Featured Research

from universities, journals, and other organizations

Scientists unexpectedly discover stress-resistant stem cells in fat tissue removed during liposuction

Date:
June 5, 2013
Source:
University of California, Los Angeles (UCLA), Health Sciences
Summary:
Researchers have isolated a new population of primitive, stress-resistant human pluripotent stem cells easily derived from fat tissue that are able to differentiate into virtually every cell type in the human body without genetic modification.

These pluripotent cells, isolated from fat tissue removed during liposuction, expressed many embryonic stem cell markers and were able to differentiate into muscle, bone, fat, cardiac, neuronal and liver cells.
Credit: UCLA

Researchers from the UCLA Department of Obstetrics and Gynecology have isolated a new population of primitive, stress-resistant human pluripotent stem cells easily derived from fat tissue that are able to differentiate into virtually every cell type in the human body without genetic modification.

Related Articles


The cells, called Multi-lineage Stress-Enduring (Muse-AT) stem cells from fat, or adipose, tissue, were discovered by "scientific accident" when a piece of equipment failed in the lab, killing all the stem cells in the experiment except for the Muse-AT cells. The research team further discovered that not only are Muse-AT cells able to survive severe stress, they may even be activated by it, said study senior author Gregorio Chazenbalk, an associate researcher with UCLA Obstetrics and Gynecology.

These pluripotent cells, isolated from fat tissue removed during liposuction, expressed many embryonic stem cell markers and were able to differentiate into muscle, bone, fat, cardiac, neuronal and liver cells. An examination of their genetic characteristics confirmed their specialized functions, as well as their capacity to regenerate tissue when transplanted back into the body following their "awakening."

"This population of cells lies dormant in the fat tissue until it is subjected to very harsh conditions. These cells can survive in conditions in which usually only cancer cells can live," Chazenbalk said. "Upon further investigation and clinical trials, these cells could prove a revolutionary treatment option for numerous diseases, including heart disease, stroke and for tissue damage and neural regeneration."

The results of the two-year study are published June 5, 2013 in the peer-reviewed journal PLOS ONE.

Purifying and isolating Muse-AT cells does not require the use of a cell sorter or other specialized, high-tech devices. They are able to grow either in suspension, forming cell spheres, or as adherent cells, forming cell aggregates very similar to human embryonic stem cell-derived embryoid bodies.

"We have been able to isolate these cells using a simple and efficient method that takes about six hours from the time the fat tissue is harvested," said Chazenbalk,a scientist with the Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research. "This research offers a new and exciting source of fat stem cells with pluripotent characteristics, as well as a new method for quickly isolating them. These cells also appear to be more primitive than the average fat stem cells, making them potentially superior sources for regenerative medicine."

Currently, embryonic stem cells and induced pluripotent stem cells -- skin cells turned into embryonic-like cells -- are the two main sources of pluripotent cells. However, both types can exhibit an uncontrolled capacity for differentiation and proliferation, leading to the formation of unwanted teratoma, or tumors. Little progress has been made in resolving that defect, Chazenbalk said.

Muse cells originally were discovered by a research group at Tokohu University in Japan and were derived from bone marrow and skin, rather than fat. That research group showed that Muse cells did not produce teratomas in animal models. Further research on the Muse-AT cells isolated at UCLA will need to be done to determine whether that cell population avoids production of teratomas.

In addition to providing a potential source of cells for regenerative medicine, Chazenbalk said the Muse-AT cells may provide a better understanding of cancer cells, the only other cells known to display such stress resistance.

Going forward, Chazenbalk and his team will use Muse-AT cells in animal models to regenerate damaged or dysfunctional tissue to determine how efficiently they grow and perform in the body and to gauge their potential for future clinical use.

"Because lipoaspiration is a safe and non-invasive procedure and Muse-AT cell isolation requires a simple yet highly efficient purification technique, Muse-AT cells could provide an ideal source of pluripotent-like stem cells," the study states. "Muse-AT cells have the potential to make a critical impact on the field of regenerative medicine."

The study was funded by the UCLA Department of Obstetrics and Gynecology, the Eunice Kennedy Shriver National Institute of Child Health & Human Development , the National Institutes of Health through the cooperative agreement U54 HD071836 and by the Department of Stem Cell Biology at Tohoku University in Japan.


Story Source:

The above story is based on materials provided by University of California, Los Angeles (UCLA), Health Sciences. Note: Materials may be edited for content and length.


Journal Reference:

  1. Saleh Heneidi, Ariel A. Simerman, Erica Keller, Prapti Singh, Xinmin Li, Daniel A. Dumesic, Gregorio Chazenbalk. Awakened by Cellular Stress: Isolation and Characterization of a Novel Population of Pluripotent Stem Cells Derived from Human Adipose Tissue. PLoS ONE, 2013; 8 (6): e64752 DOI: 10.1371/journal.pone.0064752

Cite This Page:

University of California, Los Angeles (UCLA), Health Sciences. "Scientists unexpectedly discover stress-resistant stem cells in fat tissue removed during liposuction." ScienceDaily. ScienceDaily, 5 June 2013. <www.sciencedaily.com/releases/2013/06/130605185929.htm>.
University of California, Los Angeles (UCLA), Health Sciences. (2013, June 5). Scientists unexpectedly discover stress-resistant stem cells in fat tissue removed during liposuction. ScienceDaily. Retrieved November 27, 2014 from www.sciencedaily.com/releases/2013/06/130605185929.htm
University of California, Los Angeles (UCLA), Health Sciences. "Scientists unexpectedly discover stress-resistant stem cells in fat tissue removed during liposuction." ScienceDaily. www.sciencedaily.com/releases/2013/06/130605185929.htm (accessed November 27, 2014).

Share This


More From ScienceDaily



More Health & Medicine News

Thursday, November 27, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Ebola Leaves Orphans Alone in Sierra Leone

Ebola Leaves Orphans Alone in Sierra Leone

AFP (Nov. 27, 2014) — The Ebola epidemic sweeping Sierra Leone is having a profound effect on the country's children, many of whom have been left without any family members to support them. Duration: 01:02 Video provided by AFP
Powered by NewsLook.com
Experimental Ebola Vaccine Shows Promise In Human Trial

Experimental Ebola Vaccine Shows Promise In Human Trial

Newsy (Nov. 27, 2014) — A recent test of a prototype Ebola vaccine generated an immune response to the disease in subjects. Video provided by Newsy
Powered by NewsLook.com
Pet Dogs to Be Used in Anti-Ageing Trial

Pet Dogs to Be Used in Anti-Ageing Trial

Reuters - Innovations Video Online (Nov. 26, 2014) — Researchers in the United States are preparing to discover whether a drug commonly used in human organ transplants can extend the lifespan and health quality of pet dogs. Video provided by Reuters
Powered by NewsLook.com
Today's Prostheses Are More Capable Than Ever

Today's Prostheses Are More Capable Than Ever

Newsy (Nov. 26, 2014) — Advances in prosthetics are making replacement body parts stronger and more lifelike than they’ve ever been. Video provided by Newsy
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
 
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:  

Breaking News:

Strange & Offbeat Stories

 

Health & Medicine

Mind & Brain

Living & Well

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:  

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile iPhone Android Web
Follow Facebook Twitter Google+
Subscribe RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins