Sheffield. "New screening technique paves the way for protein drugs from bacteria." ScienceDaily. www.sciencedaily.com/releases/2013/06/130605104428.htm (accessed December 18, 2014).

Share This


More From ScienceDaily



More Plants & Animals News

Thursday, December 18, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

When You Lose Weight, This Is Where The Fat Goes

When You Lose Weight, This Is Where The Fat Goes

Newsy (Dec. 17, 2014) Can fat disappear into thin air? New research finds that during weight loss, over 80 percent of a person's fat molecules escape through the lungs. Video provided by Newsy
Powered by NewsLook.com
The Hottest Food Trends for 2015

The Hottest Food Trends for 2015

Buzz60 (Dec. 17, 2014) Urbanspoon predicts whicg food trends will dominate the culinary scene in 2015. Mara Montalbano (@maramontalbano) has the story. Video provided by Buzz60
Powered by NewsLook.com
Rover Finds More Clues About Possible Life On Mars

Rover Finds More Clues About Possible Life On Mars

Newsy (Dec. 17, 2014) NASA's Curiosity rover detected methane on Mars and organic compounds on the surface, but it doesn't quite prove there was life ... yet. Video provided by Newsy
Powered by NewsLook.com
Ivory Trade Boom Swamps Law Efforts

Ivory Trade Boom Swamps Law Efforts

Reuters - Business Video Online (Dec. 17, 2014) Demand for ivory has claimed the lives of tens of thousands of African elephants and now a conservation report says the illegal trade is overwhelming efforts to enforce the law. Amy Pollock reports. Video provided by Reuters
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:

Breaking News:

Strange & Offbeat Stories


Plants & Animals

Earth & Climate

Fossils & Ruins

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile: iPhone Android Web
Follow: Facebook Twitter Google+
Subscribe: RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins